Quantification of bioactive compounds from Averrhoa Bilimbi

Quantification of bioactive compounds from Averrhoa Bilimbi

Averrhoa bilimbi has been widely used in traditional medicine, thus, this fruits have received much attention because of its nutritional and antioxidant properties. The purposes of this study are to determine the bioactive compounds in averrhoa bilimbi and to study their optimum parameters during ex...

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Main Author: Siti Aishah, Muhmed
Format: Undergraduates Project Papers
Language:English
Published: 2013
Subjects:
QK Botany
Online Access:http://umpir.ump.edu.my/id/eprint/7144/
http://umpir.ump.edu.my/id/eprint/7144/
http://umpir.ump.edu.my/id/eprint/7144/1/Quantification_of_bioactive_compounds_from_Averrhoa_Bilimbi.pdf
id ump-7144
recordtype eprints
spelling ump-71442015-03-03T09:34:29Z http://umpir.ump.edu.my/id/eprint/7144/ Quantification of bioactive compounds from Averrhoa Bilimbi Siti Aishah, Muhmed QK Botany Averrhoa bilimbi has been widely used in traditional medicine, thus, this fruits have received much attention because of its nutritional and antioxidant properties. The purposes of this study are to determine the bioactive compounds in averrhoa bilimbi and to study their optimum parameters during extraction. Antioxidant activity of the averrhoa bilimbi extract was determined based on 2,2-diphenyl-1-picrylhydrazyl freeradical (DPPH·), total phenolic content was measured using Folin–Ciocalteu reagent, while flavonoids is determined spectrophotometrically. Solvent extraction selected is methanol (50%) where this condition has yielded total flavonoids content is about 568.75 μg/ml QE. The highest antioxidant capacities measured is 80.02 % and total phenolic content shows about 175.3 mg/mL GAE.In this study, 60 minutes extraction time generally showed the highest effect on total flavonoids which is 496.25 μg/ml QE. Averrhoa bilimbi extraction had total phenolic about 164.7 mg/mL GAE and antioxidant activity is around 74.5%. 70° C extraction temperature shown the best extraction for total phenolics at 127.7 mg/ml GAE, while achieved highest total flavonoids at 656.25 μg/ml QE and the highest for DPPH radical scavenging activity is found about 70.05%. From the effect of agitation speed, 300 rpm achieved the highest value for total phenolics, total flavonoids and antioxidant capacity, DPPH which is about 193.3 mg/ml GAE, 717.75 μg/ml QE, and 77.03% respectively. Overall, based on the ideal extraction conditions chosen, optimum level of TPC, TFC and antioxidant capacity were obtained in averrhoa bilimbi fruit extract. The selected extraction conditions could be used for further studies and functional food product development. 2013 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/7144/1/Quantification_of_bioactive_compounds_from_Averrhoa_Bilimbi.pdf Siti Aishah, Muhmed (2013) Quantification of bioactive compounds from Averrhoa Bilimbi. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:75792&theme=UMP2
repository_type Digital Repository
institution_category Local University
institution Universiti Malaysia Pahang
building UMP Institutional Repository
collection Online Access
language English
topic QK Botany
spellingShingle QK Botany
Siti Aishah, Muhmed
Quantification of bioactive compounds from Averrhoa Bilimbi
description Averrhoa bilimbi has been widely used in traditional medicine, thus, this fruits have received much attention because of its nutritional and antioxidant properties. The purposes of this study are to determine the bioactive compounds in averrhoa bilimbi and to study their optimum parameters during extraction. Antioxidant activity of the averrhoa bilimbi extract was determined based on 2,2-diphenyl-1-picrylhydrazyl freeradical (DPPH·), total phenolic content was measured using Folin–Ciocalteu reagent, while flavonoids is determined spectrophotometrically. Solvent extraction selected is methanol (50%) where this condition has yielded total flavonoids content is about 568.75 μg/ml QE. The highest antioxidant capacities measured is 80.02 % and total phenolic content shows about 175.3 mg/mL GAE.In this study, 60 minutes extraction time generally showed the highest effect on total flavonoids which is 496.25 μg/ml QE. Averrhoa bilimbi extraction had total phenolic about 164.7 mg/mL GAE and antioxidant activity is around 74.5%. 70° C extraction temperature shown the best extraction for total phenolics at 127.7 mg/ml GAE, while achieved highest total flavonoids at 656.25 μg/ml QE and the highest for DPPH radical scavenging activity is found about 70.05%. From the effect of agitation speed, 300 rpm achieved the highest value for total phenolics, total flavonoids and antioxidant capacity, DPPH which is about 193.3 mg/ml GAE, 717.75 μg/ml QE, and 77.03% respectively. Overall, based on the ideal extraction conditions chosen, optimum level of TPC, TFC and antioxidant capacity were obtained in averrhoa bilimbi fruit extract. The selected extraction conditions could be used for further studies and functional food product development.
format Undergraduates Project Papers
author Siti Aishah, Muhmed
author_facet Siti Aishah, Muhmed
author_sort Siti Aishah, Muhmed
title Quantification of bioactive compounds from Averrhoa Bilimbi
title_short Quantification of bioactive compounds from Averrhoa Bilimbi
title_full Quantification of bioactive compounds from Averrhoa Bilimbi
title_fullStr Quantification of bioactive compounds from Averrhoa Bilimbi
title_full_unstemmed Quantification of bioactive compounds from Averrhoa Bilimbi
title_sort quantification of bioactive compounds from averrhoa bilimbi
publishDate 2013
url http://umpir.ump.edu.my/id/eprint/7144/
http://umpir.ump.edu.my/id/eprint/7144/
http://umpir.ump.edu.my/id/eprint/7144/1/Quantification_of_bioactive_compounds_from_Averrhoa_Bilimbi.pdf
first_indexed 2023-09-18T22:03:33Z
last_indexed 2023-09-18T22:03:33Z
_version_ 1777414558168645632

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