Development of anion-exchange membrane chromatography from regenerated cellulose membrane by attaching different spacer arm length of diamine monomer

Chromatography technique is widely used for protein separation. Conventional packed bed column chromatography has several limitations. Membrane chromatography was a suitable alternative technique for protein separation. Specific monomer can be grafted to uncharged membrane to transform into membrane...

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Main Author:	Lee, Yue Wei
Format:	Undergraduates Project Papers
Language:	English
Published:	2013
Subjects:	QD Chemistry
Online Access:	http://umpir.ump.edu.my/id/eprint/7169/ http://umpir.ump.edu.my/id/eprint/7169/ http://umpir.ump.edu.my/id/eprint/7169/1/Development_of_anion-exchange_membrane_chromatography.pdf

id	ump-7169
recordtype	eprints
spelling	ump-71692015-03-03T09:34:36Z http://umpir.ump.edu.my/id/eprint/7169/ Development of anion-exchange membrane chromatography from regenerated cellulose membrane by attaching different spacer arm length of diamine monomer Lee, Yue Wei QD Chemistry Chromatography technique is widely used for protein separation. Conventional packed bed column chromatography has several limitations. Membrane chromatography was a suitable alternative technique for protein separation. Specific monomer can be grafted to uncharged membrane to transform into membrane chromatography material. Optimization of parameters involve during this chemical modification is crucial for the development of high performance membrane chromatography for protein separation. The purpose of this research is to develop anion-exchange membrane chromatography from regenerated cellulose membrane by attaching different spacer arm lengths of diamine monomer. Regenerated cellulose membrane was activated in a solution containing sodium hydroxide (NaOH) and epichlorohydrin (EPI). Then, the membrane was immersed in diamine solution of 1,2-diaminoethane or 1,4-diaminobutanhe to produce positively charged membrane chromatography. The concentration of NaOH activation from 0.05M to 0.50M and diamine monomer concentration from 0.25M to 2.0M during grafting were studied. The optimum concentration of NaOH was 0.20M which produced anion exchange membrane capacity of 0.310±0.033 mgBSA/cm2 membrane. High concentration of diamine monomer at 2.0M 1,4-diaminobutane showed a binding capacity of 0.385±0.027mgBSA/cm2 membrane. Based on FTIR transmission peak, both N-H and C-N functional groups were detected in modified membrane that indicated the successful of grafting process. 2013 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/7169/1/Development_of_anion-exchange_membrane_chromatography.pdf Lee, Yue Wei (2013) Development of anion-exchange membrane chromatography from regenerated cellulose membrane by attaching different spacer arm length of diamine monomer. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:75750&theme=UMP2
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	QD Chemistry
spellingShingle	QD Chemistry Lee, Yue Wei Development of anion-exchange membrane chromatography from regenerated cellulose membrane by attaching different spacer arm length of diamine monomer
description	Chromatography technique is widely used for protein separation. Conventional packed bed column chromatography has several limitations. Membrane chromatography was a suitable alternative technique for protein separation. Specific monomer can be grafted to uncharged membrane to transform into membrane chromatography material. Optimization of parameters involve during this chemical modification is crucial for the development of high performance membrane chromatography for protein separation. The purpose of this research is to develop anion-exchange membrane chromatography from regenerated cellulose membrane by attaching different spacer arm lengths of diamine monomer. Regenerated cellulose membrane was activated in a solution containing sodium hydroxide (NaOH) and epichlorohydrin (EPI). Then, the membrane was immersed in diamine solution of 1,2-diaminoethane or 1,4-diaminobutanhe to produce positively charged membrane chromatography. The concentration of NaOH activation from 0.05M to 0.50M and diamine monomer concentration from 0.25M to 2.0M during grafting were studied. The optimum concentration of NaOH was 0.20M which produced anion exchange membrane capacity of 0.310±0.033 mgBSA/cm2 membrane. High concentration of diamine monomer at 2.0M 1,4-diaminobutane showed a binding capacity of 0.385±0.027mgBSA/cm2 membrane. Based on FTIR transmission peak, both N-H and C-N functional groups were detected in modified membrane that indicated the successful of grafting process.
format	Undergraduates Project Papers
author	Lee, Yue Wei
author_facet	Lee, Yue Wei
author_sort	Lee, Yue Wei
title	Development of anion-exchange membrane chromatography from regenerated cellulose membrane by attaching different spacer arm length of diamine monomer
title_short	Development of anion-exchange membrane chromatography from regenerated cellulose membrane by attaching different spacer arm length of diamine monomer
title_full	Development of anion-exchange membrane chromatography from regenerated cellulose membrane by attaching different spacer arm length of diamine monomer
title_fullStr	Development of anion-exchange membrane chromatography from regenerated cellulose membrane by attaching different spacer arm length of diamine monomer
title_full_unstemmed	Development of anion-exchange membrane chromatography from regenerated cellulose membrane by attaching different spacer arm length of diamine monomer
title_sort	development of anion-exchange membrane chromatography from regenerated cellulose membrane by attaching different spacer arm length of diamine monomer
publishDate	2013
url	http://umpir.ump.edu.my/id/eprint/7169/ http://umpir.ump.edu.my/id/eprint/7169/ http://umpir.ump.edu.my/id/eprint/7169/1/Development_of_anion-exchange_membrane_chromatography.pdf
first_indexed	2023-09-18T22:03:37Z
last_indexed	2023-09-18T22:03:37Z
_version_	1777414561792524288

Development of anion-exchange membrane chromatography from regenerated cellulose membrane by attaching different spacer arm length of diamine monomer

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