Studies of esterification using Carica papaya lipase

In search for newer esterification method in industry, application of carica papaya lipase (CPL) satisfies that need. CPL is an enzyme that catalyses the esterification and hydrolysis. Thus it is classified as biocatalyst. This lipase is found in the unripe papaya fruit latex. The aim of this study...

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Main Author:	Neshabran , Ramachandran
Format:	Undergraduates Project Papers
Language:	English
Published:	2012
Subjects:	QD Chemistry
Online Access:	http://umpir.ump.edu.my/id/eprint/7814/ http://umpir.ump.edu.my/id/eprint/7814/ http://umpir.ump.edu.my/id/eprint/7814/1/NESHABRAN_AL_RAMACHANDRAN.PDF

id	ump-7814
recordtype	eprints
spelling	ump-78142015-03-03T09:38:15Z http://umpir.ump.edu.my/id/eprint/7814/ Studies of esterification using Carica papaya lipase Neshabran , Ramachandran QD Chemistry In search for newer esterification method in industry, application of carica papaya lipase (CPL) satisfies that need. CPL is an enzyme that catalyses the esterification and hydrolysis. Thus it is classified as biocatalyst. This lipase is found in the unripe papaya fruit latex. The aim of this study is to study the lipolytic activity by using carica papaya lipase. To study this lipolytic activity of CPL, the latex from the unripe papaya fruit is obtained by making longitudinal incision on the fruit and collecting the latex in a beaker then dried to form latex powder. Then a series of four different alcohols and four different carboxylic acids, both ranging from three carbons to six carbons are used to in the esterification reaction. To analyze the esters formed gas chromatography (GCFID) is used. GC can be used to perform both qualitative analysis to identify the ester formed and quantitative analysis to determine the yield. Esterification using different alcohols and different carboxylic acids will hopefully show different efficiency depending on chain length. The results show that CPL can conduct lipolytic activity (esterification) with various alcohols and various carboxylic acids in industry. 2012 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/7814/1/NESHABRAN_AL_RAMACHANDRAN.PDF Neshabran , Ramachandran (2012) Studies of esterification using Carica papaya lipase. Faculty of Industrial Sciences & Technology, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:76315&theme=UMP2
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	QD Chemistry
spellingShingle	QD Chemistry Neshabran , Ramachandran Studies of esterification using Carica papaya lipase
description	In search for newer esterification method in industry, application of carica papaya lipase (CPL) satisfies that need. CPL is an enzyme that catalyses the esterification and hydrolysis. Thus it is classified as biocatalyst. This lipase is found in the unripe papaya fruit latex. The aim of this study is to study the lipolytic activity by using carica papaya lipase. To study this lipolytic activity of CPL, the latex from the unripe papaya fruit is obtained by making longitudinal incision on the fruit and collecting the latex in a beaker then dried to form latex powder. Then a series of four different alcohols and four different carboxylic acids, both ranging from three carbons to six carbons are used to in the esterification reaction. To analyze the esters formed gas chromatography (GCFID) is used. GC can be used to perform both qualitative analysis to identify the ester formed and quantitative analysis to determine the yield. Esterification using different alcohols and different carboxylic acids will hopefully show different efficiency depending on chain length. The results show that CPL can conduct lipolytic activity (esterification) with various alcohols and various carboxylic acids in industry.
format	Undergraduates Project Papers
author	Neshabran , Ramachandran
author_facet	Neshabran , Ramachandran
author_sort	Neshabran , Ramachandran
title	Studies of esterification using Carica papaya lipase
title_short	Studies of esterification using Carica papaya lipase
title_full	Studies of esterification using Carica papaya lipase
title_fullStr	Studies of esterification using Carica papaya lipase
title_full_unstemmed	Studies of esterification using Carica papaya lipase
title_sort	studies of esterification using carica papaya lipase
publishDate	2012
url	http://umpir.ump.edu.my/id/eprint/7814/ http://umpir.ump.edu.my/id/eprint/7814/ http://umpir.ump.edu.my/id/eprint/7814/1/NESHABRAN_AL_RAMACHANDRAN.PDF
first_indexed	2023-09-18T22:04:50Z
last_indexed	2023-09-18T22:04:50Z
_version_	1777414638402535424

Studies of esterification using Carica papaya lipase

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