Production of polysaccharides from white mushrooms (pleurotus florida) by submerged culture fermentation (scf)

P.florida is commonly available edible mushroom among different types of oyster mushrooms of the genus Pleurotus. The bioactive compound contain in the P.florida (polysaccharides and protein) have benefit to health include immunomodulatory, anti-cancer, and hypocholesterolemic effect. The objective...

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Main Author:	Fazlin, Masra
Format:	Undergraduates Project Papers
Language:	English
Published:	2014
Subjects:	QP Physiology
Online Access:	http://umpir.ump.edu.my/id/eprint/9064/ http://umpir.ump.edu.my/id/eprint/9064/ http://umpir.ump.edu.my/id/eprint/9064/1/CD8622%20%40%2039.pdf

id	ump-9064
recordtype	eprints
spelling	ump-90642015-10-21T00:08:36Z http://umpir.ump.edu.my/id/eprint/9064/ Production of polysaccharides from white mushrooms (pleurotus florida) by submerged culture fermentation (scf) Fazlin, Masra QP Physiology P.florida is commonly available edible mushroom among different types of oyster mushrooms of the genus Pleurotus. The bioactive compound contain in the P.florida (polysaccharides and protein) have benefit to health include immunomodulatory, anti-cancer, and hypocholesterolemic effect. The objective of this research is to study the production of polysaccharides and biomass from white mushrooms (P. florida) by submerged culture fermentation (SCF) and determination of protein content by using Kjeldahl method. The effect of harvest time on the production of mycelial biomass, exopolysaccharides (EPS) and protein by P.florida was investigated in research. The optimal culture condition was 20 g/L glucose, 4 g/L yeast extract, 0.46 g/L KH2PO4, 1 g/L K2HPO4, and 0.5 g/L MgSO4 7 H2O at temperature 28oC and pH 8. The modes of reactor operation used in this cultivation of mushroom are shake flask and air lift bioreactor and Somogyi-Nelson reagent, Phenol-Sulphuric reagent and 4% Phenol reagent were used during the sample analysis. In this present study, the result implies that the highest biomass, polysaccharides and protein yield production change with the time of cultivation. This indicated that the maximum production of production day in producing biomass was at days 15 (2.464 g/L). However, the production polysaccharides and protein indicates the highest productivity on the same day (days 12) with 8.42 g/L and 28.69 % respectively. It was found that productivity in airlift bioreactor much higher than the productivity in the shake flask. Thus it can be concluded that that harvest time selection is very important factor to obtain maximal fungal production. This may due to the gentle agitation and aeration hydraulic in airlift bioreactor leads to the productivity of mycelia biomass, exopolysaccharides and protein more efficient than in shake flask 2014-01 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/9064/1/CD8622%20%40%2039.pdf Fazlin, Masra (2014) Production of polysaccharides from white mushrooms (pleurotus florida) by submerged culture fermentation (scf). Faculty Of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:84634&theme=UMP2
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	QP Physiology
spellingShingle	QP Physiology Fazlin, Masra Production of polysaccharides from white mushrooms (pleurotus florida) by submerged culture fermentation (scf)
description	P.florida is commonly available edible mushroom among different types of oyster mushrooms of the genus Pleurotus. The bioactive compound contain in the P.florida (polysaccharides and protein) have benefit to health include immunomodulatory, anti-cancer, and hypocholesterolemic effect. The objective of this research is to study the production of polysaccharides and biomass from white mushrooms (P. florida) by submerged culture fermentation (SCF) and determination of protein content by using Kjeldahl method. The effect of harvest time on the production of mycelial biomass, exopolysaccharides (EPS) and protein by P.florida was investigated in research. The optimal culture condition was 20 g/L glucose, 4 g/L yeast extract, 0.46 g/L KH2PO4, 1 g/L K2HPO4, and 0.5 g/L MgSO4 7 H2O at temperature 28oC and pH 8. The modes of reactor operation used in this cultivation of mushroom are shake flask and air lift bioreactor and Somogyi-Nelson reagent, Phenol-Sulphuric reagent and 4% Phenol reagent were used during the sample analysis. In this present study, the result implies that the highest biomass, polysaccharides and protein yield production change with the time of cultivation. This indicated that the maximum production of production day in producing biomass was at days 15 (2.464 g/L). However, the production polysaccharides and protein indicates the highest productivity on the same day (days 12) with 8.42 g/L and 28.69 % respectively. It was found that productivity in airlift bioreactor much higher than the productivity in the shake flask. Thus it can be concluded that that harvest time selection is very important factor to obtain maximal fungal production. This may due to the gentle agitation and aeration hydraulic in airlift bioreactor leads to the productivity of mycelia biomass, exopolysaccharides and protein more efficient than in shake flask
format	Undergraduates Project Papers
author	Fazlin, Masra
author_facet	Fazlin, Masra
author_sort	Fazlin, Masra
title	Production of polysaccharides from white mushrooms (pleurotus florida) by submerged culture fermentation (scf)
title_short	Production of polysaccharides from white mushrooms (pleurotus florida) by submerged culture fermentation (scf)
title_full	Production of polysaccharides from white mushrooms (pleurotus florida) by submerged culture fermentation (scf)
title_fullStr	Production of polysaccharides from white mushrooms (pleurotus florida) by submerged culture fermentation (scf)
title_full_unstemmed	Production of polysaccharides from white mushrooms (pleurotus florida) by submerged culture fermentation (scf)
title_sort	production of polysaccharides from white mushrooms (pleurotus florida) by submerged culture fermentation (scf)
publishDate	2014
url	http://umpir.ump.edu.my/id/eprint/9064/ http://umpir.ump.edu.my/id/eprint/9064/ http://umpir.ump.edu.my/id/eprint/9064/1/CD8622%20%40%2039.pdf
first_indexed	2023-09-18T22:07:14Z
last_indexed	2023-09-18T22:07:14Z
_version_	1777414789361827840

Production of polysaccharides from white mushrooms (pleurotus florida) by submerged culture fermentation (scf)

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