In Vitro Degradation Study of Novel HEC/PVA/collagen Nanofibrous Scaffold for Skin Tissue Engineering Applications

The aim of this study was focused on the degradation behavior of electrospun (hydroxyethyl cellulose/ poly(vinyl) alcohol) HEC/PVA and HEC/PVA/collagen nanofibrous scaffolds, as a potential substrates for skin tissue engineering in two biologically related media: phosphate buffered solution (PBS)...

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Main Authors:	Jahir Hussain, Fathima Shahitha, M. M., Yusoff, Farah Hanani, Zulkifli
Format:	Article
Language:	English English
Published:	Elsevier 2014
Subjects:	QD Chemistry
Online Access:	http://umpir.ump.edu.my/id/eprint/8213/ http://umpir.ump.edu.my/id/eprint/8213/ http://umpir.ump.edu.my/id/eprint/8213/ http://umpir.ump.edu.my/id/eprint/8213/1/In%20Vitro%20Degradation%20Study%20Of%20Novel%20Hec-Pva-Collagen%20Nanofibrous%20Scaffold%20for%20Skin%20Tissue%20Engineering%20Applications.pdf http://umpir.ump.edu.my/id/eprint/8213/7/In%20Vitro%20Degradation%20Study%20Of%20Novel%20Hec-Pva-Collagen%20Nanofibrous%20Scaffold%20for%20Skin%20Tissue%20Engineering%20Applications.pdf

