Immunofluorescence evidence of cryptosporidium in water

Cryptosporidium causes cryptosporidiosis which can cause serious health effects especially to immunocompromised people. While this parasite can spread in several ways, transmission through ingestion of infected water is the most common way. Many studies have been conducted in several countries perta...

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Main Authors:	Zainutdin, Fatin Khairunnisa, Md Isa, Muhammad Lokman, Mat Yusof, Afzan
Format:	Conference or Workshop Item
Language:	English
Published:	2016
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/56834/ http://irep.iium.edu.my/56834/1/Conference%20Immunofluorescence.pdf

id	iium-56834
recordtype	eprints
spelling	iium-568342018-05-18T07:02:53Z http://irep.iium.edu.my/56834/ Immunofluorescence evidence of cryptosporidium in water Zainutdin, Fatin Khairunnisa Md Isa, Muhammad Lokman Mat Yusof, Afzan Q Science (General) Cryptosporidium causes cryptosporidiosis which can cause serious health effects especially to immunocompromised people. While this parasite can spread in several ways, transmission through ingestion of infected water is the most common way. Many studies have been conducted in several countries pertaining to the occurrence of Cryptosporidium in water sources including Malaysia. However, the information and data collected for the occurrence of Cryptosporidium in river water, especially in state of Pahang is still insufficient. Hence, the study aimed to determine the occurrence of Cryptosporidium oocysts in two main rivers in Kuantan. Water samples were collected and processed immediately after each sample collection. Cryptosporidium oocysts were isolated by using immunomagnetic separation technique and were stained using FITC-belled anti-crypto sp monoclonal antibody stain and observed under immunofluorescence microscope. The result showed that all water samples were positive with Cryptosporidium oocysts. The oocysts were stained in fluorescence green and appear to be in spherical shape ranging from 4-6µm in size under immunofluorescence microscope. Shedding of infected faeces from humans or animals into the river is believed to be a major contributor to the occurrence of Cryptosporidium oocysts in water. The findings can provide useful information regarding the occurrence of Cryptosporidium oocysts in water. 2016-12-07 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/56834/1/Conference%20Immunofluorescence.pdf Zainutdin, Fatin Khairunnisa and Md Isa, Muhammad Lokman and Mat Yusof, Afzan (2016) Immunofluorescence evidence of cryptosporidium in water. In: 25th Scientific Conference of the Microscopy Society Malaysia (25th SCMSM 2016), 7th-8th Dec. 2017, Hotel Bangi Putrajaya, Selangor. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Zainutdin, Fatin Khairunnisa Md Isa, Muhammad Lokman Mat Yusof, Afzan Immunofluorescence evidence of cryptosporidium in water
description	Cryptosporidium causes cryptosporidiosis which can cause serious health effects especially to immunocompromised people. While this parasite can spread in several ways, transmission through ingestion of infected water is the most common way. Many studies have been conducted in several countries pertaining to the occurrence of Cryptosporidium in water sources including Malaysia. However, the information and data collected for the occurrence of Cryptosporidium in river water, especially in state of Pahang is still insufficient. Hence, the study aimed to determine the occurrence of Cryptosporidium oocysts in two main rivers in Kuantan. Water samples were collected and processed immediately after each sample collection. Cryptosporidium oocysts were isolated by using immunomagnetic separation technique and were stained using FITC-belled anti-crypto sp monoclonal antibody stain and observed under immunofluorescence microscope. The result showed that all water samples were positive with Cryptosporidium oocysts. The oocysts were stained in fluorescence green and appear to be in spherical shape ranging from 4-6µm in size under immunofluorescence microscope. Shedding of infected faeces from humans or animals into the river is believed to be a major contributor to the occurrence of Cryptosporidium oocysts in water. The findings can provide useful information regarding the occurrence of Cryptosporidium oocysts in water.
format	Conference or Workshop Item
author	Zainutdin, Fatin Khairunnisa Md Isa, Muhammad Lokman Mat Yusof, Afzan
author_facet	Zainutdin, Fatin Khairunnisa Md Isa, Muhammad Lokman Mat Yusof, Afzan
author_sort	Zainutdin, Fatin Khairunnisa
title	Immunofluorescence evidence of cryptosporidium in water
title_short	Immunofluorescence evidence of cryptosporidium in water
title_full	Immunofluorescence evidence of cryptosporidium in water
title_fullStr	Immunofluorescence evidence of cryptosporidium in water
title_full_unstemmed	Immunofluorescence evidence of cryptosporidium in water
title_sort	immunofluorescence evidence of cryptosporidium in water
publishDate	2016
url	http://irep.iium.edu.my/56834/ http://irep.iium.edu.my/56834/1/Conference%20Immunofluorescence.pdf
first_indexed	2023-09-18T21:20:14Z
last_indexed	2023-09-18T21:20:14Z
_version_	1777411832794841088

Immunofluorescence evidence of cryptosporidium in water

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