Identification of Fragrance-Related Transcripts from Selected Orchids Using cDNA Representational Difference Analysis (cDNA-RDA) Approach

Volatile components have been identified in hundreds of orchids including species and hybrids, but the biochemical and molecular biological knowledge on the biosynthesis of scent compounds in orchids is still limited. To date, a strategy combining chemical analysis, genomics and bioinformatics is ad...

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Main Authors:	Barudin, Mohd Aiman, Mohd Hairul, Ab. Rahim, Tan, Suat Hian
Format:	Article
Language:	English
Published:	International Journal of Interdisciplinary and Multidisciplinary Studies 2015
Subjects:	Q Science (General)
Online Access:	http://umpir.ump.edu.my/id/eprint/8797/ http://umpir.ump.edu.my/id/eprint/8797/ http://umpir.ump.edu.my/id/eprint/8797/1/Identification%20of%20Fragrance-Related%20Transcripts%20from%20Selected%20Orchids%20Using%20cDNA%20Representational%20Difference%20Analysis%20%28cDNA-RDA%29%20Approach.pdf

id	ump-8797
recordtype	eprints
spelling	ump-87972018-06-29T01:58:04Z http://umpir.ump.edu.my/id/eprint/8797/ Identification of Fragrance-Related Transcripts from Selected Orchids Using cDNA Representational Difference Analysis (cDNA-RDA) Approach Barudin, Mohd Aiman Mohd Hairul, Ab. Rahim Tan, Suat Hian Q Science (General) Volatile components have been identified in hundreds of orchids including species and hybrids, but the biochemical and molecular biological knowledge on the biosynthesis of scent compounds in orchids is still limited. To date, a strategy combining chemical analysis, genomics and bioinformatics is adopted to unravel the scent biosynthetic pathways that are involved in floral scent biosynthesis in orchids. In this study, cDNA-RDA was used to identify fragrance-related transcripts in three selected fragrant-orchids includingVanda Mimi Palmer, Vanda Small Boy Leong and Vandachostylis Sri-Siamin comparison to Vanda Tan Chay Yan, a nonfragrant orchid. This cDNA-RDA approach allows us toisolate cDNA fragments that are presentin selected fragrant orchids but absent in non-fragrant orchid. Double-stranded cDNA of each fragrant orchid was hybridized separately with double-stranded cDNA of the selected non-fragrant orchid and followed by amplification of subtracted fragments. Three rounds of cDNA-RDAwere carried out to enrich difference cDNA fragments.A number of 21 clones of difference products were sequenced and sequence analysis has shown the presence of three different putative fragrance-related transcripts including methionine synthase, S-adenosyl-Lmethionine synthase, and phenylalanine ammonia lyase. All of the identified transcripts might be involved in fragrance biosynthesis of those fragrant orchids International Journal of Interdisciplinary and Multidisciplinary Studies 2015 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/8797/1/Identification%20of%20Fragrance-Related%20Transcripts%20from%20Selected%20Orchids%20Using%20cDNA%20Representational%20Difference%20Analysis%20%28cDNA-RDA%29%20Approach.pdf Barudin, Mohd Aiman and Mohd Hairul, Ab. Rahim and Tan, Suat Hian (2015) Identification of Fragrance-Related Transcripts from Selected Orchids Using cDNA Representational Difference Analysis (cDNA-RDA) Approach. International Journal of Interdisciplinary and Multidisciplinary Studies, 2 (4). pp. 198-207. ISSN 2348 – 0343 http://www.ijims.com/uploads/0ca08b89804dc723a444hairul25.pdf
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Barudin, Mohd Aiman Mohd Hairul, Ab. Rahim Tan, Suat Hian Identification of Fragrance-Related Transcripts from Selected Orchids Using cDNA Representational Difference Analysis (cDNA-RDA) Approach
description	Volatile components have been identified in hundreds of orchids including species and hybrids, but the biochemical and molecular biological knowledge on the biosynthesis of scent compounds in orchids is still limited. To date, a strategy combining chemical analysis, genomics and bioinformatics is adopted to unravel the scent biosynthetic pathways that are involved in floral scent biosynthesis in orchids. In this study, cDNA-RDA was used to identify fragrance-related transcripts in three selected fragrant-orchids includingVanda Mimi Palmer, Vanda Small Boy Leong and Vandachostylis Sri-Siamin comparison to Vanda Tan Chay Yan, a nonfragrant orchid. This cDNA-RDA approach allows us toisolate cDNA fragments that are presentin selected fragrant orchids but absent in non-fragrant orchid. Double-stranded cDNA of each fragrant orchid was hybridized separately with double-stranded cDNA of the selected non-fragrant orchid and followed by amplification of subtracted fragments. Three rounds of cDNA-RDAwere carried out to enrich difference cDNA fragments.A number of 21 clones of difference products were sequenced and sequence analysis has shown the presence of three different putative fragrance-related transcripts including methionine synthase, S-adenosyl-Lmethionine synthase, and phenylalanine ammonia lyase. All of the identified transcripts might be involved in fragrance biosynthesis of those fragrant orchids
format	Article
author	Barudin, Mohd Aiman Mohd Hairul, Ab. Rahim Tan, Suat Hian
author_facet	Barudin, Mohd Aiman Mohd Hairul, Ab. Rahim Tan, Suat Hian
author_sort	Barudin, Mohd Aiman
title	Identification of Fragrance-Related Transcripts from Selected Orchids Using cDNA Representational Difference Analysis (cDNA-RDA) Approach
title_short	Identification of Fragrance-Related Transcripts from Selected Orchids Using cDNA Representational Difference Analysis (cDNA-RDA) Approach
title_full	Identification of Fragrance-Related Transcripts from Selected Orchids Using cDNA Representational Difference Analysis (cDNA-RDA) Approach
title_fullStr	Identification of Fragrance-Related Transcripts from Selected Orchids Using cDNA Representational Difference Analysis (cDNA-RDA) Approach
title_full_unstemmed	Identification of Fragrance-Related Transcripts from Selected Orchids Using cDNA Representational Difference Analysis (cDNA-RDA) Approach
title_sort	identification of fragrance-related transcripts from selected orchids using cdna representational difference analysis (cdna-rda) approach
publisher	International Journal of Interdisciplinary and Multidisciplinary Studies
publishDate	2015
url	http://umpir.ump.edu.my/id/eprint/8797/ http://umpir.ump.edu.my/id/eprint/8797/ http://umpir.ump.edu.my/id/eprint/8797/1/Identification%20of%20Fragrance-Related%20Transcripts%20from%20Selected%20Orchids%20Using%20cDNA%20Representational%20Difference%20Analysis%20%28cDNA-RDA%29%20Approach.pdf
first_indexed	2023-09-18T22:06:46Z
last_indexed	2023-09-18T22:06:46Z
_version_	1777414759945076736

Identification of Fragrance-Related Transcripts from Selected Orchids Using cDNA Representational Difference Analysis (cDNA-RDA) Approach

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