Enzyme immunoassay for the detection of porcine gelatine in edible bird’s nests
Porcine gelatine is a common adulterant found in edible bird’s nests (EBNs) used to increase the net weight prior to sale. This study aimed to develop indirect enzyme-linked immunosorbent assays (ELISAs) for porcine gelatine adulteration using anti-peptide polyclonal antibodies. Three indirect ELI...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Taylor & Francis Group
2015
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/55414/ http://irep.iium.edu.my/55414/ http://irep.iium.edu.my/55414/ http://irep.iium.edu.my/55414/7/55414-edited.pdf http://irep.iium.edu.my/55414/8/55414-Enzyme%20immunoassay%20for%20the%20detection%20of%20porcine%20gelatine%20in%20edible%20bird%E2%80%99s%20nests_SCOPUS.pdf |
| Summary: | Porcine gelatine is a common adulterant found in edible bird’s nests (EBNs) used to increase the net weight prior to sale.
This study aimed to develop indirect enzyme-linked immunosorbent assays (ELISAs) for porcine gelatine adulteration using
anti-peptide polyclonal antibodies. Three indirect ELISAs were developed (PAB1, 2 and 3), which had limits of detection
(LODs) of 0.12, 0.10 and 0.11 μg g–1, respectively. When applied to standard solutions of porcine gelatine, the inter- and
intra-assays showed coefficients of variation (CVs) less than 20% and were able to detect at least 0.5 ng μg–1 (0.05%)
porcine gelatine in spiked samples. The proposed ELISA offers attractions for quality control in the EBN industry. |
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