A comparative study on milk protein separation using different technique
Fractionation of full cream milk protein using two protein separation techniques which are crossflow filtration and conventional method was examined. Two membrane of MWCO of 10 and 50 kDa of polyethersulfone material were used to determine the efficiency of the process for crossflow filtration and f...
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| Format: | Undergraduates Project Papers |
| Language: | English |
| Published: |
2009
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| Online Access: | http://umpir.ump.edu.my/id/eprint/736/ http://umpir.ump.edu.my/id/eprint/736/ http://umpir.ump.edu.my/id/eprint/736/1/Wan_Nurfarah_Wan_Ali.pdf |
| Summary: | Fractionation of full cream milk protein using two protein separation techniques which are crossflow filtration and conventional method was examined. Two membrane of MWCO of 10 and 50 kDa of polyethersulfone material were used to determine the efficiency of the process for crossflow filtration and filter paper were used for conventional method. The performance of crossflow filtration was determined under various processing conditions that include the transmembrane pressure across the membrane and the concentrations of milk sample from permeate. Among the membrane pore sizes tested, it was found that the 10 kDa MWCO membrane cassette can collected more protein compare with 50 kDa MWCO membrane cassette and conventional method. Permeate flux was primarily affected by pump speed and transmembrane pressure. The optimal operating conditions for this separation were transmembrane pressure of 15 psi for 10 kDa MWCO membrane. From the experiment, it showed that the permeate flux increased by increasing the time of operation, and transmembrane pressure. The high of transmembrane pressure can affect the process of protein separation because some fouling occurred at the membrane. |
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