Molecular cloning of phytase gene from ASUIA279 and its expression in Pichia Pastoris system

Phytase gene obtained from Bacillus subtilis ASUIA243 was cloned into a medium vector and transformed into E. coli. Restriction enzyme digestion was conducted to get blunt-ended phytase gene and ligated into the Pichia expression vector, pPICZαA. The recombinant vector, pPICZαA-243HPp was then li...

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Bibliographic Details
Main Authors: Maifiah, M.H. Mahamad, Jamal, Parveen, Nuge, Tamrin, Mohd Dali, N.S., Meor Hussin, A.S., Farouk, Abd Alaziem, Mohd. Salleh, Hamzah
Format: Article
Language:English
Published: IIUM Press 2011
Subjects:
Online Access:http://irep.iium.edu.my/17046/
http://irep.iium.edu.my/17046/
http://irep.iium.edu.my/17046/4/219-1318-1-PB.pdf