The effects of temperature on callus induction and regeneration in selected Malaysian rice cultivar Indica

The development of an efficient tissue culture protocol for somatic embryo would facilitate the genetic modification in breeding program. The present study describes the reproducible protocols for three wetland Malaysian rice cultivars (MR232, MR220 and MR220-CL2) and upland rice (Bario) via somatic...

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Main Authors:	Suraiya Mostafiz, Alina Wagiran, Nur Syafiqoh Johan, Nur Saliha Abdullah Zulkifli, Ng, Ja Ming
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:	http://journalarticle.ukm.my/12645/ http://journalarticle.ukm.my/12645/ http://journalarticle.ukm.my/12645/1/07%20Suraiya%20Binte%20Mostafiz.pdf

id	ukm-12645
recordtype	eprints
spelling	ukm-126452019-03-17T11:21:12Z http://journalarticle.ukm.my/12645/ The effects of temperature on callus induction and regeneration in selected Malaysian rice cultivar Indica Suraiya Mostafiz, Alina Wagiran, Nur Syafiqoh Johan, Nur Saliha Abdullah Zulkifli, Ng, Ja Ming The development of an efficient tissue culture protocol for somatic embryo would facilitate the genetic modification in breeding program. The present study describes the reproducible protocols for three wetland Malaysian rice cultivars (MR232, MR220 and MR220-CL2) and upland rice (Bario) via somatic embryogenesis. In the present study, four pre-heat treatments (35, 40, 45 and 50°C) were applied to mature seeds with different imbibition periods (3, 5 and 7 days) prior to culture on MS media with 3 mg/L 2,4-D. The results showed that the cultivars exhibited the highest callus induction percentage from 45°C pre-heated seeds and 3 days imbibition (100%, 96%, 100% and 95% for MR232, MR220, MR220-CL2 and Bario, respectively). Callus was induced early ranging from 3 to 12 days compared to without pre-heat treatment. The regeneration efficiency for MR220 and MR220-CL2 cultivars was significantly higher compared to the control treatment. However, both 45°C and 25°C (control) treatments produced higher plantlet regeneration for MR232 and Bario. This study observed that pre-heat treated seeds prior to callus induction did promote callusing and hence regeneration. These findings can be used to establish a suitable protocol for the in vitro regeneration system for several genetic improvements in the numerous stress tolerances of Malaysian rice. Penerbit Universiti Kebangsaan Malaysia 2018-11 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12645/1/07%20Suraiya%20Binte%20Mostafiz.pdf Suraiya Mostafiz, and Alina Wagiran, and Nur Syafiqoh Johan, and Nur Saliha Abdullah Zulkifli, and Ng, Ja Ming (2018) The effects of temperature on callus induction and regeneration in selected Malaysian rice cultivar Indica. Sains Malaysiana, 47 (11). pp. 2647-2655. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid47bil11_2018/KandunganJilid47Bil11_2018.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	The development of an efficient tissue culture protocol for somatic embryo would facilitate the genetic modification in breeding program. The present study describes the reproducible protocols for three wetland Malaysian rice cultivars (MR232, MR220 and MR220-CL2) and upland rice (Bario) via somatic embryogenesis. In the present study, four pre-heat treatments (35, 40, 45 and 50°C) were applied to mature seeds with different imbibition periods (3, 5 and 7 days) prior to culture on MS media with 3 mg/L 2,4-D. The results showed that the cultivars exhibited the highest callus induction percentage from 45°C pre-heated seeds and 3 days imbibition (100%, 96%, 100% and 95% for MR232, MR220, MR220-CL2 and Bario, respectively). Callus was induced early ranging from 3 to 12 days compared to without pre-heat treatment. The regeneration efficiency for MR220 and MR220-CL2 cultivars was significantly higher compared to the control treatment. However, both 45°C and 25°C (control) treatments produced higher plantlet regeneration for MR232 and Bario. This study observed that pre-heat treated seeds prior to callus induction did promote callusing and hence regeneration. These findings can be used to establish a suitable protocol for the in vitro regeneration system for several genetic improvements in the numerous stress tolerances of Malaysian rice.
format	Article
author	Suraiya Mostafiz, Alina Wagiran, Nur Syafiqoh Johan, Nur Saliha Abdullah Zulkifli, Ng, Ja Ming
spellingShingle	Suraiya Mostafiz, Alina Wagiran, Nur Syafiqoh Johan, Nur Saliha Abdullah Zulkifli, Ng, Ja Ming The effects of temperature on callus induction and regeneration in selected Malaysian rice cultivar Indica
author_facet	Suraiya Mostafiz, Alina Wagiran, Nur Syafiqoh Johan, Nur Saliha Abdullah Zulkifli, Ng, Ja Ming
author_sort	Suraiya Mostafiz,
title	The effects of temperature on callus induction and regeneration in selected Malaysian rice cultivar Indica
title_short	The effects of temperature on callus induction and regeneration in selected Malaysian rice cultivar Indica
title_full	The effects of temperature on callus induction and regeneration in selected Malaysian rice cultivar Indica
title_fullStr	The effects of temperature on callus induction and regeneration in selected Malaysian rice cultivar Indica
title_full_unstemmed	The effects of temperature on callus induction and regeneration in selected Malaysian rice cultivar Indica
title_sort	effects of temperature on callus induction and regeneration in selected malaysian rice cultivar indica
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2018
url	http://journalarticle.ukm.my/12645/ http://journalarticle.ukm.my/12645/ http://journalarticle.ukm.my/12645/1/07%20Suraiya%20Binte%20Mostafiz.pdf
first_indexed	2023-09-18T20:03:04Z
last_indexed	2023-09-18T20:03:04Z
_version_	1777406978078801920

The effects of temperature on callus induction and regeneration in selected Malaysian rice cultivar Indica

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