Comparison of different cell disruption methods and cell extractant buffers for recombinant bromelain expressed in E.coli BL21-A1
The often-encountered problem such as protein degradation has driven various methods of cell lysis in obtaining recombinant protein post fermentation. In this paper, we compare methods such as homogenization, sonication, sonication with lysozyme and chemical lysis using B-PER reagent with lysozym...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Universiti Teknologi Malaysia
2015
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/46349/ http://irep.iium.edu.my/46349/ http://irep.iium.edu.my/46349/1/Cell_disruption.pdf |