Process development for maximum Lycopene production from selected fruit waste
Lycopene, one of the most widely used carotenoid is an efficient antioxidant and singlet oxygen quencher. The increasing demand of lycopene in the nutraceutical and drug industry has directed the researchers to produce lycopene with cost effective methods in a large scale to meet the growing dema...
| Main Authors: | , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2015
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/47553/ http://irep.iium.edu.my/47553/ http://irep.iium.edu.my/47553/2/acb_conf2_%28IqPar%29.pdf |
| Summary: | Lycopene, one of the most widely used carotenoid is an efficient antioxidant and singlet
oxygen quencher. The increasing demand of lycopene in the nutraceutical and drug
industry has directed the researchers to produce lycopene with cost effective methods
in a large scale to meet the growing demand. Thermal processing liberates this
carotenoid from complexes with proteins, thus increasing its bioaccessibilty. The
comparison of lycopene content was evaluated amongst fruit peels of guava, papaya,
watermelon and red dragon fruit, in order to choose the best source. Lycopene content
was measured using both UV–vis spectrophotometer and identified using high
performance liquid chromatography (HPLC). Papaya, a tropical fruit showed
tremendous potential as an alternative source and was selected to conduct further
investigation. Response surface methodology (RSM) using faced centered central
composite design (FCCCD) was applied to study the interaction between the most
contributing factors i.e. temperature, time and solid-solvent ratio. From the results,
themaximum lycopene yield of 103.1 mg/kg at temperature (1200C), time (5 hours) in a
solid-solvent ratio of 1:40 g/mL were selected. The lycopene oleoresin was further
purified by saponification and identified using HPLC, thus revealing that the major
constituents of the lycopene oleoresin after saponification were lycopene and β-
carotene which constitute 69.879% and 30.12% of the total oleoresin respectively. |
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