Adsorption Study of Fructosyltransferase Immobilization By Using Various Adsorbent

Immobilized enzyme are widely used in the industry because of its stability and economic. Because of the high demand from the industry, the research is done in order to enhance the quality of immobilized enzyme. This study discusses about the adsorption in the enzyme immobilization process. The purp...

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Main Author:	Nor Hazwani Syuhada, Azmi
Format:	Undergraduates Project Papers
Language:	English
Published:	2013
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/7036/ http://umpir.ump.edu.my/id/eprint/7036/ http://umpir.ump.edu.my/id/eprint/7036/1/CD7332.pdf

id	ump-7036
recordtype	eprints
spelling	ump-70362015-03-03T09:33:58Z http://umpir.ump.edu.my/id/eprint/7036/ Adsorption Study of Fructosyltransferase Immobilization By Using Various Adsorbent Nor Hazwani Syuhada, Azmi TP Chemical technology Immobilized enzyme are widely used in the industry because of its stability and economic. Because of the high demand from the industry, the research is done in order to enhance the quality of immobilized enzyme. This study discusses about the adsorption in the enzyme immobilization process. The purposes of this study are to find out the species of sawdust (Keruing, Meranti and Kempas) which have the excellent adsorption capability, to investigate the effect of various initial enzyme concentrations and the time required for the adsorption of adsorbate onto adsorbent reach equilibrium. For the immobilization process, it was occurred in water bath shaker for 26 h at room temperature and at pH 5.5. The enzyme activity was analyzed by using DNS method. To determine the equilibrium time, the solutions was being agitated in water bath shaker at 100 rpm and the samples were taken out at a given time intervals for analyses (0-40 hours). The concentration of FTase was analyzed by using UV-Vis Spectrometer. The effect of various initial enzyme concentrations (0, 2, 4, 6, 8, 10, 12, and 14 g/L) in the adsorption process was analyzed by using UV-Vis Spectrometer and employing Langmuir and Freundlich models in order to find out the isotherm which fit with the data obtained.SEM and FTIR was done to identify the structures of the untreated, treated sawdust and immobilize enzyme. All the three adsorbents had achieved the equilibrium at time 25 to 30 hours and it is showed that Keruing adsorbent comes out with the highest loading capacity (0.945g of FTase/L) due to the size of pores itself. Enzyme concentration residue increases after the addition of 6 g/L of FTase due to the amount of enzyme were exceeds the amount of adsorbent. Freundlich isotherms fitted the data while Keruing showed the highest capability of adsorption followed by Meranti as adsorbent. 2013-02 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/7036/1/CD7332.pdf Nor Hazwani Syuhada, Azmi (2013) Adsorption Study of Fructosyltransferase Immobilization By Using Various Adsorbent. Faculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:75800&theme=UMP2
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Nor Hazwani Syuhada, Azmi Adsorption Study of Fructosyltransferase Immobilization By Using Various Adsorbent
description	Immobilized enzyme are widely used in the industry because of its stability and economic. Because of the high demand from the industry, the research is done in order to enhance the quality of immobilized enzyme. This study discusses about the adsorption in the enzyme immobilization process. The purposes of this study are to find out the species of sawdust (Keruing, Meranti and Kempas) which have the excellent adsorption capability, to investigate the effect of various initial enzyme concentrations and the time required for the adsorption of adsorbate onto adsorbent reach equilibrium. For the immobilization process, it was occurred in water bath shaker for 26 h at room temperature and at pH 5.5. The enzyme activity was analyzed by using DNS method. To determine the equilibrium time, the solutions was being agitated in water bath shaker at 100 rpm and the samples were taken out at a given time intervals for analyses (0-40 hours). The concentration of FTase was analyzed by using UV-Vis Spectrometer. The effect of various initial enzyme concentrations (0, 2, 4, 6, 8, 10, 12, and 14 g/L) in the adsorption process was analyzed by using UV-Vis Spectrometer and employing Langmuir and Freundlich models in order to find out the isotherm which fit with the data obtained.SEM and FTIR was done to identify the structures of the untreated, treated sawdust and immobilize enzyme. All the three adsorbents had achieved the equilibrium at time 25 to 30 hours and it is showed that Keruing adsorbent comes out with the highest loading capacity (0.945g of FTase/L) due to the size of pores itself. Enzyme concentration residue increases after the addition of 6 g/L of FTase due to the amount of enzyme were exceeds the amount of adsorbent. Freundlich isotherms fitted the data while Keruing showed the highest capability of adsorption followed by Meranti as adsorbent.
format	Undergraduates Project Papers
author	Nor Hazwani Syuhada, Azmi
author_facet	Nor Hazwani Syuhada, Azmi
author_sort	Nor Hazwani Syuhada, Azmi
title	Adsorption Study of Fructosyltransferase Immobilization By Using Various Adsorbent
title_short	Adsorption Study of Fructosyltransferase Immobilization By Using Various Adsorbent
title_full	Adsorption Study of Fructosyltransferase Immobilization By Using Various Adsorbent
title_fullStr	Adsorption Study of Fructosyltransferase Immobilization By Using Various Adsorbent
title_full_unstemmed	Adsorption Study of Fructosyltransferase Immobilization By Using Various Adsorbent
title_sort	adsorption study of fructosyltransferase immobilization by using various adsorbent
publishDate	2013
url	http://umpir.ump.edu.my/id/eprint/7036/ http://umpir.ump.edu.my/id/eprint/7036/ http://umpir.ump.edu.my/id/eprint/7036/1/CD7332.pdf
first_indexed	2023-09-18T22:03:18Z
last_indexed	2023-09-18T22:03:18Z
_version_	1777414542491385856

Adsorption Study of Fructosyltransferase Immobilization By Using Various Adsorbent

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