Fermentative Production of Butanol From Oil Palm Trunk (OPT) Sap Using Clostridium Acetobutylicum and Clostridium Beijerinckii

Fermentative Production of Butanol From Oil Palm Trunk (OPT) Sap Using Clostridium Acetobutylicum and Clostridium Beijerinckii

Malaysia is the one of world’s largest producer of palm oil. Waste from palm oil production factories has been increasing rapidly and one of the serious problems in the palm oil fruit processing is managing the waste generated by the processes. Recent studies showed that butanol can be used as excel...

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Main Author: Farhanah, Amanulla Khan
Format: Undergraduates Project Papers
Language:English
Published: 2013
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/7043/
http://umpir.ump.edu.my/id/eprint/7043/
http://umpir.ump.edu.my/id/eprint/7043/1/CD7326.pdf
id ump-7043
recordtype eprints
spelling ump-70432015-03-03T09:34:01Z http://umpir.ump.edu.my/id/eprint/7043/ Fermentative Production of Butanol From Oil Palm Trunk (OPT) Sap Using Clostridium Acetobutylicum and Clostridium Beijerinckii Farhanah, Amanulla Khan TP Chemical technology Malaysia is the one of world’s largest producer of palm oil. Waste from palm oil production factories has been increasing rapidly and one of the serious problems in the palm oil fruit processing is managing the waste generated by the processes. Recent studies showed that butanol can be used as excellent renewable liquid transportation fuel. In this study, oil palm trunk sap is used as the substrate. The objective of this study is to compare the production of biobutanol from biomass waste (oil palm trunk sap) via fermentation process using different microorganisms. The fermentation type done was submerged fermentation. Fermentation was conducted for five days at 37 ⁰C. Samples were taken for every 6 hours for 3 days, then for every 12 hours. Samples taken were analyzed using gas chromatography-flame ionization detector (GC-FID) and high performance liquid chromatography (HPLC) was used to analyze sugar composition in OPT sap. UV Spectrophotometer is used to analyze biomass. Oil palm trunk (OPT) sap contains sucrose as its dominant sugar followed by glucose, fructose and galactose. Based on the analysis, acetone, butanol and ethanol were produced. Comparisons between the two strains Clostridium acetobutylicum and Clostridium beijerinckii were made, and C. beijerinckii showed higher production of acetone, butanol and ethanol. The highest yield for butanol production by C. acetobutylicum and C. beijerinckii were 13.910 g/L and 15.301 g/L and highest production of ethanol were 11.17 g/L and 12.09 g/L. 4.13 g/L and 4.49 g/L acetone was produced by C. acetobutylicum and C. beijerinckii. Based on this study, it is concluded that OPT sap is preferable substrate to produce butanol by using C. beijerinckii. 2013-02 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/7043/1/CD7326.pdf Farhanah, Amanulla Khan (2013) Fermentative Production of Butanol From Oil Palm Trunk (OPT) Sap Using Clostridium Acetobutylicum and Clostridium Beijerinckii. Faculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:75692&theme=UMP2
repository_type Digital Repository
institution_category Local University
institution Universiti Malaysia Pahang
building UMP Institutional Repository
collection Online Access
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Farhanah, Amanulla Khan
Fermentative Production of Butanol From Oil Palm Trunk (OPT) Sap Using Clostridium Acetobutylicum and Clostridium Beijerinckii
description Malaysia is the one of world’s largest producer of palm oil. Waste from palm oil production factories has been increasing rapidly and one of the serious problems in the palm oil fruit processing is managing the waste generated by the processes. Recent studies showed that butanol can be used as excellent renewable liquid transportation fuel. In this study, oil palm trunk sap is used as the substrate. The objective of this study is to compare the production of biobutanol from biomass waste (oil palm trunk sap) via fermentation process using different microorganisms. The fermentation type done was submerged fermentation. Fermentation was conducted for five days at 37 ⁰C. Samples were taken for every 6 hours for 3 days, then for every 12 hours. Samples taken were analyzed using gas chromatography-flame ionization detector (GC-FID) and high performance liquid chromatography (HPLC) was used to analyze sugar composition in OPT sap. UV Spectrophotometer is used to analyze biomass. Oil palm trunk (OPT) sap contains sucrose as its dominant sugar followed by glucose, fructose and galactose. Based on the analysis, acetone, butanol and ethanol were produced. Comparisons between the two strains Clostridium acetobutylicum and Clostridium beijerinckii were made, and C. beijerinckii showed higher production of acetone, butanol and ethanol. The highest yield for butanol production by C. acetobutylicum and C. beijerinckii were 13.910 g/L and 15.301 g/L and highest production of ethanol were 11.17 g/L and 12.09 g/L. 4.13 g/L and 4.49 g/L acetone was produced by C. acetobutylicum and C. beijerinckii. Based on this study, it is concluded that OPT sap is preferable substrate to produce butanol by using C. beijerinckii.
format Undergraduates Project Papers
author Farhanah, Amanulla Khan
author_facet Farhanah, Amanulla Khan
author_sort Farhanah, Amanulla Khan
title Fermentative Production of Butanol From Oil Palm Trunk (OPT) Sap Using Clostridium Acetobutylicum and Clostridium Beijerinckii
title_short Fermentative Production of Butanol From Oil Palm Trunk (OPT) Sap Using Clostridium Acetobutylicum and Clostridium Beijerinckii
title_full Fermentative Production of Butanol From Oil Palm Trunk (OPT) Sap Using Clostridium Acetobutylicum and Clostridium Beijerinckii
title_fullStr Fermentative Production of Butanol From Oil Palm Trunk (OPT) Sap Using Clostridium Acetobutylicum and Clostridium Beijerinckii
title_full_unstemmed Fermentative Production of Butanol From Oil Palm Trunk (OPT) Sap Using Clostridium Acetobutylicum and Clostridium Beijerinckii
title_sort fermentative production of butanol from oil palm trunk (opt) sap using clostridium acetobutylicum and clostridium beijerinckii
publishDate 2013
url http://umpir.ump.edu.my/id/eprint/7043/
http://umpir.ump.edu.my/id/eprint/7043/
http://umpir.ump.edu.my/id/eprint/7043/1/CD7326.pdf
first_indexed 2023-09-18T22:03:19Z
last_indexed 2023-09-18T22:03:19Z
_version_ 1777414543531573248

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