Cocoa pod husk: a new source of CLEA-lipase for preparation of low-cost biodiesel: An optimized process

Cocoa pod husk (CPH) is a by-product of cocoa production obtained after removing the beans from the fruit. The analysis of CPH has shown that it contains high amounts of protein. This study is aimed to utilize this protein source in hydrolase enzyme production. In this study, seven hydrolase enzym...

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Main Authors:	Khanahmadi, Soofia, Yusof, Faridah, Ong, Hwai Chyuan, Amid, Azura, Shah, Harmen
Format:	Article
Language:	English English
Published:	Elsevier 2016
Subjects:	TP Chemical technology TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/51188/ http://irep.iium.edu.my/51188/ http://irep.iium.edu.my/51188/ http://irep.iium.edu.my/51188/1/51188_Cocoa%20pod%20husk%20new%20source%20of%20CLEA-lipase.pdf http://irep.iium.edu.my/51188/2/51188_Cocoa%20pod%20husk%20new%20source%20of%20CLEA-lipase_SCOPUS.pdf

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spelling	iium-511882017-04-05T07:58:30Z http://irep.iium.edu.my/51188/ Cocoa pod husk: a new source of CLEA-lipase for preparation of low-cost biodiesel: An optimized process Khanahmadi, Soofia Yusof, Faridah Ong, Hwai Chyuan Amid, Azura Shah, Harmen TP Chemical technology TP248.13 Biotechnology Cocoa pod husk (CPH) is a by-product of cocoa production obtained after removing the beans from the fruit. The analysis of CPH has shown that it contains high amounts of protein. This study is aimed to utilize this protein source in hydrolase enzyme production. In this study, seven hydrolase enzymes (amylase, fructosyltransferase, mannanase,glucosidase, glucanase, lipase and protease) were screened from CPH for the first time for feasible industrial production. Among these hydrolases, lipase was chosen for the next steps of experiments as it has a lot of applications in different industries.The extraction of high active lipase from CPH has been done under optimum conditions.The condition that was optimum for the three major factors was achieved using Face centered central composite design (FCCCD) with response surface methodology (RSM) to obtain the highest enzyme activity of crude lipase from CPH. The optimum condition of extraction is used for preparation of cross-linked enzyme aggregate (CLEA). For the production of immobilized biocatalyst, the technique of CLEA is considered as an effective technique for its industrially attractive advantages. Referring to the results of OFAT, CLEA-lipase was prepared in the best condition at the presence of 30 mM ammonium sulphate, 70 mM glutaraldehyde with 0.23 mM Bovine serum albumin as an additive. Immobilization effectively improved the stability of lipase against various organic solvents Elsevier 2016 Article PeerReviewed application/pdf en http://irep.iium.edu.my/51188/1/51188_Cocoa%20pod%20husk%20new%20source%20of%20CLEA-lipase.pdf application/pdf en http://irep.iium.edu.my/51188/2/51188_Cocoa%20pod%20husk%20new%20source%20of%20CLEA-lipase_SCOPUS.pdf Khanahmadi, Soofia and Yusof, Faridah and Ong, Hwai Chyuan and Amid, Azura and Shah, Harmen (2016) Cocoa pod husk: a new source of CLEA-lipase for preparation of low-cost biodiesel: An optimized process. Journal of Biotechnology, 231. pp. 95-105. ISSN 0168-1656 E-ISSN 1873-4863 http://www.sciencedirect.com/science/article/pii/S0168165616302711 10.1016/j.jbiotec, 2016.05.015
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	TP Chemical technology TP248.13 Biotechnology
spellingShingle	TP Chemical technology TP248.13 Biotechnology Khanahmadi, Soofia Yusof, Faridah Ong, Hwai Chyuan Amid, Azura Shah, Harmen Cocoa pod husk: a new source of CLEA-lipase for preparation of low-cost biodiesel: An optimized process
description	Cocoa pod husk (CPH) is a by-product of cocoa production obtained after removing the beans from the fruit. The analysis of CPH has shown that it contains high amounts of protein. This study is aimed to utilize this protein source in hydrolase enzyme production. In this study, seven hydrolase enzymes (amylase, fructosyltransferase, mannanase,glucosidase, glucanase, lipase and protease) were screened from CPH for the first time for feasible industrial production. Among these hydrolases, lipase was chosen for the next steps of experiments as it has a lot of applications in different industries.The extraction of high active lipase from CPH has been done under optimum conditions.The condition that was optimum for the three major factors was achieved using Face centered central composite design (FCCCD) with response surface methodology (RSM) to obtain the highest enzyme activity of crude lipase from CPH. The optimum condition of extraction is used for preparation of cross-linked enzyme aggregate (CLEA). For the production of immobilized biocatalyst, the technique of CLEA is considered as an effective technique for its industrially attractive advantages. Referring to the results of OFAT, CLEA-lipase was prepared in the best condition at the presence of 30 mM ammonium sulphate, 70 mM glutaraldehyde with 0.23 mM Bovine serum albumin as an additive. Immobilization effectively improved the stability of lipase against various organic solvents
format	Article
author	Khanahmadi, Soofia Yusof, Faridah Ong, Hwai Chyuan Amid, Azura Shah, Harmen
author_facet	Khanahmadi, Soofia Yusof, Faridah Ong, Hwai Chyuan Amid, Azura Shah, Harmen
author_sort	Khanahmadi, Soofia
title	Cocoa pod husk: a new source of CLEA-lipase for preparation of low-cost biodiesel: An optimized process
title_short	Cocoa pod husk: a new source of CLEA-lipase for preparation of low-cost biodiesel: An optimized process
title_full	Cocoa pod husk: a new source of CLEA-lipase for preparation of low-cost biodiesel: An optimized process
title_fullStr	Cocoa pod husk: a new source of CLEA-lipase for preparation of low-cost biodiesel: An optimized process
title_full_unstemmed	Cocoa pod husk: a new source of CLEA-lipase for preparation of low-cost biodiesel: An optimized process
title_sort	cocoa pod husk: a new source of clea-lipase for preparation of low-cost biodiesel: an optimized process
publisher	Elsevier
publishDate	2016
url	http://irep.iium.edu.my/51188/ http://irep.iium.edu.my/51188/ http://irep.iium.edu.my/51188/ http://irep.iium.edu.my/51188/1/51188_Cocoa%20pod%20husk%20new%20source%20of%20CLEA-lipase.pdf http://irep.iium.edu.my/51188/2/51188_Cocoa%20pod%20husk%20new%20source%20of%20CLEA-lipase_SCOPUS.pdf
first_indexed	2023-09-18T21:12:27Z
last_indexed	2023-09-18T21:12:27Z
_version_	1777411342699855872

Cocoa pod husk: a new source of CLEA-lipase for preparation of low-cost biodiesel: An optimized process

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