Antioxidant Activity of Cosmos Caudatus Extracts By Using Different Types of Extraction Methods

The discoveries on new resources of natural antioxidant from plants have attracted increasing interest due to high potential of antioxidant capacity. In this paper, antioxidant activity of Cosmos caudatus extracts was investigated by using different types of extraction methods which were maceration...

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Main Author:	Nur Afeefa, Mohd Khairi
Format:	Undergraduates Project Papers
Language:	English
Published:	2013
Subjects:	TX Home economics
Online Access:	http://umpir.ump.edu.my/id/eprint/7027/ http://umpir.ump.edu.my/id/eprint/7027/ http://umpir.ump.edu.my/id/eprint/7027/1/CD7336.pdf

id	ump-7027
recordtype	eprints
spelling	ump-70272015-03-03T09:33:55Z http://umpir.ump.edu.my/id/eprint/7027/ Antioxidant Activity of Cosmos Caudatus Extracts By Using Different Types of Extraction Methods Nur Afeefa, Mohd Khairi TX Home economics The discoveries on new resources of natural antioxidant from plants have attracted increasing interest due to high potential of antioxidant capacity. In this paper, antioxidant activity of Cosmos caudatus extracts was investigated by using different types of extraction methods which were maceration extraction, Soxhlet extraction and ultrasonic-assisted extraction using 70% acetone (v/v) as solvent. The antioxidant activity and ascorbic acid content from each method was determined by 2,2-Diphenyl-1-Picrylhydrazyl (DPPH) assay and High Performance Liquid Chromatography (HPLC) respectively. The results show that the three extraction methods exhibit significant value of ascorbic acid content. Ultrasonic-assisted extraction was the efficient method as it exhibits the highest amount of ascorbic acid content with 26.590 mg ascorbic acid equivalent per 1 g sample (mg AAE/g) with the corresponding value of 42.208% of its antioxidant activity after 60 minutes extraction. This is followed by Soxhlet extraction (13.527 mg AAE/g) and maceration extraction (5.190 mg AAE/g) within 8 hour and 24 hour extraction time respectively. Analysis by HPLC also shows that C. caudatus extract using ultrasonicassisted extraction has the highest vitamin C content (2.440 mg AAE/g) followed by Soxhlet extraction (0.968 mg AAE/g) and maceration extraction (0.739 mg AAE/g). For further study, optimization of the ultrasonic extraction method is proposed to achieve the optimum condition in extracting bioactive compounds from C. caudatus. 2013-02 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/7027/1/CD7336.pdf Nur Afeefa, Mohd Khairi (2013) Antioxidant Activity of Cosmos Caudatus Extracts By Using Different Types of Extraction Methods. Faculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:75701&theme=UMP2
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TX Home economics
spellingShingle	TX Home economics Nur Afeefa, Mohd Khairi Antioxidant Activity of Cosmos Caudatus Extracts By Using Different Types of Extraction Methods
description	The discoveries on new resources of natural antioxidant from plants have attracted increasing interest due to high potential of antioxidant capacity. In this paper, antioxidant activity of Cosmos caudatus extracts was investigated by using different types of extraction methods which were maceration extraction, Soxhlet extraction and ultrasonic-assisted extraction using 70% acetone (v/v) as solvent. The antioxidant activity and ascorbic acid content from each method was determined by 2,2-Diphenyl-1-Picrylhydrazyl (DPPH) assay and High Performance Liquid Chromatography (HPLC) respectively. The results show that the three extraction methods exhibit significant value of ascorbic acid content. Ultrasonic-assisted extraction was the efficient method as it exhibits the highest amount of ascorbic acid content with 26.590 mg ascorbic acid equivalent per 1 g sample (mg AAE/g) with the corresponding value of 42.208% of its antioxidant activity after 60 minutes extraction. This is followed by Soxhlet extraction (13.527 mg AAE/g) and maceration extraction (5.190 mg AAE/g) within 8 hour and 24 hour extraction time respectively. Analysis by HPLC also shows that C. caudatus extract using ultrasonicassisted extraction has the highest vitamin C content (2.440 mg AAE/g) followed by Soxhlet extraction (0.968 mg AAE/g) and maceration extraction (0.739 mg AAE/g). For further study, optimization of the ultrasonic extraction method is proposed to achieve the optimum condition in extracting bioactive compounds from C. caudatus.
format	Undergraduates Project Papers
author	Nur Afeefa, Mohd Khairi
author_facet	Nur Afeefa, Mohd Khairi
author_sort	Nur Afeefa, Mohd Khairi
title	Antioxidant Activity of Cosmos Caudatus Extracts By Using Different Types of Extraction Methods
title_short	Antioxidant Activity of Cosmos Caudatus Extracts By Using Different Types of Extraction Methods
title_full	Antioxidant Activity of Cosmos Caudatus Extracts By Using Different Types of Extraction Methods
title_fullStr	Antioxidant Activity of Cosmos Caudatus Extracts By Using Different Types of Extraction Methods
title_full_unstemmed	Antioxidant Activity of Cosmos Caudatus Extracts By Using Different Types of Extraction Methods
title_sort	antioxidant activity of cosmos caudatus extracts by using different types of extraction methods
publishDate	2013
url	http://umpir.ump.edu.my/id/eprint/7027/ http://umpir.ump.edu.my/id/eprint/7027/ http://umpir.ump.edu.my/id/eprint/7027/1/CD7336.pdf
first_indexed	2023-09-18T22:03:17Z
last_indexed	2023-09-18T22:03:17Z
_version_	1777414541159694336

Antioxidant Activity of Cosmos Caudatus Extracts By Using Different Types of Extraction Methods

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