Enzyme immunoassay for the detection of porcine gelatine in edible bird’s nests

Porcine gelatine is a common adulterant found in edible bird’s nests (EBNs) used to increase the net weight prior to sale. This study aimed to develop indirect enzyme-linked immunosorbent assays (ELISAs) for porcine gelatine adulteration using anti-peptide polyclonal antibodies. Three indirect ELI...

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Main Authors:	Tukiran, Nur Azira, Ismail, Amin, Mustafa, Shuhaimi, Hamid, Muhajir
Format:	Article
Language:	English English
Published:	Taylor & Francis Group 2015
Subjects:	TP Chemical technology
Online Access:	http://irep.iium.edu.my/55414/ http://irep.iium.edu.my/55414/ http://irep.iium.edu.my/55414/ http://irep.iium.edu.my/55414/7/55414-edited.pdf http://irep.iium.edu.my/55414/8/55414-Enzyme%20immunoassay%20for%20the%20detection%20of%20porcine%20gelatine%20in%20edible%20bird%E2%80%99s%20nests_SCOPUS.pdf

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recordtype	eprints
spelling	iium-554142017-07-18T00:40:02Z http://irep.iium.edu.my/55414/ Enzyme immunoassay for the detection of porcine gelatine in edible bird’s nests Tukiran, Nur Azira Ismail, Amin Mustafa, Shuhaimi Hamid, Muhajir TP Chemical technology Porcine gelatine is a common adulterant found in edible bird’s nests (EBNs) used to increase the net weight prior to sale. This study aimed to develop indirect enzyme-linked immunosorbent assays (ELISAs) for porcine gelatine adulteration using anti-peptide polyclonal antibodies. Three indirect ELISAs were developed (PAB1, 2 and 3), which had limits of detection (LODs) of 0.12, 0.10 and 0.11 μg g–1, respectively. When applied to standard solutions of porcine gelatine, the inter- and intra-assays showed coefficients of variation (CVs) less than 20% and were able to detect at least 0.5 ng μg–1 (0.05%) porcine gelatine in spiked samples. The proposed ELISA offers attractions for quality control in the EBN industry. Taylor & Francis Group 2015 Article PeerReviewed application/pdf en http://irep.iium.edu.my/55414/7/55414-edited.pdf application/pdf en http://irep.iium.edu.my/55414/8/55414-Enzyme%20immunoassay%20for%20the%20detection%20of%20porcine%20gelatine%20in%20edible%20bird%E2%80%99s%20nests_SCOPUS.pdf Tukiran, Nur Azira and Ismail, Amin and Mustafa, Shuhaimi and Hamid, Muhajir (2015) Enzyme immunoassay for the detection of porcine gelatine in edible bird’s nests. Food Additives and Contaminants: Part A, 32 (7). pp. 1023-1028. ISSN 1944-0049 E-ISSN 1944-0057 http://www.tandfonline.com/doi/abs/10.1080/19440049.2015.1039605 10.1080/19440049.2015.1039605
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Tukiran, Nur Azira Ismail, Amin Mustafa, Shuhaimi Hamid, Muhajir Enzyme immunoassay for the detection of porcine gelatine in edible bird’s nests
description	Porcine gelatine is a common adulterant found in edible bird’s nests (EBNs) used to increase the net weight prior to sale. This study aimed to develop indirect enzyme-linked immunosorbent assays (ELISAs) for porcine gelatine adulteration using anti-peptide polyclonal antibodies. Three indirect ELISAs were developed (PAB1, 2 and 3), which had limits of detection (LODs) of 0.12, 0.10 and 0.11 μg g–1, respectively. When applied to standard solutions of porcine gelatine, the inter- and intra-assays showed coefficients of variation (CVs) less than 20% and were able to detect at least 0.5 ng μg–1 (0.05%) porcine gelatine in spiked samples. The proposed ELISA offers attractions for quality control in the EBN industry.
format	Article
author	Tukiran, Nur Azira Ismail, Amin Mustafa, Shuhaimi Hamid, Muhajir
author_facet	Tukiran, Nur Azira Ismail, Amin Mustafa, Shuhaimi Hamid, Muhajir
author_sort	Tukiran, Nur Azira
title	Enzyme immunoassay for the detection of porcine gelatine in edible bird’s nests
title_short	Enzyme immunoassay for the detection of porcine gelatine in edible bird’s nests
title_full	Enzyme immunoassay for the detection of porcine gelatine in edible bird’s nests
title_fullStr	Enzyme immunoassay for the detection of porcine gelatine in edible bird’s nests
title_full_unstemmed	Enzyme immunoassay for the detection of porcine gelatine in edible bird’s nests
title_sort	enzyme immunoassay for the detection of porcine gelatine in edible bird’s nests
publisher	Taylor & Francis Group
publishDate	2015
url	http://irep.iium.edu.my/55414/ http://irep.iium.edu.my/55414/ http://irep.iium.edu.my/55414/ http://irep.iium.edu.my/55414/7/55414-edited.pdf http://irep.iium.edu.my/55414/8/55414-Enzyme%20immunoassay%20for%20the%20detection%20of%20porcine%20gelatine%20in%20edible%20bird%E2%80%99s%20nests_SCOPUS.pdf
first_indexed	2023-09-18T21:18:19Z
last_indexed	2023-09-18T21:18:19Z
_version_	1777411712527368192

Enzyme immunoassay for the detection of porcine gelatine in edible bird’s nests

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