Cytotoxic activity of different fractions of Tinospora Crispa stems extracts

Cytotoxic activity of different fractions of Tinospora Crispa stems extracts

"Patawali" (Malay) refers to Tinospora crispa (Menispermaceae) which has been claimed to have many traditional uses. The root, stem and leaves of this plant have been used for many medicinal purposes such as fever, diabetes and rheumatism. Tinospora crispa contains variety of phytochemical...

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Main Author: Sasikala, Mahalingam
Format: Undergraduates Project Papers
Language:English
Published: 2012
Subjects:
QK Botany
Online Access:http://umpir.ump.edu.my/id/eprint/7841/
http://umpir.ump.edu.my/id/eprint/7841/
http://umpir.ump.edu.my/id/eprint/7841/1/SASIKALA_AP_MAHALINGAM.PDF
id ump-7841
recordtype eprints
spelling ump-78412015-03-03T09:38:23Z http://umpir.ump.edu.my/id/eprint/7841/ Cytotoxic activity of different fractions of Tinospora Crispa stems extracts Sasikala, Mahalingam QK Botany "Patawali" (Malay) refers to Tinospora crispa (Menispermaceae) which has been claimed to have many traditional uses. The root, stem and leaves of this plant have been used for many medicinal purposes such as fever, diabetes and rheumatism. Tinospora crispa contains variety of phytochemicals. In this study, the powdered Tinospora crispa stems were extracted using different solvents and pH to obtain five fractions (Fraction 1, 2, 3, 4 and 5) with varying polarity. These fractions were then screened for cytoxicity test using brine shrimp lethality assay. For each fraction, 3 concentrations were prepared (10, 100 and 1000 .tg/mL) and the assay was conducted in triplicates. The result obtained showed only Fraction 3 and 4 were toxic with LC 50 value of 41.59 jig/mL and 118.58 ig/mL respectively whereas Fraction 1, 2 and 5 did not exhibited any LC 50 value for the range of concentrations prepared. Brine shrimp lethality assay results from this study may be used as preliminary indication as anticancer agent of the likely presence of toxic compounds in those particular fractions. Other cytotoxicity tests and specific bioassays may be done on the isolated bioactive compounds later. 2012 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/7841/1/SASIKALA_AP_MAHALINGAM.PDF Sasikala, Mahalingam (2012) Cytotoxic activity of different fractions of Tinospora Crispa stems extracts. Faculty of Industrial Sciences & Technology, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:76205&theme=UMP2
repository_type Digital Repository
institution_category Local University
institution Universiti Malaysia Pahang
building UMP Institutional Repository
collection Online Access
language English
topic QK Botany
spellingShingle QK Botany
Sasikala, Mahalingam
Cytotoxic activity of different fractions of Tinospora Crispa stems extracts
description "Patawali" (Malay) refers to Tinospora crispa (Menispermaceae) which has been claimed to have many traditional uses. The root, stem and leaves of this plant have been used for many medicinal purposes such as fever, diabetes and rheumatism. Tinospora crispa contains variety of phytochemicals. In this study, the powdered Tinospora crispa stems were extracted using different solvents and pH to obtain five fractions (Fraction 1, 2, 3, 4 and 5) with varying polarity. These fractions were then screened for cytoxicity test using brine shrimp lethality assay. For each fraction, 3 concentrations were prepared (10, 100 and 1000 .tg/mL) and the assay was conducted in triplicates. The result obtained showed only Fraction 3 and 4 were toxic with LC 50 value of 41.59 jig/mL and 118.58 ig/mL respectively whereas Fraction 1, 2 and 5 did not exhibited any LC 50 value for the range of concentrations prepared. Brine shrimp lethality assay results from this study may be used as preliminary indication as anticancer agent of the likely presence of toxic compounds in those particular fractions. Other cytotoxicity tests and specific bioassays may be done on the isolated bioactive compounds later.
format Undergraduates Project Papers
author Sasikala, Mahalingam
author_facet Sasikala, Mahalingam
author_sort Sasikala, Mahalingam
title Cytotoxic activity of different fractions of Tinospora Crispa stems extracts
title_short Cytotoxic activity of different fractions of Tinospora Crispa stems extracts
title_full Cytotoxic activity of different fractions of Tinospora Crispa stems extracts
title_fullStr Cytotoxic activity of different fractions of Tinospora Crispa stems extracts
title_full_unstemmed Cytotoxic activity of different fractions of Tinospora Crispa stems extracts
title_sort cytotoxic activity of different fractions of tinospora crispa stems extracts
publishDate 2012
url http://umpir.ump.edu.my/id/eprint/7841/
http://umpir.ump.edu.my/id/eprint/7841/
http://umpir.ump.edu.my/id/eprint/7841/1/SASIKALA_AP_MAHALINGAM.PDF
first_indexed 2023-09-18T22:04:53Z
last_indexed 2023-09-18T22:04:53Z
_version_ 1777414642218303488

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