Statistical Optimization of Green Fluorescent Protein Production from Escherichia coli BL21(DE3)

An optimized cultivation condition is needed to maximize the functional green fluorescent protein (GFP) production. Six process variables (agitation rate, temperature, initial medium pH, concentration of inducer, time of induction, and inoculum density) were screened using the fractional factorial d...

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Main Authors:	Chew, Few Ne, Tan, Siang Wen, Boo, Chern Huey, Tey, Ti Beng
Format:	Article
Published:	Taylor & Francis Group 2012
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/9310/ http://umpir.ump.edu.my/id/eprint/9310/ http://umpir.ump.edu.my/id/eprint/9310/

id	ump-9310
recordtype	eprints
spelling	ump-93102018-05-14T06:41:59Z http://umpir.ump.edu.my/id/eprint/9310/ Statistical Optimization of Green Fluorescent Protein Production from Escherichia coli BL21(DE3) Chew, Few Ne Tan, Siang Wen Boo, Chern Huey Tey, Ti Beng TP Chemical technology An optimized cultivation condition is needed to maximize the functional green fluorescent protein (GFP) production. Six process variables (agitation rate, temperature, initial medium pH, concentration of inducer, time of induction, and inoculum density) were screened using the fractional factorial design. Three variables (agitation rate, temperature, and time of induction) exerted significant effects on functional GFP production in E. coli shake flask cultivation and were optimized subsequently using the Box-Behnken design. An agitation rate of 206 rpm at 31°C and induction of the protein expression when the cell density (OD(600nm)) reaches 1.04 could enhance the yield of functional GFP production from 0.025 g/L to 0.241 g/L, which is about ninefold higher than the unoptimized conditions. Unoptimized cultivation conditions resulted in protein aggregation and hence reduced the quantity of functional GFP. The model and regression equation based on the shake flask cultivation could be applied to a 2-L bioreactor for maximum functional GFP production. Taylor & Francis Group 2012 Article PeerReviewed Chew, Few Ne and Tan, Siang Wen and Boo, Chern Huey and Tey, Ti Beng (2012) Statistical Optimization of Green Fluorescent Protein Production from Escherichia coli BL21(DE3). Preparative Biochemistry and Biotechnology, 42 (6). pp. 535-550. ISSN 1082-6068 http://dx.doi.org/10.1080/10826068.2012.660903 DOI: Taylor & Francis Group, Taylor & Francis Group
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
topic	TP Chemical technology
spellingShingle	TP Chemical technology Chew, Few Ne Tan, Siang Wen Boo, Chern Huey Tey, Ti Beng Statistical Optimization of Green Fluorescent Protein Production from Escherichia coli BL21(DE3)
description	An optimized cultivation condition is needed to maximize the functional green fluorescent protein (GFP) production. Six process variables (agitation rate, temperature, initial medium pH, concentration of inducer, time of induction, and inoculum density) were screened using the fractional factorial design. Three variables (agitation rate, temperature, and time of induction) exerted significant effects on functional GFP production in E. coli shake flask cultivation and were optimized subsequently using the Box-Behnken design. An agitation rate of 206 rpm at 31°C and induction of the protein expression when the cell density (OD(600nm)) reaches 1.04 could enhance the yield of functional GFP production from 0.025 g/L to 0.241 g/L, which is about ninefold higher than the unoptimized conditions. Unoptimized cultivation conditions resulted in protein aggregation and hence reduced the quantity of functional GFP. The model and regression equation based on the shake flask cultivation could be applied to a 2-L bioreactor for maximum functional GFP production.
format	Article
author	Chew, Few Ne Tan, Siang Wen Boo, Chern Huey Tey, Ti Beng
author_facet	Chew, Few Ne Tan, Siang Wen Boo, Chern Huey Tey, Ti Beng
author_sort	Chew, Few Ne
title	Statistical Optimization of Green Fluorescent Protein Production from Escherichia coli BL21(DE3)
title_short	Statistical Optimization of Green Fluorescent Protein Production from Escherichia coli BL21(DE3)
title_full	Statistical Optimization of Green Fluorescent Protein Production from Escherichia coli BL21(DE3)
title_fullStr	Statistical Optimization of Green Fluorescent Protein Production from Escherichia coli BL21(DE3)
title_full_unstemmed	Statistical Optimization of Green Fluorescent Protein Production from Escherichia coli BL21(DE3)
title_sort	statistical optimization of green fluorescent protein production from escherichia coli bl21(de3)
publisher	Taylor & Francis Group
publishDate	2012
url	http://umpir.ump.edu.my/id/eprint/9310/ http://umpir.ump.edu.my/id/eprint/9310/ http://umpir.ump.edu.my/id/eprint/9310/
first_indexed	2023-09-18T22:07:45Z
last_indexed	2023-09-18T22:07:45Z
_version_	1777414822429720576

Statistical Optimization of Green Fluorescent Protein Production from Escherichia coli BL21(DE3)

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