Application of two dimensional thin layer chromatography pattern comparison for fingerprinting the active compaounds in the leaves of Vitex Trifolia linn processing anti-tracheospasmolytic activity

Application of two dimensional thin layer chromatography pattern comparison for fingerprinting the active compaounds in the leaves of Vitex Trifolia linn processing anti-tracheospasmolytic activity

We have developed one approach to fingerprint and estimate the active compounds in the leaves of Vitex trifolia Linn possessing anti-tracheospasmolytic assay using two-dimensional TLC pattern comparison. Based on the two-dimensional TLC pattern and the activity of the centrifugal partition chromato...

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Main Authors: Khatib, Alfi, Hoek, Arie C., Jinap, Selamat, Sarker, Md. Zaidul Islam, Jaswir, Irwandi, Verpoorte, Robert
Format: Article
Language:English
Published: Taylor & Francis Group 2010
Subjects:
QD Chemistry
Online Access:http://irep.iium.edu.my/7962/
http://irep.iium.edu.my/7962/
http://irep.iium.edu.my/7962/
http://irep.iium.edu.my/7962/1/irwandi_2010._JLCRT.pdf
id iium-7962
recordtype eprints
spelling iium-79622013-12-16T05:02:06Z http://irep.iium.edu.my/7962/ Application of two dimensional thin layer chromatography pattern comparison for fingerprinting the active compaounds in the leaves of Vitex Trifolia linn processing anti-tracheospasmolytic activity Khatib, Alfi Hoek, Arie C. Jinap, Selamat Sarker, Md. Zaidul Islam Jaswir, Irwandi Verpoorte, Robert QD Chemistry We have developed one approach to fingerprint and estimate the active compounds in the leaves of Vitex trifolia Linn possessing anti-tracheospasmolytic assay using two-dimensional TLC pattern comparison. Based on the two-dimensional TLC pattern and the activity of the centrifugal partition chromatography fractions, we concluded that the semi polar compounds were responsible for anti-tracheospasmolytic activity. The best non-polar=semi polar mobile phases for the two-dimensional TLC using silica gel as the stationary phase were chloroform=methanol (9=1) as the first mobile phase and ethyl cetate=chloroform=methanol (28=28=44) as the second mobile phase. Taylor & Francis Group 2010 Article PeerReviewed application/pdf en http://irep.iium.edu.my/7962/1/irwandi_2010._JLCRT.pdf Khatib, Alfi and Hoek, Arie C. and Jinap, Selamat and Sarker, Md. Zaidul Islam and Jaswir, Irwandi and Verpoorte, Robert (2010) Application of two dimensional thin layer chromatography pattern comparison for fingerprinting the active compaounds in the leaves of Vitex Trifolia linn processing anti-tracheospasmolytic activity. Journal of Liquid Chromatography & Related Technologies, 33. pp. 214-224. ISSN 1082-6076 print/1520-572X online http://www.tandf.co.uk/journals/titles/10826076.asp DOI: 10.1080/10826070903439416
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic QD Chemistry
spellingShingle QD Chemistry
Khatib, Alfi
Hoek, Arie C.
Jinap, Selamat
Sarker, Md. Zaidul Islam
Jaswir, Irwandi
Verpoorte, Robert
Application of two dimensional thin layer chromatography pattern comparison for fingerprinting the active compaounds in the leaves of Vitex Trifolia linn processing anti-tracheospasmolytic activity
description We have developed one approach to fingerprint and estimate the active compounds in the leaves of Vitex trifolia Linn possessing anti-tracheospasmolytic assay using two-dimensional TLC pattern comparison. Based on the two-dimensional TLC pattern and the activity of the centrifugal partition chromatography fractions, we concluded that the semi polar compounds were responsible for anti-tracheospasmolytic activity. The best non-polar=semi polar mobile phases for the two-dimensional TLC using silica gel as the stationary phase were chloroform=methanol (9=1) as the first mobile phase and ethyl cetate=chloroform=methanol (28=28=44) as the second mobile phase.
format Article
author Khatib, Alfi
Hoek, Arie C.
Jinap, Selamat
Sarker, Md. Zaidul Islam
Jaswir, Irwandi
Verpoorte, Robert
author_facet Khatib, Alfi
Hoek, Arie C.
Jinap, Selamat
Sarker, Md. Zaidul Islam
Jaswir, Irwandi
Verpoorte, Robert
author_sort Khatib, Alfi
title Application of two dimensional thin layer chromatography pattern comparison for fingerprinting the active compaounds in the leaves of Vitex Trifolia linn processing anti-tracheospasmolytic activity
title_short Application of two dimensional thin layer chromatography pattern comparison for fingerprinting the active compaounds in the leaves of Vitex Trifolia linn processing anti-tracheospasmolytic activity
title_full Application of two dimensional thin layer chromatography pattern comparison for fingerprinting the active compaounds in the leaves of Vitex Trifolia linn processing anti-tracheospasmolytic activity
title_fullStr Application of two dimensional thin layer chromatography pattern comparison for fingerprinting the active compaounds in the leaves of Vitex Trifolia linn processing anti-tracheospasmolytic activity
title_full_unstemmed Application of two dimensional thin layer chromatography pattern comparison for fingerprinting the active compaounds in the leaves of Vitex Trifolia linn processing anti-tracheospasmolytic activity
title_sort application of two dimensional thin layer chromatography pattern comparison for fingerprinting the active compaounds in the leaves of vitex trifolia linn processing anti-tracheospasmolytic activity
publisher Taylor & Francis Group
publishDate 2010
url http://irep.iium.edu.my/7962/
http://irep.iium.edu.my/7962/
http://irep.iium.edu.my/7962/
http://irep.iium.edu.my/7962/1/irwandi_2010._JLCRT.pdf
first_indexed 2023-09-18T20:17:30Z
last_indexed 2023-09-18T20:17:30Z
_version_ 1777407886156103680

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