Biochemical Properties of Xylose Reductase Prepared from Adapted Strain of Candida tropicalis
Xylose reductase (XR) is an intracellular enzyme, which catalyzes xylose to xylitol conversion in the microbes. It has potential biotechnological applications in the manufacture of various commercially important specialty bioproducts including xylitol. This study aimed to prepare XR from adapted...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Springer
2014
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| Subjects: | |
| Online Access: | http://umpir.ump.edu.my/id/eprint/7998/ http://umpir.ump.edu.my/id/eprint/7998/ http://umpir.ump.edu.my/id/eprint/7998/ http://umpir.ump.edu.my/id/eprint/7998/1/Biochemical_Properties_of_Xylose_Reductase_Prepared_from_Adapted_Strain_of_Candida_tropicalis.pdf |
| Summary: | Xylose reductase (XR) is an intracellular enzyme, which catalyzes xylose to xylitol
conversion in the microbes. It has potential biotechnological applications in the manufacture of
various commercially important specialty bioproducts including xylitol. This study aimed to
prepare XR from adapted strain of Candida tropicalis and to characterize it. The XR was
isolated from adapted C. tropicalis, cultivated on Meranti wood sawdust hemicellulosic
hydrolysate (MWSHH)-based medium, via ultrasonication, and was characterized based on
enzyme activity, stability, and kinetic parameters. It was specific to NADPH with an activity of
11.16 U/mL. The enzyme was stable at pH 5–7 and temperature of 25–40 °C for 24 h and
retained above 95 % of its original activity after 4 months of storage at −80 °C. The Km of XR
for xylose and NADPH were 81.78 mM and 7.29 μM while the Vmax for them were 178.57
and 12.5 μM/min, respectively. The high Vmax and low Km values of XR for xylose reflect a
highly productive reaction among XR and xylose. MWSHH can be a promising xylose source
for XR preparation from yeast. |
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