Direct purification of pectinase from Mango (Mangifera Indica Cv. Chokanan) peel using a PEG/Salt-based aqueous two phase system
An Aqueous Two-Phase System (ATPS) was employed for the first time for the separation and purification of pectinase from mango (Mangifera Indica Cv. Chokanan)peel. The effects of different parameters such as molecular weight of the polymer (polyethylene glycol, 2,000–10,000), potassium phosphate com...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Molecular Diversity Preservation International (MDPI)
2011
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/17926/ http://irep.iium.edu.my/17926/ http://irep.iium.edu.my/17926/ http://irep.iium.edu.my/17926/1/Molecules-2011b.pdf |
| id |
iium-17926 |
|---|---|
| recordtype |
eprints |
| spelling |
iium-179262012-02-21T03:16:20Z http://irep.iium.edu.my/17926/ Direct purification of pectinase from Mango (Mangifera Indica Cv. Chokanan) peel using a PEG/Salt-based aqueous two phase system Mehrnoush, Amid Sarker, Md. Zaidul Islam Mustafa, Shuhaimi Mohd Yazid, Abdul Manap QD Chemistry An Aqueous Two-Phase System (ATPS) was employed for the first time for the separation and purification of pectinase from mango (Mangifera Indica Cv. Chokanan)peel. The effects of different parameters such as molecular weight of the polymer (polyethylene glycol, 2,000–10,000), potassium phosphate composition (12–20%, w/w),system pH (6–9), and addition of different concentrations of neutral salts (0–8%, w/w) on partition behavior of pectinase were investigated. The partition coefficient of the enzyme was decreased by increasing the PEG molecular weight. Additionally, the phase composition showed a significant effect on purification factor and yield of the enzyme. Optimum conditions for purification of pectinase from mango peel were achieved in a 14% PEG 4000-14% potassium phosphate system using 3% (w/w) NaCl addition at pH 7.0. Based on this system, the purification factor of pectinase was increased to 13.2 with a high yield of (97.6%). Thus, this study proves that ATPS can be an inexpensive and effective method for partitioning of pectinase from mango peel. Molecular Diversity Preservation International (MDPI) 2011-10-10 Article PeerReviewed application/pdf en http://irep.iium.edu.my/17926/1/Molecules-2011b.pdf Mehrnoush, Amid and Sarker, Md. Zaidul Islam and Mustafa, Shuhaimi and Mohd Yazid, Abdul Manap (2011) Direct purification of pectinase from Mango (Mangifera Indica Cv. Chokanan) peel using a PEG/Salt-based aqueous two phase system. Molecules , 16 (10). pp. 8419-8427. ISSN 1420-3049 http://www.mdpi.com/1420-3049/16/10/8419/ DOI:10.3390/molecules16108419 |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
International Islamic University Malaysia |
| building |
IIUM Repository |
| collection |
Online Access |
| language |
English |
| topic |
QD Chemistry |
| spellingShingle |
QD Chemistry Mehrnoush, Amid Sarker, Md. Zaidul Islam Mustafa, Shuhaimi Mohd Yazid, Abdul Manap Direct purification of pectinase from Mango (Mangifera Indica Cv. Chokanan) peel using a PEG/Salt-based aqueous two phase system |
| description |
An Aqueous Two-Phase System (ATPS) was employed for the first time for the separation and purification of pectinase from mango (Mangifera Indica Cv. Chokanan)peel. The effects of different parameters such as molecular weight of the polymer (polyethylene glycol, 2,000–10,000), potassium phosphate composition (12–20%, w/w),system pH (6–9), and addition of different concentrations of neutral salts (0–8%, w/w) on partition behavior of pectinase were investigated. The partition coefficient of the enzyme
was decreased by increasing the PEG molecular weight. Additionally, the phase composition showed a significant effect on purification factor and yield of the enzyme. Optimum conditions for purification of pectinase from mango peel were achieved in a 14% PEG 4000-14% potassium phosphate system using 3% (w/w) NaCl addition at pH 7.0. Based on this system, the purification factor of pectinase was increased to 13.2 with a high yield of (97.6%). Thus, this study proves that ATPS can be an inexpensive and effective method for partitioning of pectinase from mango peel. |
| format |
Article |
| author |
Mehrnoush, Amid Sarker, Md. Zaidul Islam Mustafa, Shuhaimi Mohd Yazid, Abdul Manap |
| author_facet |
Mehrnoush, Amid Sarker, Md. Zaidul Islam Mustafa, Shuhaimi Mohd Yazid, Abdul Manap |
| author_sort |
Mehrnoush, Amid |
| title |
Direct purification of pectinase from Mango (Mangifera Indica
Cv. Chokanan) peel using a PEG/Salt-based aqueous two phase system |
| title_short |
Direct purification of pectinase from Mango (Mangifera Indica
Cv. Chokanan) peel using a PEG/Salt-based aqueous two phase system |
| title_full |
Direct purification of pectinase from Mango (Mangifera Indica
Cv. Chokanan) peel using a PEG/Salt-based aqueous two phase system |
| title_fullStr |
Direct purification of pectinase from Mango (Mangifera Indica
Cv. Chokanan) peel using a PEG/Salt-based aqueous two phase system |
| title_full_unstemmed |
Direct purification of pectinase from Mango (Mangifera Indica
Cv. Chokanan) peel using a PEG/Salt-based aqueous two phase system |
| title_sort |
direct purification of pectinase from mango (mangifera indica
cv. chokanan) peel using a peg/salt-based aqueous two phase system |
| publisher |
Molecular Diversity Preservation International (MDPI) |
| publishDate |
2011 |
| url |
http://irep.iium.edu.my/17926/ http://irep.iium.edu.my/17926/ http://irep.iium.edu.my/17926/ http://irep.iium.edu.my/17926/1/Molecules-2011b.pdf |
| first_indexed |
2023-09-18T20:26:58Z |
| last_indexed |
2023-09-18T20:26:58Z |
| _version_ |
1777408481782923264 |