Production of poly (3-hydroxybutyrate) from pineapple waste

P(3HB) is the potential replacement of synthetic plastics due to its biodegradability and sustainability. Pineapple waste is one of the example of food waste that capable to produce P(3HB) as they contains sucrose, glucose, fructose and other nutrients that essential for production of bio plastics....

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Main Author:	Nur Amira, Maarof
Format:	Undergraduates Project Papers
Language:	English
Published:	2014
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/9158/ http://umpir.ump.edu.my/id/eprint/9158/ http://umpir.ump.edu.my/id/eprint/9158/1/cd8659.pdf

id	ump-9158
recordtype	eprints
spelling	ump-91582015-10-16T00:05:18Z http://umpir.ump.edu.my/id/eprint/9158/ Production of poly (3-hydroxybutyrate) from pineapple waste Nur Amira, Maarof TP Chemical technology P(3HB) is the potential replacement of synthetic plastics due to its biodegradability and sustainability. Pineapple waste is one of the example of food waste that capable to produce P(3HB) as they contains sucrose, glucose, fructose and other nutrients that essential for production of bio plastics. Thus, the aim of this research is to employ the sugar present in pineapple waste as the carbon source for the bacteria producing biopolymer. The objectives are as follow; to determine the sugar content in the pineapple waste juice, to study the effect percent volume of juice from pineapple waste on production of P(3HB), to study the effect of pH on P(3HB) production. The method involve in this study are cultivation of bacteria, preparation of pineapple waste, preparation of inoculum, P(3HB) biosynthesis in shake flask, cell dry weight analysis and analysis of P(3HB) using HPLC. The results showed that glucose content in the mixture of skins and pulps is the highest with total of 28.80 g/L compared to fructose and sucrose. Fructose content come second with 21.25 g/L while sucrose has the lowest content in the PWJ with 20.28 g/L. The pineapple waste juice (PWJ) is then used as medium for the production of P(3HB). The fermentation medium with 50% and 100% volume of PWJ has the highest concentration of P(3HB) of 2.01 g/L (30th hour) and 1.49 g/L (44th hour) respectively. As the total dry mass production, the medium with 75% of PWJ has the highest concentration of P(3HB) with 14.42 g/L. The total concentration of P(3HB) for 50% and 100% PWJ are 10.52 g/L and 4.42 g/L respectively. The total average concentration of P(3HB) produced in the medium at pH 7 is 8.97 g/L. The highest concentration was found to be at the 44th hours with 2.15 g/L. As conclusion, the medium that consists of 75% PWJ and 25% ultra-pure water with the mixture of nutrient rich element at pH 7 is the most suitable medium to produce P(3HB). 2014-06 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/9158/1/cd8659.pdf Nur Amira, Maarof (2014) Production of poly (3-hydroxybutyrate) from pineapple waste. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:84596&theme=UMP2
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Nur Amira, Maarof Production of poly (3-hydroxybutyrate) from pineapple waste
description	P(3HB) is the potential replacement of synthetic plastics due to its biodegradability and sustainability. Pineapple waste is one of the example of food waste that capable to produce P(3HB) as they contains sucrose, glucose, fructose and other nutrients that essential for production of bio plastics. Thus, the aim of this research is to employ the sugar present in pineapple waste as the carbon source for the bacteria producing biopolymer. The objectives are as follow; to determine the sugar content in the pineapple waste juice, to study the effect percent volume of juice from pineapple waste on production of P(3HB), to study the effect of pH on P(3HB) production. The method involve in this study are cultivation of bacteria, preparation of pineapple waste, preparation of inoculum, P(3HB) biosynthesis in shake flask, cell dry weight analysis and analysis of P(3HB) using HPLC. The results showed that glucose content in the mixture of skins and pulps is the highest with total of 28.80 g/L compared to fructose and sucrose. Fructose content come second with 21.25 g/L while sucrose has the lowest content in the PWJ with 20.28 g/L. The pineapple waste juice (PWJ) is then used as medium for the production of P(3HB). The fermentation medium with 50% and 100% volume of PWJ has the highest concentration of P(3HB) of 2.01 g/L (30th hour) and 1.49 g/L (44th hour) respectively. As the total dry mass production, the medium with 75% of PWJ has the highest concentration of P(3HB) with 14.42 g/L. The total concentration of P(3HB) for 50% and 100% PWJ are 10.52 g/L and 4.42 g/L respectively. The total average concentration of P(3HB) produced in the medium at pH 7 is 8.97 g/L. The highest concentration was found to be at the 44th hours with 2.15 g/L. As conclusion, the medium that consists of 75% PWJ and 25% ultra-pure water with the mixture of nutrient rich element at pH 7 is the most suitable medium to produce P(3HB).
format	Undergraduates Project Papers
author	Nur Amira, Maarof
author_facet	Nur Amira, Maarof
author_sort	Nur Amira, Maarof
title	Production of poly (3-hydroxybutyrate) from pineapple waste
title_short	Production of poly (3-hydroxybutyrate) from pineapple waste
title_full	Production of poly (3-hydroxybutyrate) from pineapple waste
title_fullStr	Production of poly (3-hydroxybutyrate) from pineapple waste
title_full_unstemmed	Production of poly (3-hydroxybutyrate) from pineapple waste
title_sort	production of poly (3-hydroxybutyrate) from pineapple waste
publishDate	2014
url	http://umpir.ump.edu.my/id/eprint/9158/ http://umpir.ump.edu.my/id/eprint/9158/ http://umpir.ump.edu.my/id/eprint/9158/1/cd8659.pdf
first_indexed	2023-09-18T22:07:26Z
last_indexed	2023-09-18T22:07:26Z
_version_	1777414802062180352

Production of poly (3-hydroxybutyrate) from pineapple waste

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