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spelling	ump-82132018-10-16T08:25:53Z http://umpir.ump.edu.my/id/eprint/8213/ In Vitro Degradation Study of Novel HEC/PVA/collagen Nanofibrous Scaffold for Skin Tissue Engineering Applications Jahir Hussain, Fathima Shahitha M. M., Yusoff Farah Hanani, Zulkifli QD Chemistry The aim of this study was focused on the degradation behavior of electrospun (hydroxyethyl cellulose/ poly(vinyl) alcohol) HEC/PVA and HEC/PVA/collagen nanofibrous scaffolds, as a potential substrates for skin tissue engineering in two biologically related media: phosphate buffered solution (PBS) and Dulbecco's modified Eagle's medium (DMEM) for 12 weeks incubation period. The scaffolds were characterized at different degradation times by a series of analysis including pH changes of solutions, weight loss, swelling ratio, SEM, ATR-FTIR, DSC, TGA and mechanical properties. The results indicated that HEC/PVA/collagen scaffolds were exhibited slower degradation rate in both medium as compared to HEC/PVA blend nanofibers. All fibers displayed uneven and rough surfaces towards the final week of incubation in both PBS and DMEM solution. As degradation time increased, there were little changes in the chemical structure as determined by FTIR spectra while thermal studies revealed that the melting temperatures and crystallinity of scaffolds were slightly shifted to a lower value. Both HEC/PVA and HEC/PVA/collagen fibers showed significant decrease in Young's modulus and tensile stress over 12 weeks degradation.These results show that these nanofibrous scaffold demonstrate degradation behavior that meets the requirement as potential degradable biomaterials for dermal replacement. Elsevier 2014 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/8213/1/In%20Vitro%20Degradation%20Study%20Of%20Novel%20Hec-Pva-Collagen%20Nanofibrous%20Scaffold%20for%20Skin%20Tissue%20Engineering%20Applications.pdf application/pdf en http://umpir.ump.edu.my/id/eprint/8213/7/In%20Vitro%20Degradation%20Study%20Of%20Novel%20Hec-Pva-Collagen%20Nanofibrous%20Scaffold%20for%20Skin%20Tissue%20Engineering%20Applications.pdf Jahir Hussain, Fathima Shahitha and M. M., Yusoff and Farah Hanani, Zulkifli (2014) In Vitro Degradation Study of Novel HEC/PVA/collagen Nanofibrous Scaffold for Skin Tissue Engineering Applications. Polymer Degradation and Stability, 110. pp. 473-481. ISSN 0141-3910 http://dx.doi.org/10.1016/j.polymdegradstab.2014.10.017 DOI: 10.1016/j.polymdegradstab.2014.10.017
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English English
topic	QD Chemistry
spellingShingle	QD Chemistry Jahir Hussain, Fathima Shahitha M. M., Yusoff Farah Hanani, Zulkifli In Vitro Degradation Study of Novel HEC/PVA/collagen Nanofibrous Scaffold for Skin Tissue Engineering Applications
description	The aim of this study was focused on the degradation behavior of electrospun (hydroxyethyl cellulose/ poly(vinyl) alcohol) HEC/PVA and HEC/PVA/collagen nanofibrous scaffolds, as a potential substrates for skin tissue engineering in two biologically related media: phosphate buffered solution (PBS) and Dulbecco's modified Eagle's medium (DMEM) for 12 weeks incubation period. The scaffolds were characterized at different degradation times by a series of analysis including pH changes of solutions, weight loss, swelling ratio, SEM, ATR-FTIR, DSC, TGA and mechanical properties. The results indicated that HEC/PVA/collagen scaffolds were exhibited slower degradation rate in both medium as compared to HEC/PVA blend nanofibers. All fibers displayed uneven and rough surfaces towards the final week of incubation in both PBS and DMEM solution. As degradation time increased, there were little changes in the chemical structure as determined by FTIR spectra while thermal studies revealed that the melting temperatures and crystallinity of scaffolds were slightly shifted to a lower value. Both HEC/PVA and HEC/PVA/collagen fibers showed significant decrease in Young's modulus and tensile stress over 12 weeks degradation.These results show that these nanofibrous scaffold demonstrate degradation behavior that meets the requirement as potential degradable biomaterials for dermal replacement.
format	Article
author	Jahir Hussain, Fathima Shahitha M. M., Yusoff Farah Hanani, Zulkifli
author_facet	Jahir Hussain, Fathima Shahitha M. M., Yusoff Farah Hanani, Zulkifli
author_sort	Jahir Hussain, Fathima Shahitha
title	In Vitro Degradation Study of Novel HEC/PVA/collagen Nanofibrous Scaffold for Skin Tissue Engineering Applications
title_short	In Vitro Degradation Study of Novel HEC/PVA/collagen Nanofibrous Scaffold for Skin Tissue Engineering Applications
title_full	In Vitro Degradation Study of Novel HEC/PVA/collagen Nanofibrous Scaffold for Skin Tissue Engineering Applications
title_fullStr	In Vitro Degradation Study of Novel HEC/PVA/collagen Nanofibrous Scaffold for Skin Tissue Engineering Applications
title_full_unstemmed	In Vitro Degradation Study of Novel HEC/PVA/collagen Nanofibrous Scaffold for Skin Tissue Engineering Applications
title_sort	in vitro degradation study of novel hec/pva/collagen nanofibrous scaffold for skin tissue engineering applications
publisher	Elsevier
publishDate	2014
url	http://umpir.ump.edu.my/id/eprint/8213/ http://umpir.ump.edu.my/id/eprint/8213/ http://umpir.ump.edu.my/id/eprint/8213/ http://umpir.ump.edu.my/id/eprint/8213/1/In%20Vitro%20Degradation%20Study%20Of%20Novel%20Hec-Pva-Collagen%20Nanofibrous%20Scaffold%20for%20Skin%20Tissue%20Engineering%20Applications.pdf http://umpir.ump.edu.my/id/eprint/8213/7/In%20Vitro%20Degradation%20Study%20Of%20Novel%20Hec-Pva-Collagen%20Nanofibrous%20Scaffold%20for%20Skin%20Tissue%20Engineering%20Applications.pdf
first_indexed	2023-09-18T22:05:33Z
last_indexed	2023-09-18T22:05:33Z
_version_	1777414683574140928

In Vitro Degradation Study of Novel HEC/PVA/collagen Nanofibrous Scaffold for Skin Tissue Engineering Applications

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