The factors affecting the extraction condition for neuroprotective activity of centella asiatica evaluated by metal chelating activity assay

The study was aimed to evaluate the metal chelating activity of CA which will first be assessed for neuroprotective property. Further, response surface methodology will be used to optimize the extraction parameters to yield an optimum metal chelating activity of CA by minimizing the cost of extracti...

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Main Authors:	Amid, Azura, Mohd Salim, Roshan Jahn, Adenan, Mohd Ilham
Format:	Article
Language:	English
Published:	Asian Network for Scientific Information 2010
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/852/ http://irep.iium.edu.my/852/ http://irep.iium.edu.my/852/ http://irep.iium.edu.my/852/1/Centella_asiatica.pdf

id	iium-852
recordtype	eprints
spelling	iium-8522011-12-20T04:00:50Z http://irep.iium.edu.my/852/ The factors affecting the extraction condition for neuroprotective activity of centella asiatica evaluated by metal chelating activity assay Amid, Azura Mohd Salim, Roshan Jahn Adenan, Mohd Ilham TP248.13 Biotechnology The study was aimed to evaluate the metal chelating activity of CA which will first be assessed for neuroprotective property. Further, response surface methodology will be used to optimize the extraction parameters to yield an optimum metal chelating activity of CA by minimizing the cost of extraction. Centella asiatica (CA) is a rich antioxidant candidate was studied for its potential as a neuroprotective agent to fight against oxidative damage caused by Reactive Oxygen Species (ROS) towards neuronal cells which eventually lead to neurodegenerative diseases such as Alzheimer’s and Parkinson disease. The limited number of neuroprotective study carried out so far indicated that metal (iron) chelation therapy could be a viable neuroprotective approach for neurodegenerative disorders. Methanolic and aqueous extract of CA was obtained by conventional soxhlet extraction (temperature: 40-60°C, ratio: 1:30 (w/v), time: 24 h). The IC50 of methanolic and aqueous extract obtained was 0.26 and 0.69 mg mL-1, respectively. Further, Response surface methodology was used to determine the optimum CA extraction condition that gives optimum metal chelating activity. Experiments were designed according to central composite design with four factors (time, temperature, ratio of raw material to solvent and agitation speed) and six central points that derived total 30 runs. In conclusion, run 17 (temperature: 25°C, ratio: 1:45 (w/v), time: 1.5 h and speed: 200 rpm) gave the optimum chelating activity with IC50 0.093 mg mL-1. Asian Network for Scientific Information 2010 Article PeerReviewed application/pdf en http://irep.iium.edu.my/852/1/Centella_asiatica.pdf Amid, Azura and Mohd Salim, Roshan Jahn and Adenan, Mohd Ilham (2010) The factors affecting the extraction condition for neuroprotective activity of centella asiatica evaluated by metal chelating activity assay. Journal of Applied Sciences, 10 (10). pp. 837-842. ISSN 1812-5662 (O), 1812-5654 (P) http://scialert.net/abstract/?doi=jas.2010.837.842 DOI: 10.3923/jas.2010.837.842
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Amid, Azura Mohd Salim, Roshan Jahn Adenan, Mohd Ilham The factors affecting the extraction condition for neuroprotective activity of centella asiatica evaluated by metal chelating activity assay
description	The study was aimed to evaluate the metal chelating activity of CA which will first be assessed for neuroprotective property. Further, response surface methodology will be used to optimize the extraction parameters to yield an optimum metal chelating activity of CA by minimizing the cost of extraction. Centella asiatica (CA) is a rich antioxidant candidate was studied for its potential as a neuroprotective agent to fight against oxidative damage caused by Reactive Oxygen Species (ROS) towards neuronal cells which eventually lead to neurodegenerative diseases such as Alzheimer’s and Parkinson disease. The limited number of neuroprotective study carried out so far indicated that metal (iron) chelation therapy could be a viable neuroprotective approach for neurodegenerative disorders. Methanolic and aqueous extract of CA was obtained by conventional soxhlet extraction (temperature: 40-60°C, ratio: 1:30 (w/v), time: 24 h). The IC50 of methanolic and aqueous extract obtained was 0.26 and 0.69 mg mL-1, respectively. Further, Response surface methodology was used to determine the optimum CA extraction condition that gives optimum metal chelating activity. Experiments were designed according to central composite design with four factors (time, temperature, ratio of raw material to solvent and agitation speed) and six central points that derived total 30 runs. In conclusion, run 17 (temperature: 25°C, ratio: 1:45 (w/v), time: 1.5 h and speed: 200 rpm) gave the optimum chelating activity with IC50 0.093 mg mL-1.
format	Article
author	Amid, Azura Mohd Salim, Roshan Jahn Adenan, Mohd Ilham
author_facet	Amid, Azura Mohd Salim, Roshan Jahn Adenan, Mohd Ilham
author_sort	Amid, Azura
title	The factors affecting the extraction condition for neuroprotective activity of centella asiatica evaluated by metal chelating activity assay
title_short	The factors affecting the extraction condition for neuroprotective activity of centella asiatica evaluated by metal chelating activity assay
title_full	The factors affecting the extraction condition for neuroprotective activity of centella asiatica evaluated by metal chelating activity assay
title_fullStr	The factors affecting the extraction condition for neuroprotective activity of centella asiatica evaluated by metal chelating activity assay
title_full_unstemmed	The factors affecting the extraction condition for neuroprotective activity of centella asiatica evaluated by metal chelating activity assay
title_sort	factors affecting the extraction condition for neuroprotective activity of centella asiatica evaluated by metal chelating activity assay
publisher	Asian Network for Scientific Information
publishDate	2010
url	http://irep.iium.edu.my/852/ http://irep.iium.edu.my/852/ http://irep.iium.edu.my/852/ http://irep.iium.edu.my/852/1/Centella_asiatica.pdf
first_indexed	2023-09-18T20:08:02Z
last_indexed	2023-09-18T20:08:02Z
_version_	1777407290132922368

The factors affecting the extraction condition for neuroprotective activity of centella asiatica evaluated by metal chelating activity assay

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