Screening of variables relevant to biomass yield and biopolymer yield in shake-flask fermentation for biopolymer.

The production of biodegradable polymer poly (3-hydroxybutyrate) can be done by the bacteria Cupriavidus necator under depletion of nitrogen source and excess source of carbon. In this research, screening of variables which are involved in the production of PHB was done. The experiment was focusing...

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Main Author:	Nurul Nadiah, Manap
Format:	Undergraduates Project Papers
Language:	English
Published:	2009
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/749/ http://umpir.ump.edu.my/id/eprint/749/ http://umpir.ump.edu.my/id/eprint/749/1/Nurul_Nadiah_Manap.pdf

id	ump-749
recordtype	eprints
spelling	ump-7492015-03-03T07:46:40Z http://umpir.ump.edu.my/id/eprint/749/ Screening of variables relevant to biomass yield and biopolymer yield in shake-flask fermentation for biopolymer. Nurul Nadiah, Manap TP Chemical technology The production of biodegradable polymer poly (3-hydroxybutyrate) can be done by the bacteria Cupriavidus necator under depletion of nitrogen source and excess source of carbon. In this research, screening of variables which are involved in the production of PHB was done. The experiment was focusing on determining which variables do not give significant effect toward the production of PHB. The significant effect for each variable which are the agitation rate, temperature, glucose, peptone, disodium hydrogen phosphate, ammonium sulphate, magnesium sulphate and potassium di-hydrogen phosphate were analyzed by using the factor analysis method.Linear models were used to describe the yield as the dependent variable in terms of factors as the independent variables. Starting with the factor corresponding to the smallest eigenvalues and proceeding with factors corresponding to successively bigger eigenvalues, factors were dropped and the mean square error of the resulting linear model is compared with the mean square error of the full model using the F test. For the linear regression involving uncorrelated variables for biomass yield, concentration of di-sodium phosphate and potassium dihydrogen phosphate respectively gave significant effect toward the yield. In addition, for biopolymer yield, the concentrations of peptone, ammonium sulphate, disodium hydrogen phosphate and potassium dihydrogen phosphate respectively give a significant effect toward the yield. 2009-05 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/749/1/Nurul_Nadiah_Manap.pdf Nurul Nadiah, Manap (2009) Screening of variables relevant to biomass yield and biopolymer yield in shake-flask fermentation for biopolymer. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:45096&theme=UMP2
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Nurul Nadiah, Manap Screening of variables relevant to biomass yield and biopolymer yield in shake-flask fermentation for biopolymer.
description	The production of biodegradable polymer poly (3-hydroxybutyrate) can be done by the bacteria Cupriavidus necator under depletion of nitrogen source and excess source of carbon. In this research, screening of variables which are involved in the production of PHB was done. The experiment was focusing on determining which variables do not give significant effect toward the production of PHB. The significant effect for each variable which are the agitation rate, temperature, glucose, peptone, disodium hydrogen phosphate, ammonium sulphate, magnesium sulphate and potassium di-hydrogen phosphate were analyzed by using the factor analysis method.Linear models were used to describe the yield as the dependent variable in terms of factors as the independent variables. Starting with the factor corresponding to the smallest eigenvalues and proceeding with factors corresponding to successively bigger eigenvalues, factors were dropped and the mean square error of the resulting linear model is compared with the mean square error of the full model using the F test. For the linear regression involving uncorrelated variables for biomass yield, concentration of di-sodium phosphate and potassium dihydrogen phosphate respectively gave significant effect toward the yield. In addition, for biopolymer yield, the concentrations of peptone, ammonium sulphate, disodium hydrogen phosphate and potassium dihydrogen phosphate respectively give a significant effect toward the yield.
format	Undergraduates Project Papers
author	Nurul Nadiah, Manap
author_facet	Nurul Nadiah, Manap
author_sort	Nurul Nadiah, Manap
title	Screening of variables relevant to biomass yield and biopolymer yield in shake-flask fermentation for biopolymer.
title_short	Screening of variables relevant to biomass yield and biopolymer yield in shake-flask fermentation for biopolymer.
title_full	Screening of variables relevant to biomass yield and biopolymer yield in shake-flask fermentation for biopolymer.
title_fullStr	Screening of variables relevant to biomass yield and biopolymer yield in shake-flask fermentation for biopolymer.
title_full_unstemmed	Screening of variables relevant to biomass yield and biopolymer yield in shake-flask fermentation for biopolymer.
title_sort	screening of variables relevant to biomass yield and biopolymer yield in shake-flask fermentation for biopolymer.
publishDate	2009
url	http://umpir.ump.edu.my/id/eprint/749/ http://umpir.ump.edu.my/id/eprint/749/ http://umpir.ump.edu.my/id/eprint/749/1/Nurul_Nadiah_Manap.pdf
first_indexed	2023-09-18T21:53:15Z
last_indexed	2023-09-18T21:53:15Z
_version_	1777413910115123200

Screening of variables relevant to biomass yield and biopolymer yield in shake-flask fermentation for biopolymer.

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