Sequence Analysis of New Isolated O-methyltransferase Transcript (VMPOMT) from Vanda Mimi Palmer

Vanda Mimi Palmer is an orchid hybrid between Vanda Tan Chay Yan and Vanda tessellata. This orchid has won several international awards for its fragrance emission characteristic. To date, scientific studies on enzymes as well as their related genes and transcripts that are involved in floral scent p...

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Main Authors:	Barudin, Mohd Aiman, Mohd Hairul, Ab. Rahim, Tan, Suat Hian
Format:	Article
Language:	English
Published:	Scholars Academic and Scientific Publisher 2015
Subjects:	Q Science (General)
Online Access:	http://umpir.ump.edu.my/id/eprint/9125/ http://umpir.ump.edu.my/id/eprint/9125/ http://umpir.ump.edu.my/id/eprint/9125/1/Sequence%20Analysis%20of%20New%20Isolated%20O-methyltransferase%20Transcript%20%28VMPOMT%29%20from%20Vanda%20Mimi%20Palmer.pdf

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spelling	ump-91252018-06-29T03:57:45Z http://umpir.ump.edu.my/id/eprint/9125/ Sequence Analysis of New Isolated O-methyltransferase Transcript (VMPOMT) from Vanda Mimi Palmer Barudin, Mohd Aiman Mohd Hairul, Ab. Rahim Tan, Suat Hian Q Science (General) Vanda Mimi Palmer is an orchid hybrid between Vanda Tan Chay Yan and Vanda tessellata. This orchid has won several international awards for its fragrance emission characteristic. To date, scientific studies on enzymes as well as their related genes and transcripts that are involved in floral scent production and biosynthesis are barely covered in fragrant orchids especially in vandaceous orchids. The aims of this study were to isolate full open reading frame (ORF) of O-methyltransferase transcript from Vanda Mimi Palmer (VMPOMT) and followed by sequence characterisation using bioinformatics tools. The size of successfully isolated full-length transcript of VMPOMT (Genbank accession no: KF278721) is 1,289bp, encoding for a polypeptide of 368 amino acid residues consisting of 1,104bp open reading frame (ORF) flanked by 2bp of 5'-untranslated region (UTR) and 182bp of 3'-UTR including a poly-A tail. Sequence analyses using bioinformatics tools have shown that VMPOMT has a conserved motif for O-methyltransferase group as found in OMT sequences from other plants that might be involved in methylation reactions in fragrance compounds biosynthesis in this orchid. Besides that, VMPOMT is predicted to be localised in cytoplasm instead of plastid due to the absence of chloroplast transit peptide in the sequence. Thus, sequence analyses on VMPOMT using several bioinformatics tools might contribute to the understanding of fragrance biosynthetic pathways in Vanda Mimi Palmer as well as in other fragrant orchids Scholars Academic and Scientific Publisher 2015 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/9125/1/Sequence%20Analysis%20of%20New%20Isolated%20O-methyltransferase%20Transcript%20%28VMPOMT%29%20from%20Vanda%20Mimi%20Palmer.pdf Barudin, Mohd Aiman and Mohd Hairul, Ab. Rahim and Tan, Suat Hian (2015) Sequence Analysis of New Isolated O-methyltransferase Transcript (VMPOMT) from Vanda Mimi Palmer. Scholars Academic Journal of Biosciences (SAJB), 3 (3). pp. 239-247. ISSN 2321-6883 (Online); 2347-9515 (Print) http://saspublisher.com/wp-content/uploads/2015/03/SAJB-33239-247.pdf
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Barudin, Mohd Aiman Mohd Hairul, Ab. Rahim Tan, Suat Hian Sequence Analysis of New Isolated O-methyltransferase Transcript (VMPOMT) from Vanda Mimi Palmer
description	Vanda Mimi Palmer is an orchid hybrid between Vanda Tan Chay Yan and Vanda tessellata. This orchid has won several international awards for its fragrance emission characteristic. To date, scientific studies on enzymes as well as their related genes and transcripts that are involved in floral scent production and biosynthesis are barely covered in fragrant orchids especially in vandaceous orchids. The aims of this study were to isolate full open reading frame (ORF) of O-methyltransferase transcript from Vanda Mimi Palmer (VMPOMT) and followed by sequence characterisation using bioinformatics tools. The size of successfully isolated full-length transcript of VMPOMT (Genbank accession no: KF278721) is 1,289bp, encoding for a polypeptide of 368 amino acid residues consisting of 1,104bp open reading frame (ORF) flanked by 2bp of 5'-untranslated region (UTR) and 182bp of 3'-UTR including a poly-A tail. Sequence analyses using bioinformatics tools have shown that VMPOMT has a conserved motif for O-methyltransferase group as found in OMT sequences from other plants that might be involved in methylation reactions in fragrance compounds biosynthesis in this orchid. Besides that, VMPOMT is predicted to be localised in cytoplasm instead of plastid due to the absence of chloroplast transit peptide in the sequence. Thus, sequence analyses on VMPOMT using several bioinformatics tools might contribute to the understanding of fragrance biosynthetic pathways in Vanda Mimi Palmer as well as in other fragrant orchids
format	Article
author	Barudin, Mohd Aiman Mohd Hairul, Ab. Rahim Tan, Suat Hian
author_facet	Barudin, Mohd Aiman Mohd Hairul, Ab. Rahim Tan, Suat Hian
author_sort	Barudin, Mohd Aiman
title	Sequence Analysis of New Isolated O-methyltransferase Transcript (VMPOMT) from Vanda Mimi Palmer
title_short	Sequence Analysis of New Isolated O-methyltransferase Transcript (VMPOMT) from Vanda Mimi Palmer
title_full	Sequence Analysis of New Isolated O-methyltransferase Transcript (VMPOMT) from Vanda Mimi Palmer
title_fullStr	Sequence Analysis of New Isolated O-methyltransferase Transcript (VMPOMT) from Vanda Mimi Palmer
title_full_unstemmed	Sequence Analysis of New Isolated O-methyltransferase Transcript (VMPOMT) from Vanda Mimi Palmer
title_sort	sequence analysis of new isolated o-methyltransferase transcript (vmpomt) from vanda mimi palmer
publisher	Scholars Academic and Scientific Publisher
publishDate	2015
url	http://umpir.ump.edu.my/id/eprint/9125/ http://umpir.ump.edu.my/id/eprint/9125/ http://umpir.ump.edu.my/id/eprint/9125/1/Sequence%20Analysis%20of%20New%20Isolated%20O-methyltransferase%20Transcript%20%28VMPOMT%29%20from%20Vanda%20Mimi%20Palmer.pdf
first_indexed	2023-09-18T22:07:21Z
last_indexed	2023-09-18T22:07:21Z
_version_	1777414797487243264

Sequence Analysis of New Isolated O-methyltransferase Transcript (VMPOMT) from Vanda Mimi Palmer

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