Bacterial Isolation from Palm Oil Plantation Soil for Biodiesel Production: Isolation and Molecular Identification as Inferred by 16s RNA

Biodiesel (methyl esters) is a clean alternative fuel which can be produced from many renewable resources. Palm oil, like other vegetable oils, can be used as feedstock for biodiesel production through transesterification to produce palm oil methyl ester. Various microorganisms like bacteria and fun...

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Main Authors:	Meng, Liu, M. M., Yusoff, Makky, Essam A., Jailani, Salihon
Format:	Article
Language:	English
Published:	JBTBM 2014
Subjects:	Q Science (General)
Online Access:	http://umpir.ump.edu.my/id/eprint/9538/ http://umpir.ump.edu.my/id/eprint/9538/ http://umpir.ump.edu.my/id/eprint/9538/1/Bacterial%20Isolation%20from%20Palm%20Oil%20Plantation%20Soil%20for%20Biodiesel%20Production-%20Isolation%20and%20Molecular%20Identification%20as%20Inferred%20by%2016s%20RNA.pdf

id	ump-9538
recordtype	eprints
spelling	ump-95382018-09-27T08:50:46Z http://umpir.ump.edu.my/id/eprint/9538/ Bacterial Isolation from Palm Oil Plantation Soil for Biodiesel Production: Isolation and Molecular Identification as Inferred by 16s RNA Meng, Liu M. M., Yusoff Makky, Essam A. Jailani, Salihon Q Science (General) Biodiesel (methyl esters) is a clean alternative fuel which can be produced from many renewable resources. Palm oil, like other vegetable oils, can be used as feedstock for biodiesel production through transesterification to produce palm oil methyl ester. Various microorganisms like bacteria and fungi have a diversity application which could be used as catalysts in a series of degradation reactions, such as transesterification. Malaysia is rich in palm oil and therefore, lots of bacteria surviving by consuming palm oil residue resource in palm oil plantation. In this study, eighteen (18) bacterial strains were successfully isolated from local soil samples and some of their characteristics determined. The optimum temperatures of all strains were in the range of 30 to 37°C, and the optimum batch culture times of all strains were in the range of 24 to 48 hours. All strains were submitted for Gram-staining. Three (3) strains denominated as A, B and C that was involved in the most significant transesterification reaction was selected for identification by submitting them to biochemical tests using the commercial API kit. The same three (3) isolates were submitted to identification by molecular technique. Two bacteria were identified to be Pseudomonas geniculata (A) and Stenotrophomonas maltoplilia (C), while the second bacteria (B) identified to be Bacillus pseudomycoides B-60. JBTBM 2014 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/9538/1/Bacterial%20Isolation%20from%20Palm%20Oil%20Plantation%20Soil%20for%20Biodiesel%20Production-%20Isolation%20and%20Molecular%20Identification%20as%20Inferred%20by%2016s%20RNA.pdf Meng, Liu and M. M., Yusoff and Makky, Essam A. and Jailani, Salihon (2014) Bacterial Isolation from Palm Oil Plantation Soil for Biodiesel Production: Isolation and Molecular Identification as Inferred by 16s RNA. Biotechnology & Biomaterials, 4 (1). pp. 1-7. ISSN 2155-952X http://dx.doi.org/10.4172/2155-952X.1000165
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Meng, Liu M. M., Yusoff Makky, Essam A. Jailani, Salihon Bacterial Isolation from Palm Oil Plantation Soil for Biodiesel Production: Isolation and Molecular Identification as Inferred by 16s RNA
description	Biodiesel (methyl esters) is a clean alternative fuel which can be produced from many renewable resources. Palm oil, like other vegetable oils, can be used as feedstock for biodiesel production through transesterification to produce palm oil methyl ester. Various microorganisms like bacteria and fungi have a diversity application which could be used as catalysts in a series of degradation reactions, such as transesterification. Malaysia is rich in palm oil and therefore, lots of bacteria surviving by consuming palm oil residue resource in palm oil plantation. In this study, eighteen (18) bacterial strains were successfully isolated from local soil samples and some of their characteristics determined. The optimum temperatures of all strains were in the range of 30 to 37°C, and the optimum batch culture times of all strains were in the range of 24 to 48 hours. All strains were submitted for Gram-staining. Three (3) strains denominated as A, B and C that was involved in the most significant transesterification reaction was selected for identification by submitting them to biochemical tests using the commercial API kit. The same three (3) isolates were submitted to identification by molecular technique. Two bacteria were identified to be Pseudomonas geniculata (A) and Stenotrophomonas maltoplilia (C), while the second bacteria (B) identified to be Bacillus pseudomycoides B-60.
format	Article
author	Meng, Liu M. M., Yusoff Makky, Essam A. Jailani, Salihon
author_facet	Meng, Liu M. M., Yusoff Makky, Essam A. Jailani, Salihon
author_sort	Meng, Liu
title	Bacterial Isolation from Palm Oil Plantation Soil for Biodiesel Production: Isolation and Molecular Identification as Inferred by 16s RNA
title_short	Bacterial Isolation from Palm Oil Plantation Soil for Biodiesel Production: Isolation and Molecular Identification as Inferred by 16s RNA
title_full	Bacterial Isolation from Palm Oil Plantation Soil for Biodiesel Production: Isolation and Molecular Identification as Inferred by 16s RNA
title_fullStr	Bacterial Isolation from Palm Oil Plantation Soil for Biodiesel Production: Isolation and Molecular Identification as Inferred by 16s RNA
title_full_unstemmed	Bacterial Isolation from Palm Oil Plantation Soil for Biodiesel Production: Isolation and Molecular Identification as Inferred by 16s RNA
title_sort	bacterial isolation from palm oil plantation soil for biodiesel production: isolation and molecular identification as inferred by 16s rna
publisher	JBTBM
publishDate	2014
url	http://umpir.ump.edu.my/id/eprint/9538/ http://umpir.ump.edu.my/id/eprint/9538/ http://umpir.ump.edu.my/id/eprint/9538/1/Bacterial%20Isolation%20from%20Palm%20Oil%20Plantation%20Soil%20for%20Biodiesel%20Production-%20Isolation%20and%20Molecular%20Identification%20as%20Inferred%20by%2016s%20RNA.pdf
first_indexed	2023-09-18T22:08:12Z
last_indexed	2023-09-18T22:08:12Z
_version_	1777414850447671296

Bacterial Isolation from Palm Oil Plantation Soil for Biodiesel Production: Isolation and Molecular Identification as Inferred by 16s RNA

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