Study the Reaction Mechanism of The Production of Novel Biopolymer [Poly (3-Hydroxybutyrate) (PHB)] from the Palm Oil Seed

Poly(3-hyroxybutyrate) (PHB), is a biodegradable plastic that recognized as a potential alternative to substitute conventional petrochemical-based plastic. This biodegradable plastic is derived from bacterial fermentation using various carbon sources. Since the cost of carbon substrates has been ide...

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Main Author: Mohd Khairul Nizam, Baharudin
Format: Undergraduates Project Papers
Language:English
Published: 2012
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/7153/
http://umpir.ump.edu.my/id/eprint/7153/
http://umpir.ump.edu.my/id/eprint/7153/1/CD7150.pdf
id ump-7153
recordtype eprints
spelling ump-71532015-03-03T09:34:32Z http://umpir.ump.edu.my/id/eprint/7153/ Study the Reaction Mechanism of The Production of Novel Biopolymer [Poly (3-Hydroxybutyrate) (PHB)] from the Palm Oil Seed Mohd Khairul Nizam, Baharudin TP Chemical technology Poly(3-hyroxybutyrate) (PHB), is a biodegradable plastic that recognized as a potential alternative to substitute conventional petrochemical-based plastic. This biodegradable plastic is derived from bacterial fermentation using various carbon sources. Since the cost of carbon substrates has been identified as the major constrain in PHB production, inexpensive and renewable carbon substrate were currently investigated as alternative to conventional sugar-based substrate. Palm oil, massive-readily edible plant oil, may be an alternative substrate to conventional carbon source for PHB production. PHB is synthesized by Alcaligenes eutrophus in a production medium containing palm oil. Highest cell-dry weight was obtained by A. eutrophus when 20 g/L of palm oil and 0.6 g/L of urea were used. Cell-dry weight increased when the addition of starter inoculum volume increased up to 10 mL. Highest cell-dry weight was obtained at 48 to 60 hour of incubation. In a cell growth kinetic study, the total cell-dry weight obtained in presence of 20 g/L palm oil was greater than 12g/L and 16 g/L. Bacteria fermentation in the presence of 0.6 g/L urea shows greater total cell-dry weight compared to fermentation system without any addition of urea. This study proved that palm oil is a feasible and excellence carbon substrate for PHB biosynthesis by A. eutrophus. In this study, the evaluation of optimum condition for PHB biosynthesis by bacterial fermentation was accomplished. 2012-06 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/7153/1/CD7150.pdf Mohd Khairul Nizam, Baharudin (2012) Study the Reaction Mechanism of The Production of Novel Biopolymer [Poly (3-Hydroxybutyrate) (PHB)] from the Palm Oil Seed. Faculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:75089&theme=UMP2
repository_type Digital Repository
institution_category Local University
institution Universiti Malaysia Pahang
building UMP Institutional Repository
collection Online Access
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Mohd Khairul Nizam, Baharudin
Study the Reaction Mechanism of The Production of Novel Biopolymer [Poly (3-Hydroxybutyrate) (PHB)] from the Palm Oil Seed
description Poly(3-hyroxybutyrate) (PHB), is a biodegradable plastic that recognized as a potential alternative to substitute conventional petrochemical-based plastic. This biodegradable plastic is derived from bacterial fermentation using various carbon sources. Since the cost of carbon substrates has been identified as the major constrain in PHB production, inexpensive and renewable carbon substrate were currently investigated as alternative to conventional sugar-based substrate. Palm oil, massive-readily edible plant oil, may be an alternative substrate to conventional carbon source for PHB production. PHB is synthesized by Alcaligenes eutrophus in a production medium containing palm oil. Highest cell-dry weight was obtained by A. eutrophus when 20 g/L of palm oil and 0.6 g/L of urea were used. Cell-dry weight increased when the addition of starter inoculum volume increased up to 10 mL. Highest cell-dry weight was obtained at 48 to 60 hour of incubation. In a cell growth kinetic study, the total cell-dry weight obtained in presence of 20 g/L palm oil was greater than 12g/L and 16 g/L. Bacteria fermentation in the presence of 0.6 g/L urea shows greater total cell-dry weight compared to fermentation system without any addition of urea. This study proved that palm oil is a feasible and excellence carbon substrate for PHB biosynthesis by A. eutrophus. In this study, the evaluation of optimum condition for PHB biosynthesis by bacterial fermentation was accomplished.
format Undergraduates Project Papers
author Mohd Khairul Nizam, Baharudin
author_facet Mohd Khairul Nizam, Baharudin
author_sort Mohd Khairul Nizam, Baharudin
title Study the Reaction Mechanism of The Production of Novel Biopolymer [Poly (3-Hydroxybutyrate) (PHB)] from the Palm Oil Seed
title_short Study the Reaction Mechanism of The Production of Novel Biopolymer [Poly (3-Hydroxybutyrate) (PHB)] from the Palm Oil Seed
title_full Study the Reaction Mechanism of The Production of Novel Biopolymer [Poly (3-Hydroxybutyrate) (PHB)] from the Palm Oil Seed
title_fullStr Study the Reaction Mechanism of The Production of Novel Biopolymer [Poly (3-Hydroxybutyrate) (PHB)] from the Palm Oil Seed
title_full_unstemmed Study the Reaction Mechanism of The Production of Novel Biopolymer [Poly (3-Hydroxybutyrate) (PHB)] from the Palm Oil Seed
title_sort study the reaction mechanism of the production of novel biopolymer [poly (3-hydroxybutyrate) (phb)] from the palm oil seed
publishDate 2012
url http://umpir.ump.edu.my/id/eprint/7153/
http://umpir.ump.edu.my/id/eprint/7153/
http://umpir.ump.edu.my/id/eprint/7153/1/CD7150.pdf
first_indexed 2023-09-18T22:03:34Z
last_indexed 2023-09-18T22:03:34Z
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