Effects of triterpenoids from Luvunga scandens on cytotoxic, cell cycle arrest and gene expressions in MCF-7 cells

Plant-derived triterpenoids commonly possesses biological properties such as anti-inflammatory, antimicrobial, anti-viral and anti-cancer. Luvunga scandens is one of the plant that produced triterpenoids. The aims of the study was to analyze cell cycle profile and to determine the expression of p53...

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Main Authors:	Bakhtiar, M. Taher, Al-Zikri, Putri Nur Hidayah, Darnis, Deny Susanti, Arief Ichwan, Solachuddin Jauhari, Rezali, Mohd Fazlin
Format:	Article
Language:	English English
Published:	The Korean Society of Pharmacognosy 2016
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/54878/ http://irep.iium.edu.my/54878/ http://irep.iium.edu.my/54878/ http://irep.iium.edu.my/54878/1/Nat%20Prod%20Sci.pdf http://irep.iium.edu.my/54878/7/54878_Effects%20of%20triterpenoids_Scopus.pdf

id	iium-54878
recordtype	eprints
spelling	iium-548782017-04-13T07:17:23Z http://irep.iium.edu.my/54878/ Effects of triterpenoids from Luvunga scandens on cytotoxic, cell cycle arrest and gene expressions in MCF-7 cells Bakhtiar, M. Taher Al-Zikri, Putri Nur Hidayah Darnis, Deny Susanti Arief Ichwan, Solachuddin Jauhari Rezali, Mohd Fazlin RS Pharmacy and materia medica Plant-derived triterpenoids commonly possesses biological properties such as anti-inflammatory, antimicrobial, anti-viral and anti-cancer. Luvunga scandens is one of the plant that produced triterpenoids. The aims of the study was to analyze cell cycle profile and to determine the expression of p53 unregulated modulator of apoptosis (PUMA), caspase-8 and caspase-9 genes at mRNA level in MCF-7 cell line treated with two triterpenoids, flindissol (1) and 3-oxotirucalla-7,24-dien-21-oic-acid (2) isolated from L. scandens. The compounds were tested for cell cycle analysis using flow cytometer and mRNA expression level using quantitative RT-PCR. The number of MCF-7 cells population which distributed in Sub G1 phase after treated with compound 1 and 2 were 7.7 and 9.3% respectively. The evaluation of the expression of genes showed that both compounds exhibited high level of expression of PUMA, caspase-8 and caspase-9 as normalized to β-actin via activation of those genes. In summary, the isolated compounds of L. scandens plant showed promising anticancer properties in MCF- 7 cell lines. The Korean Society of Pharmacognosy 2016 Article PeerReviewed application/pdf en http://irep.iium.edu.my/54878/1/Nat%20Prod%20Sci.pdf application/pdf en http://irep.iium.edu.my/54878/7/54878_Effects%20of%20triterpenoids_Scopus.pdf Bakhtiar, M. Taher and Al-Zikri, Putri Nur Hidayah and Darnis, Deny Susanti and Arief Ichwan, Solachuddin Jauhari and Rezali, Mohd Fazlin (2016) Effects of triterpenoids from Luvunga scandens on cytotoxic, cell cycle arrest and gene expressions in MCF-7 cells. Natural Product Science, 22 (4). pp. 293-298. ISSN 1226-3907 E-ISSN 2288-9027 https://synapse.koreamed.org/DOIx.php?id=10.20307/nps.2016.22.4.293 doi.org/10.2307/nps.2016.22.4.293
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Bakhtiar, M. Taher Al-Zikri, Putri Nur Hidayah Darnis, Deny Susanti Arief Ichwan, Solachuddin Jauhari Rezali, Mohd Fazlin Effects of triterpenoids from Luvunga scandens on cytotoxic, cell cycle arrest and gene expressions in MCF-7 cells
description	Plant-derived triterpenoids commonly possesses biological properties such as anti-inflammatory, antimicrobial, anti-viral and anti-cancer. Luvunga scandens is one of the plant that produced triterpenoids. The aims of the study was to analyze cell cycle profile and to determine the expression of p53 unregulated modulator of apoptosis (PUMA), caspase-8 and caspase-9 genes at mRNA level in MCF-7 cell line treated with two triterpenoids, flindissol (1) and 3-oxotirucalla-7,24-dien-21-oic-acid (2) isolated from L. scandens. The compounds were tested for cell cycle analysis using flow cytometer and mRNA expression level using quantitative RT-PCR. The number of MCF-7 cells population which distributed in Sub G1 phase after treated with compound 1 and 2 were 7.7 and 9.3% respectively. The evaluation of the expression of genes showed that both compounds exhibited high level of expression of PUMA, caspase-8 and caspase-9 as normalized to β-actin via activation of those genes. In summary, the isolated compounds of L. scandens plant showed promising anticancer properties in MCF- 7 cell lines.
format	Article
author	Bakhtiar, M. Taher Al-Zikri, Putri Nur Hidayah Darnis, Deny Susanti Arief Ichwan, Solachuddin Jauhari Rezali, Mohd Fazlin
author_facet	Bakhtiar, M. Taher Al-Zikri, Putri Nur Hidayah Darnis, Deny Susanti Arief Ichwan, Solachuddin Jauhari Rezali, Mohd Fazlin
author_sort	Bakhtiar, M. Taher
title	Effects of triterpenoids from Luvunga scandens on cytotoxic, cell cycle arrest and gene expressions in MCF-7 cells
title_short	Effects of triterpenoids from Luvunga scandens on cytotoxic, cell cycle arrest and gene expressions in MCF-7 cells
title_full	Effects of triterpenoids from Luvunga scandens on cytotoxic, cell cycle arrest and gene expressions in MCF-7 cells
title_fullStr	Effects of triterpenoids from Luvunga scandens on cytotoxic, cell cycle arrest and gene expressions in MCF-7 cells
title_full_unstemmed	Effects of triterpenoids from Luvunga scandens on cytotoxic, cell cycle arrest and gene expressions in MCF-7 cells
title_sort	effects of triterpenoids from luvunga scandens on cytotoxic, cell cycle arrest and gene expressions in mcf-7 cells
publisher	The Korean Society of Pharmacognosy
publishDate	2016
url	http://irep.iium.edu.my/54878/ http://irep.iium.edu.my/54878/ http://irep.iium.edu.my/54878/ http://irep.iium.edu.my/54878/1/Nat%20Prod%20Sci.pdf http://irep.iium.edu.my/54878/7/54878_Effects%20of%20triterpenoids_Scopus.pdf
first_indexed	2023-09-18T21:17:35Z
last_indexed	2023-09-18T21:17:35Z
_version_	1777411665875173376

Effects of triterpenoids from Luvunga scandens on cytotoxic, cell cycle arrest and gene expressions in MCF-7 cells

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