Targeting double genes in multiplex PCR for discriminating bovine,buffalo and porcine materials in food chain

Beef, buffalo and pork are the major meat of economic, religious and health concern. Current methods to authenticate these materials in food chain are based on mainly single gene targets which are susceptible to break down by food processing treatments. We, for the first time, described here a doubl...

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Main Authors:	Hossain, Mohammad Amzad Motalib, Ali, Md Eaqub, Abd Hamid, Sharifah EeBee Ee, ..,, Asing, Mustafa, Shuhaimi, Mohd Desa, Mohd Nasir, Sarker, Md. Zaidul Islam
Format:	Article
Language:	English English English
Published:	Elsevier Ltd 2017
Subjects:	R Medicine (General)
Online Access:	http://irep.iium.edu.my/53774/ http://irep.iium.edu.my/53774/ http://irep.iium.edu.my/53774/ http://irep.iium.edu.my/53774/2/53774_Targeting%20double_SCOPUS.pdf http://irep.iium.edu.my/53774/13/53774.pdf http://irep.iium.edu.my/53774/19/53774_Targeting%20double%20genes%20_WOS.pdf

id	iium-53774
recordtype	eprints
spelling	iium-537742017-07-19T00:40:10Z http://irep.iium.edu.my/53774/ Targeting double genes in multiplex PCR for discriminating bovine,buffalo and porcine materials in food chain Hossain, Mohammad Amzad Motalib Ali, Md Eaqub Abd Hamid, Sharifah EeBee Ee ..,, Asing Mustafa, Shuhaimi Mohd Desa, Mohd Nasir Sarker, Md. Zaidul Islam R Medicine (General) Beef, buffalo and pork are the major meat of economic, religious and health concern. Current methods to authenticate these materials in food chain are based on mainly single gene targets which are susceptible to break down by food processing treatments. We, for the first time, described here a double gene targeting short-amplicon length multiplex polymerase chain reaction assay for discriminating bovine, buffalo and porcine materials in a single assay platform. The advantage of the assay is evidenced in terms of fidelity, cost and time since it is highly unlikely that two different targets would be missing even in a decomposed specimen. Detection of multiple targets in a single assay definitely saves analytical cost and time. Mitochondrial cytochrome b (cytb) and ND5 genes were targeted and six different targets (length: 90–146 bp), two for each of cow (120 and 106bp), buffalo (90 and 138bp) and pig (73 and 146bp), were amplified from raw, boiled, autoclaved and microwaved cooked meat under pure and mixed matrices. The detection limit was 0.02 ng DNA under pure states and 0.1% meat in binary mixtures and meatball products. Screening of Malaysian meatball products revealed all beef products were buffalo positive in which 35% were totally replaced. In contrast, all pork products were found uncontaminated from beef and buffalo. Elsevier Ltd 2017-03-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/53774/2/53774_Targeting%20double_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/53774/13/53774.pdf application/pdf en http://irep.iium.edu.my/53774/19/53774_Targeting%20double%20genes%20_WOS.pdf Hossain, Mohammad Amzad Motalib and Ali, Md Eaqub and Abd Hamid, Sharifah EeBee Ee and ..,, Asing and Mustafa, Shuhaimi and Mohd Desa, Mohd Nasir and Sarker, Md. Zaidul Islam (2017) Targeting double genes in multiplex PCR for discriminating bovine,buffalo and porcine materials in food chain. Food Control, 73. pp. 175-184. ISSN 0956-7135 http://www.sciencedirect.com/science/article/pii/S0956713516304261 10.1016/j.foodcont.2016.08.008
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Hossain, Mohammad Amzad Motalib Ali, Md Eaqub Abd Hamid, Sharifah EeBee Ee ..,, Asing Mustafa, Shuhaimi Mohd Desa, Mohd Nasir Sarker, Md. Zaidul Islam Targeting double genes in multiplex PCR for discriminating bovine,buffalo and porcine materials in food chain
description	Beef, buffalo and pork are the major meat of economic, religious and health concern. Current methods to authenticate these materials in food chain are based on mainly single gene targets which are susceptible to break down by food processing treatments. We, for the first time, described here a double gene targeting short-amplicon length multiplex polymerase chain reaction assay for discriminating bovine, buffalo and porcine materials in a single assay platform. The advantage of the assay is evidenced in terms of fidelity, cost and time since it is highly unlikely that two different targets would be missing even in a decomposed specimen. Detection of multiple targets in a single assay definitely saves analytical cost and time. Mitochondrial cytochrome b (cytb) and ND5 genes were targeted and six different targets (length: 90–146 bp), two for each of cow (120 and 106bp), buffalo (90 and 138bp) and pig (73 and 146bp), were amplified from raw, boiled, autoclaved and microwaved cooked meat under pure and mixed matrices. The detection limit was 0.02 ng DNA under pure states and 0.1% meat in binary mixtures and meatball products. Screening of Malaysian meatball products revealed all beef products were buffalo positive in which 35% were totally replaced. In contrast, all pork products were found uncontaminated from beef and buffalo.
format	Article
author	Hossain, Mohammad Amzad Motalib Ali, Md Eaqub Abd Hamid, Sharifah EeBee Ee ..,, Asing Mustafa, Shuhaimi Mohd Desa, Mohd Nasir Sarker, Md. Zaidul Islam
author_facet	Hossain, Mohammad Amzad Motalib Ali, Md Eaqub Abd Hamid, Sharifah EeBee Ee ..,, Asing Mustafa, Shuhaimi Mohd Desa, Mohd Nasir Sarker, Md. Zaidul Islam
author_sort	Hossain, Mohammad Amzad Motalib
title	Targeting double genes in multiplex PCR for discriminating bovine,buffalo and porcine materials in food chain
title_short	Targeting double genes in multiplex PCR for discriminating bovine,buffalo and porcine materials in food chain
title_full	Targeting double genes in multiplex PCR for discriminating bovine,buffalo and porcine materials in food chain
title_fullStr	Targeting double genes in multiplex PCR for discriminating bovine,buffalo and porcine materials in food chain
title_full_unstemmed	Targeting double genes in multiplex PCR for discriminating bovine,buffalo and porcine materials in food chain
title_sort	targeting double genes in multiplex pcr for discriminating bovine,buffalo and porcine materials in food chain
publisher	Elsevier Ltd
publishDate	2017
url	http://irep.iium.edu.my/53774/ http://irep.iium.edu.my/53774/ http://irep.iium.edu.my/53774/ http://irep.iium.edu.my/53774/2/53774_Targeting%20double_SCOPUS.pdf http://irep.iium.edu.my/53774/13/53774.pdf http://irep.iium.edu.my/53774/19/53774_Targeting%20double%20genes%20_WOS.pdf
first_indexed	2023-09-18T21:16:04Z
last_indexed	2023-09-18T21:16:04Z
_version_	1777411570105581568

Targeting double genes in multiplex PCR for discriminating bovine,buffalo and porcine materials in food chain

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