Optimizing Bioethanol Production from Sago Starch in Bioreactor for Renewable Energy

The two-step enzymatic hydrolysis of sago starch was performed by commercially available α-amylase and β- glucoamylase and further ethanol fermentation of the obtained hydrolyzates by Saccharomyces cerevisiae were studied. The optimized glucose concentration produced from hydrolysis was used in th...

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Main Authors: Kamaruddin, Mohd Hider, Mel, Maizirwan
Format: Conference or Workshop Item
Language:English
Published: 2010
Subjects:
Online Access:http://irep.iium.edu.my/4993/
http://irep.iium.edu.my/4993/
http://irep.iium.edu.my/4993/1/ICARET_2010_Page_83-87.pdf
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recordtype eprints
spelling iium-49932011-11-25T05:07:04Z http://irep.iium.edu.my/4993/ Optimizing Bioethanol Production from Sago Starch in Bioreactor for Renewable Energy Kamaruddin, Mohd Hider Mel, Maizirwan TP Chemical technology TP248.13 Biotechnology TP500 Fermentation industries. Beverages. Alcohol The two-step enzymatic hydrolysis of sago starch was performed by commercially available α-amylase and β- glucoamylase and further ethanol fermentation of the obtained hydrolyzates by Saccharomyces cerevisiae were studied. The optimized glucose concentration produced from hydrolysis was used in the fermentation process. The fermentation was carried out in a two liters stirred tank bioreactor (Sartorius) by using process conditions that are aeration and agitation. Agitation speed was varied from 50 to 100 rpm and aeration was varied from 0.05 to 0.1 vvm. Nine runs were conducted from the experimental design of central composite design (Minitab software v 14.01). Each running was completed within 72 hours. The analysis of the ethanol sample concentration was done for every 12 hours by using Potassium Di-Chromate test. The highest concentration of ethanol obtained is 11.81 % for run 7 after 60 hours fermentation time. From ANOVA analysis has shown that the aeration is the most significant factor with given “Prob>F” value of 0.032 (“Prob>F”< 0.05). Critical values were obtained at 0.08 vvm and 75 rpm for aeration and agitation, respectively. The findings of this project can be applied in production of bioethanol and biofuel research in large scale. 2010-07 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/4993/1/ICARET_2010_Page_83-87.pdf Kamaruddin, Mohd Hider and Mel, Maizirwan (2010) Optimizing Bioethanol Production from Sago Starch in Bioreactor for Renewable Energy. In: International Conference on Advances in Renewable Energy Technologies, 6-7 July 2010, Cyberjaya, Malaysia. http://www.uniten.edu.my/newhome/content_list.asp?contentid=3692
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic TP Chemical technology
TP248.13 Biotechnology
TP500 Fermentation industries. Beverages. Alcohol
spellingShingle TP Chemical technology
TP248.13 Biotechnology
TP500 Fermentation industries. Beverages. Alcohol
Kamaruddin, Mohd Hider
Mel, Maizirwan
Optimizing Bioethanol Production from Sago Starch in Bioreactor for Renewable Energy
description The two-step enzymatic hydrolysis of sago starch was performed by commercially available α-amylase and β- glucoamylase and further ethanol fermentation of the obtained hydrolyzates by Saccharomyces cerevisiae were studied. The optimized glucose concentration produced from hydrolysis was used in the fermentation process. The fermentation was carried out in a two liters stirred tank bioreactor (Sartorius) by using process conditions that are aeration and agitation. Agitation speed was varied from 50 to 100 rpm and aeration was varied from 0.05 to 0.1 vvm. Nine runs were conducted from the experimental design of central composite design (Minitab software v 14.01). Each running was completed within 72 hours. The analysis of the ethanol sample concentration was done for every 12 hours by using Potassium Di-Chromate test. The highest concentration of ethanol obtained is 11.81 % for run 7 after 60 hours fermentation time. From ANOVA analysis has shown that the aeration is the most significant factor with given “Prob>F” value of 0.032 (“Prob>F”< 0.05). Critical values were obtained at 0.08 vvm and 75 rpm for aeration and agitation, respectively. The findings of this project can be applied in production of bioethanol and biofuel research in large scale.
format Conference or Workshop Item
author Kamaruddin, Mohd Hider
Mel, Maizirwan
author_facet Kamaruddin, Mohd Hider
Mel, Maizirwan
author_sort Kamaruddin, Mohd Hider
title Optimizing Bioethanol Production from Sago Starch in Bioreactor for Renewable Energy
title_short Optimizing Bioethanol Production from Sago Starch in Bioreactor for Renewable Energy
title_full Optimizing Bioethanol Production from Sago Starch in Bioreactor for Renewable Energy
title_fullStr Optimizing Bioethanol Production from Sago Starch in Bioreactor for Renewable Energy
title_full_unstemmed Optimizing Bioethanol Production from Sago Starch in Bioreactor for Renewable Energy
title_sort optimizing bioethanol production from sago starch in bioreactor for renewable energy
publishDate 2010
url http://irep.iium.edu.my/4993/
http://irep.iium.edu.my/4993/
http://irep.iium.edu.my/4993/1/ICARET_2010_Page_83-87.pdf
first_indexed 2023-09-18T20:13:26Z
last_indexed 2023-09-18T20:13:26Z
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