Indirect competitive enzyme-linked immunosorbent assay (ELISA) for the determination of mammalian gelatin in pharmaceutical capsules
Gelatin is derived from partial hydrolysis of type I collagen (connective tissues such as skin, bone, tendon, and ligament), which is commonly obtained from mammalian sources (porcine and bovine). However, the use of gelatin derived from mammalian sources in pharmaceutical products have become a con...
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2018
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| Online Access: | http://irep.iium.edu.my/68682/ http://irep.iium.edu.my/68682/ http://irep.iium.edu.my/68682/ http://irep.iium.edu.my/68682/1/Paper%20IHAC%202018.pdf |
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iium-686822019-07-15T03:13:41Z http://irep.iium.edu.my/68682/ Indirect competitive enzyme-linked immunosorbent assay (ELISA) for the determination of mammalian gelatin in pharmaceutical capsules Tukiran, Nur Azira Ismail, Amin Mustafa, Shuhaimi Hamid, Muhajir Q Science (General) Q128 Islam and Science QD Chemistry Gelatin is derived from partial hydrolysis of type I collagen (connective tissues such as skin, bone, tendon, and ligament), which is commonly obtained from mammalian sources (porcine and bovine). However, the use of gelatin derived from mammalian sources in pharmaceutical products have become a controversial issue regarding religious and health concern. Thus, the aim of this study was to develop indirect competitive enzyme-linked immunosorbent assays (ELISAs) based on antipeptide polyclonal antibodies (pAbs) for the determination of mammalian gelatin in pharmaceutical capsules. Polyclonal antibodies against amino acid sequences of collagen α2 (I) chain were used (pAb1 and pAb2). The IC50 value of 0.390 µg/mL and detection limit of 0.082 µg/mL (in buffer) were achieved by ELISA based on pAb2. The intra-assay (<14%) and inter-assay (<13%) coefficients of variation were also acceptable. The ELISA was successfully applied for the determination of mammalian gelatin in pharmaceutical capsule (hard shell capsule, soft shell capsule, and tablet). The result suggests that this ELISA method is useful for determination of mammalian gelatin in selected pharmaceutical capsules. Springer 2018 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/68682/1/Paper%20IHAC%202018.pdf Tukiran, Nur Azira and Ismail, Amin and Mustafa, Shuhaimi and Hamid, Muhajir (2018) Indirect competitive enzyme-linked immunosorbent assay (ELISA) for the determination of mammalian gelatin in pharmaceutical capsules. In: 3rd International Halal Conference (INHAC 2016), 21st-22nd November 2016, Shah Alam, Selangor. https://link.springer.com/chapter/10.1007/978-981-10-7257-4_38 10.1007/978-981-10-7257-4_38 |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
International Islamic University Malaysia |
| building |
IIUM Repository |
| collection |
Online Access |
| language |
English |
| topic |
Q Science (General) Q128 Islam and Science QD Chemistry |
| spellingShingle |
Q Science (General) Q128 Islam and Science QD Chemistry Tukiran, Nur Azira Ismail, Amin Mustafa, Shuhaimi Hamid, Muhajir Indirect competitive enzyme-linked immunosorbent assay (ELISA) for the determination of mammalian gelatin in pharmaceutical capsules |
| description |
Gelatin is derived from partial hydrolysis of type I collagen (connective tissues such as skin, bone, tendon, and ligament), which is commonly obtained from mammalian sources (porcine and bovine). However, the use of gelatin derived from mammalian sources in pharmaceutical products have become a controversial issue regarding religious and health concern. Thus, the aim of this study was to develop indirect competitive enzyme-linked immunosorbent assays (ELISAs) based on antipeptide polyclonal antibodies (pAbs) for the determination of mammalian gelatin in pharmaceutical capsules. Polyclonal antibodies against amino acid sequences of collagen α2 (I) chain were used (pAb1 and pAb2). The IC50 value of 0.390 µg/mL and detection limit of 0.082 µg/mL (in buffer) were achieved by ELISA based on pAb2. The intra-assay (<14%) and inter-assay (<13%) coefficients of variation were also acceptable. The ELISA was successfully applied for the determination of mammalian gelatin in pharmaceutical capsule (hard shell capsule, soft shell capsule, and tablet). The result suggests that this ELISA method is useful for determination of mammalian gelatin in selected pharmaceutical capsules. |
| format |
Conference or Workshop Item |
| author |
Tukiran, Nur Azira Ismail, Amin Mustafa, Shuhaimi Hamid, Muhajir |
| author_facet |
Tukiran, Nur Azira Ismail, Amin Mustafa, Shuhaimi Hamid, Muhajir |
| author_sort |
Tukiran, Nur Azira |
| title |
Indirect competitive enzyme-linked immunosorbent assay (ELISA) for the determination of mammalian gelatin in pharmaceutical capsules |
| title_short |
Indirect competitive enzyme-linked immunosorbent assay (ELISA) for the determination of mammalian gelatin in pharmaceutical capsules |
| title_full |
Indirect competitive enzyme-linked immunosorbent assay (ELISA) for the determination of mammalian gelatin in pharmaceutical capsules |
| title_fullStr |
Indirect competitive enzyme-linked immunosorbent assay (ELISA) for the determination of mammalian gelatin in pharmaceutical capsules |
| title_full_unstemmed |
Indirect competitive enzyme-linked immunosorbent assay (ELISA) for the determination of mammalian gelatin in pharmaceutical capsules |
| title_sort |
indirect competitive enzyme-linked immunosorbent assay (elisa) for the determination of mammalian gelatin in pharmaceutical capsules |
| publisher |
Springer |
| publishDate |
2018 |
| url |
http://irep.iium.edu.my/68682/ http://irep.iium.edu.my/68682/ http://irep.iium.edu.my/68682/ http://irep.iium.edu.my/68682/1/Paper%20IHAC%202018.pdf |
| first_indexed |
2023-09-18T21:37:27Z |
| last_indexed |
2023-09-18T21:37:27Z |
| _version_ |
1777412915774619648 |