Optimization of inducers on expression of recombinant chitinase in Escherichia coli using response surface methodology

The function of chitinase as a chitin digestive enzyme has attracted the attention as one of the potential materials for control of phytopathogenic fungi and insect pests. However, chitinase is uneconomic for commercialized due to the high cost in its production. The development in recombinant DNA t...

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Main Author:	Norheziwan, Hamsah
Format:	Undergraduates Project Papers
Language:	English
Published:	2009
Subjects:	QD Chemistry
Online Access:	http://umpir.ump.edu.my/id/eprint/831/ http://umpir.ump.edu.my/id/eprint/831/ http://umpir.ump.edu.my/id/eprint/831/1/Norheziwan_Hamsah.pdf

id	ump-831
recordtype	eprints
spelling	ump-8312015-03-03T07:47:08Z http://umpir.ump.edu.my/id/eprint/831/ Optimization of inducers on expression of recombinant chitinase in Escherichia coli using response surface methodology Norheziwan, Hamsah QD Chemistry The function of chitinase as a chitin digestive enzyme has attracted the attention as one of the potential materials for control of phytopathogenic fungi and insect pests. However, chitinase is uneconomic for commercialized due to the high cost in its production. The development in recombinant DNA technology has helped to reduce chitinase production cost and enhance its production. The aim of this study is to determine the optimum conditions toward the production of recombinant chitinase in Escherichia coli by manipulating the concentration of inducers (IPTG and lactose). The best concentration of IPTG and lactose as inducers were determined by using the conventional method. Results from conventional method were used for further improvement of optimization by using Response Surface Methodology (RSM). In Response Surface Methodology, the optimization of expression was carried out based on the Central Composite Design (CCD). The effect of the inducers concentration toward the expression level of the recombinant chitinase production were analyzed and optimum values of tested variables for the production of chitinase were 1.19 mM IPTG and 0.98% lactose. Response surface analysis revealed that 0.513 U/ml of chitinase activity was achieved by using the optimized condition. This improved about 13.2-fold compare to initial experiment which produced 0.039 U/ml. This showed that combination between IPTG and lactose has increased the production of recombinant chitinase. 2009-04 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/831/1/Norheziwan_Hamsah.pdf Norheziwan, Hamsah (2009) Optimization of inducers on expression of recombinant chitinase in Escherichia coli using response surface methodology. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:45481&theme=UMP2
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	QD Chemistry
spellingShingle	QD Chemistry Norheziwan, Hamsah Optimization of inducers on expression of recombinant chitinase in Escherichia coli using response surface methodology
description	The function of chitinase as a chitin digestive enzyme has attracted the attention as one of the potential materials for control of phytopathogenic fungi and insect pests. However, chitinase is uneconomic for commercialized due to the high cost in its production. The development in recombinant DNA technology has helped to reduce chitinase production cost and enhance its production. The aim of this study is to determine the optimum conditions toward the production of recombinant chitinase in Escherichia coli by manipulating the concentration of inducers (IPTG and lactose). The best concentration of IPTG and lactose as inducers were determined by using the conventional method. Results from conventional method were used for further improvement of optimization by using Response Surface Methodology (RSM). In Response Surface Methodology, the optimization of expression was carried out based on the Central Composite Design (CCD). The effect of the inducers concentration toward the expression level of the recombinant chitinase production were analyzed and optimum values of tested variables for the production of chitinase were 1.19 mM IPTG and 0.98% lactose. Response surface analysis revealed that 0.513 U/ml of chitinase activity was achieved by using the optimized condition. This improved about 13.2-fold compare to initial experiment which produced 0.039 U/ml. This showed that combination between IPTG and lactose has increased the production of recombinant chitinase.
format	Undergraduates Project Papers
author	Norheziwan, Hamsah
author_facet	Norheziwan, Hamsah
author_sort	Norheziwan, Hamsah
title	Optimization of inducers on expression of recombinant chitinase in Escherichia coli using response surface methodology
title_short	Optimization of inducers on expression of recombinant chitinase in Escherichia coli using response surface methodology
title_full	Optimization of inducers on expression of recombinant chitinase in Escherichia coli using response surface methodology
title_fullStr	Optimization of inducers on expression of recombinant chitinase in Escherichia coli using response surface methodology
title_full_unstemmed	Optimization of inducers on expression of recombinant chitinase in Escherichia coli using response surface methodology
title_sort	optimization of inducers on expression of recombinant chitinase in escherichia coli using response surface methodology
publishDate	2009
url	http://umpir.ump.edu.my/id/eprint/831/ http://umpir.ump.edu.my/id/eprint/831/ http://umpir.ump.edu.my/id/eprint/831/1/Norheziwan_Hamsah.pdf
first_indexed	2023-09-18T21:53:26Z
last_indexed	2023-09-18T21:53:26Z
_version_	1777413921080082432

Optimization of inducers on expression of recombinant chitinase in Escherichia coli using response surface methodology

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