Multi-response optimization of microwave-assisted extraction of phenolics and flavonoids from chromolaena odorata leaves using response surface methodology

Chromolaena odorata L. is an evergreen bush that may reach 1 m or more as a shrub. Different phenolics and flavonoids that mostly protect cultured skin cells, human small cell lung, reduced oxidative degradation, and breast cancer had been isolated [1, 2]. Microwave-assisted extraction (MAE) was exa...

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Main Authors:	Alara, Oluwaseun Ruth, Nour, A. H., Siti Kholijah, Abdul Mudalip, Olalere, Olusegun Abayomi
Format:	Conference or Workshop Item
Language:	English
Published:	2018
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/21932/ http://umpir.ump.edu.my/id/eprint/21932/1/Conference%20Proceeding%20for%20ICCEIB2018%202.pdf

id	ump-21932
recordtype	eprints
spelling	ump-219322019-09-10T06:49:48Z http://umpir.ump.edu.my/id/eprint/21932/ Multi-response optimization of microwave-assisted extraction of phenolics and flavonoids from chromolaena odorata leaves using response surface methodology Alara, Oluwaseun Ruth Nour, A. H. Siti Kholijah, Abdul Mudalip Olalere, Olusegun Abayomi TP Chemical technology Chromolaena odorata L. is an evergreen bush that may reach 1 m or more as a shrub. Different phenolics and flavonoids that mostly protect cultured skin cells, human small cell lung, reduced oxidative degradation, and breast cancer had been isolated [1, 2]. Microwave-assisted extraction (MAE) was examined to maximize the extraction of total phenolic content (TPC) and total flavonoid content (TFC) from the leaves of Chromolaena odorata using a Box-Behnken design of response surface methodology (RSM). Irradiation time (1-5 min); microwave power level (400- 800 W) and ethanol concentration (20-60%) were the considered MAE variables for the optimal recoveries of TPC and TFC from Chromolaena odorata leaves. Quadratic models satisfactorily described the experimental data and optimized conditions for maximum recoveries of polyphenols and flavonoids. The generated models were significant (p < 0.05) and the optimal MAE variables were irradiation time, 3 min; microwave power level, 493 W; and ethanol concentration of 51% with TPC yield of 88.73 mg GAE/g d.w. and TFC yield of 68.25 mg QE/g d.w. The three investigated variables contributed significantly to the optimal recovery yields. In fact, predicted and experimental data were in good agreement. More so, liquid chromatography-mass spectrometer quadrupole time of flight (LCMS QTOF) and Fourier transform infrared transform (FTIR) analyses were used identify the phenolic compounds and functional groups present in the extract. Thus, the derived models can be used in an industrial extraction process to enhance a large scale extraction system. 2018-08-02 Conference or Workshop Item NonPeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/21932/1/Conference%20Proceeding%20for%20ICCEIB2018%202.pdf Alara, Oluwaseun Ruth and Nour, A. H. and Siti Kholijah, Abdul Mudalip and Olalere, Olusegun Abayomi (2018) Multi-response optimization of microwave-assisted extraction of phenolics and flavonoids from chromolaena odorata leaves using response surface methodology. In: 4th International Conference Of Chemical Engineering And Industrial Biotechnology (ICCEIB 2018), 1-2 Aug 2018 , Seri Pacific Hotel, Kuala Lumpur. pp. 511-512.. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Alara, Oluwaseun Ruth Nour, A. H. Siti Kholijah, Abdul Mudalip Olalere, Olusegun Abayomi Multi-response optimization of microwave-assisted extraction of phenolics and flavonoids from chromolaena odorata leaves using response surface methodology
description	Chromolaena odorata L. is an evergreen bush that may reach 1 m or more as a shrub. Different phenolics and flavonoids that mostly protect cultured skin cells, human small cell lung, reduced oxidative degradation, and breast cancer had been isolated [1, 2]. Microwave-assisted extraction (MAE) was examined to maximize the extraction of total phenolic content (TPC) and total flavonoid content (TFC) from the leaves of Chromolaena odorata using a Box-Behnken design of response surface methodology (RSM). Irradiation time (1-5 min); microwave power level (400- 800 W) and ethanol concentration (20-60%) were the considered MAE variables for the optimal recoveries of TPC and TFC from Chromolaena odorata leaves. Quadratic models satisfactorily described the experimental data and optimized conditions for maximum recoveries of polyphenols and flavonoids. The generated models were significant (p < 0.05) and the optimal MAE variables were irradiation time, 3 min; microwave power level, 493 W; and ethanol concentration of 51% with TPC yield of 88.73 mg GAE/g d.w. and TFC yield of 68.25 mg QE/g d.w. The three investigated variables contributed significantly to the optimal recovery yields. In fact, predicted and experimental data were in good agreement. More so, liquid chromatography-mass spectrometer quadrupole time of flight (LCMS QTOF) and Fourier transform infrared transform (FTIR) analyses were used identify the phenolic compounds and functional groups present in the extract. Thus, the derived models can be used in an industrial extraction process to enhance a large scale extraction system.
format	Conference or Workshop Item
author	Alara, Oluwaseun Ruth Nour, A. H. Siti Kholijah, Abdul Mudalip Olalere, Olusegun Abayomi
author_facet	Alara, Oluwaseun Ruth Nour, A. H. Siti Kholijah, Abdul Mudalip Olalere, Olusegun Abayomi
author_sort	Alara, Oluwaseun Ruth
title	Multi-response optimization of microwave-assisted extraction of phenolics and flavonoids from chromolaena odorata leaves using response surface methodology
title_short	Multi-response optimization of microwave-assisted extraction of phenolics and flavonoids from chromolaena odorata leaves using response surface methodology
title_full	Multi-response optimization of microwave-assisted extraction of phenolics and flavonoids from chromolaena odorata leaves using response surface methodology
title_fullStr	Multi-response optimization of microwave-assisted extraction of phenolics and flavonoids from chromolaena odorata leaves using response surface methodology
title_full_unstemmed	Multi-response optimization of microwave-assisted extraction of phenolics and flavonoids from chromolaena odorata leaves using response surface methodology
title_sort	multi-response optimization of microwave-assisted extraction of phenolics and flavonoids from chromolaena odorata leaves using response surface methodology
publishDate	2018
url	http://umpir.ump.edu.my/id/eprint/21932/ http://umpir.ump.edu.my/id/eprint/21932/1/Conference%20Proceeding%20for%20ICCEIB2018%202.pdf
first_indexed	2023-09-18T22:32:24Z
last_indexed	2023-09-18T22:32:24Z
_version_	1777416373538914304

Multi-response optimization of microwave-assisted extraction of phenolics and flavonoids from chromolaena odorata leaves using response surface methodology

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