Fabrication of 3-dimensional Cross-linked Redox Protein/Nanomaterials

In this work, we have described a new 3-dimensional (3D) network of crosslinked Horseradish Peroxidase/Carbon Nanotube (HRP/CNT) on a thiol-modified Au surface in order to build up the effective electrical wiring of the enzyme units with the electrode. This was achieved by the electropolymerization...

Full description

Bibliographic Details
Main Authors:	A. K. M., Kafi, M. M., Yusoff, S. N., Nina
Format:	Conference or Workshop Item
Language:	English
Published:	2015
Subjects:	Q Science (General)
Online Access:	http://umpir.ump.edu.my/id/eprint/11548/ http://umpir.ump.edu.my/id/eprint/11548/7/akm%20kafi-fabrication%20of%203-dimensional.pdf

id	ump-11548
recordtype	eprints
spelling	ump-115482018-07-26T03:30:34Z http://umpir.ump.edu.my/id/eprint/11548/ Fabrication of 3-dimensional Cross-linked Redox Protein/Nanomaterials A. K. M., Kafi M. M., Yusoff S. N., Nina Q Science (General) In this work, we have described a new 3-dimensional (3D) network of crosslinked Horseradish Peroxidase/Carbon Nanotube (HRP/CNT) on a thiol-modified Au surface in order to build up the effective electrical wiring of the enzyme units with the electrode. This was achieved by the electropolymerization of aniline-functionalized carbon nanotubes (CNTs) and 4-aminothiophenol -modified-HRP on a 4-aminothiophenol monolayer-modified Au electrode. The synthesized 3D HRP/CNT networks were characterized with cyclic voltammetry and amperometry, resulting the establishment direct electron transfer between the redox active unit of HRP and the Au surface. Electrochemical measurements reveal that the immobilized HRP exhibits high biological activity and stability and a quasi reversible redox peak of the redox center of HRP was observed at about −0.355 and −0.275 V vs. Ag/AgCl. The electron transfer rate constant, KS and electron transfer co-efficientwere found to be 0.57 s-1 and 0.42, respectively. Based on the electrocatalytic process by direct electrochemistry of HRP, a biosensor for detecting H2O2 was developed. The developed biosensor exhibits excellent electrocatalytic activity for the reduction of H2O2. The proposed biosensor modified with HRP/CNT 3D network displays a broader linear range and a lower detection limit for H2O2 determination. The linear range is from 1.0×10−7 to 1.2×10−4M with a detection limit of 2.2.0×10−8M at 3σ. Moreover, this biosensor exhibits very high sensitivity, good reproducibility and long-time stability. In summary, ease of fabrication, a low cost, fast response and high sensitivity are the main advantages of the new biosensor proposed in this study. These obvious advantages would really help for the real analytical applicability of the proposed biosensor. 2015 Conference or Workshop Item PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/11548/7/akm%20kafi-fabrication%20of%203-dimensional.pdf A. K. M., Kafi and M. M., Yusoff and S. N., Nina (2015) Fabrication of 3-dimensional Cross-linked Redox Protein/Nanomaterials. In: The 3rd International Conference on Advanced Electromaterials, 17-20 November 2015 , Jeju, Korea. p. 1.. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) A. K. M., Kafi M. M., Yusoff S. N., Nina Fabrication of 3-dimensional Cross-linked Redox Protein/Nanomaterials
description	In this work, we have described a new 3-dimensional (3D) network of crosslinked Horseradish Peroxidase/Carbon Nanotube (HRP/CNT) on a thiol-modified Au surface in order to build up the effective electrical wiring of the enzyme units with the electrode. This was achieved by the electropolymerization of aniline-functionalized carbon nanotubes (CNTs) and 4-aminothiophenol -modified-HRP on a 4-aminothiophenol monolayer-modified Au electrode. The synthesized 3D HRP/CNT networks were characterized with cyclic voltammetry and amperometry, resulting the establishment direct electron transfer between the redox active unit of HRP and the Au surface. Electrochemical measurements reveal that the immobilized HRP exhibits high biological activity and stability and a quasi reversible redox peak of the redox center of HRP was observed at about −0.355 and −0.275 V vs. Ag/AgCl. The electron transfer rate constant, KS and electron transfer co-efficientwere found to be 0.57 s-1 and 0.42, respectively. Based on the electrocatalytic process by direct electrochemistry of HRP, a biosensor for detecting H2O2 was developed. The developed biosensor exhibits excellent electrocatalytic activity for the reduction of H2O2. The proposed biosensor modified with HRP/CNT 3D network displays a broader linear range and a lower detection limit for H2O2 determination. The linear range is from 1.0×10−7 to 1.2×10−4M with a detection limit of 2.2.0×10−8M at 3σ. Moreover, this biosensor exhibits very high sensitivity, good reproducibility and long-time stability. In summary, ease of fabrication, a low cost, fast response and high sensitivity are the main advantages of the new biosensor proposed in this study. These obvious advantages would really help for the real analytical applicability of the proposed biosensor.
format	Conference or Workshop Item
author	A. K. M., Kafi M. M., Yusoff S. N., Nina
author_facet	A. K. M., Kafi M. M., Yusoff S. N., Nina
author_sort	A. K. M., Kafi
title	Fabrication of 3-dimensional Cross-linked Redox Protein/Nanomaterials
title_short	Fabrication of 3-dimensional Cross-linked Redox Protein/Nanomaterials
title_full	Fabrication of 3-dimensional Cross-linked Redox Protein/Nanomaterials
title_fullStr	Fabrication of 3-dimensional Cross-linked Redox Protein/Nanomaterials
title_full_unstemmed	Fabrication of 3-dimensional Cross-linked Redox Protein/Nanomaterials
title_sort	fabrication of 3-dimensional cross-linked redox protein/nanomaterials
publishDate	2015
url	http://umpir.ump.edu.my/id/eprint/11548/ http://umpir.ump.edu.my/id/eprint/11548/7/akm%20kafi-fabrication%20of%203-dimensional.pdf
first_indexed	2023-09-18T22:12:25Z
last_indexed	2023-09-18T22:12:25Z
_version_	1777415116134809600

Fabrication of 3-dimensional Cross-linked Redox Protein/Nanomaterials

Similar Items