Production optimization and characterization of anticancer enzyme L-Asparaginase from Bacillus Sp. using Moringa Oleifera Seeds

L-Asparaginase (L-asparagine amino hydrolase 3.5.1.1) constitutes one of the most biotechnologically and bio medically important group of therapeutic enzymes accounting for about 40% of the total worldwide enzyme sales [1, 2]. During current research L-asparaginase, was produced from Bacillus sp. Cu...

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Main Authors:	Batool, T., Makky, Essam A., M., Khan, M. M., Yusoff
Format:	Conference or Workshop Item
Language:	English
Published:	2018
Subjects:	Q Science (General)
Online Access:	http://umpir.ump.edu.my/id/eprint/23639/ http://umpir.ump.edu.my/id/eprint/23639/1/Production%20optimization%20and%20characterization%20of%20anticancer%20enzyme%20L-Asparaginase%20from%20Bacillus%20Sp.%20using%20Moringa%20Oleifera%20Seeds.pdf

id	ump-23639
recordtype	eprints
spelling	ump-236392019-01-07T08:20:00Z http://umpir.ump.edu.my/id/eprint/23639/ Production optimization and characterization of anticancer enzyme L-Asparaginase from Bacillus Sp. using Moringa Oleifera Seeds Batool, T. Makky, Essam A. M., Khan M. M., Yusoff Q Science (General) L-Asparaginase (L-asparagine amino hydrolase 3.5.1.1) constitutes one of the most biotechnologically and bio medically important group of therapeutic enzymes accounting for about 40% of the total worldwide enzyme sales [1, 2]. During current research L-asparaginase, was produced from Bacillus sp. Cultural parameters affecting the production of L-asparaginase were optimized [3]. Maximal yields of L-asparaginase were recorded from 5-day-old culture grown in nutrient broth in presence of Moringa Oleifera seeds as substrate with initial pH 7.0 at temperature 37◦C [4, 5]. Ammonium chloride (1%) and galactose (1%) served as good nitrogen and carbon sources for L-asparaginase production, respectively. Na+and K+ slightly enhanced the productivity of L-asparaginase [6]. The in vitro antioxidant and antitumor activity of partially purified L-asparaginase enzyme was also studied [7]. The enzyme showed a good scavenging activity against DPPH with IC50 value of 81.645µg/mL [8]. 2018 Conference or Workshop Item PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/23639/1/Production%20optimization%20and%20characterization%20of%20anticancer%20enzyme%20L-Asparaginase%20from%20Bacillus%20Sp.%20using%20Moringa%20Oleifera%20Seeds.pdf Batool, T. and Makky, Essam A. and M., Khan and M. M., Yusoff (2018) Production optimization and characterization of anticancer enzyme L-Asparaginase from Bacillus Sp. using Moringa Oleifera Seeds. In: 4th International Conference Of Chemical Engineering And Industrial Biotechnology (ICCEIB 2018), 1-2 Aug 2018 , Seri Pacific Hotel, Kuala Lumpur. pp. 364-365..
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Batool, T. Makky, Essam A. M., Khan M. M., Yusoff Production optimization and characterization of anticancer enzyme L-Asparaginase from Bacillus Sp. using Moringa Oleifera Seeds
description	L-Asparaginase (L-asparagine amino hydrolase 3.5.1.1) constitutes one of the most biotechnologically and bio medically important group of therapeutic enzymes accounting for about 40% of the total worldwide enzyme sales [1, 2]. During current research L-asparaginase, was produced from Bacillus sp. Cultural parameters affecting the production of L-asparaginase were optimized [3]. Maximal yields of L-asparaginase were recorded from 5-day-old culture grown in nutrient broth in presence of Moringa Oleifera seeds as substrate with initial pH 7.0 at temperature 37◦C [4, 5]. Ammonium chloride (1%) and galactose (1%) served as good nitrogen and carbon sources for L-asparaginase production, respectively. Na+and K+ slightly enhanced the productivity of L-asparaginase [6]. The in vitro antioxidant and antitumor activity of partially purified L-asparaginase enzyme was also studied [7]. The enzyme showed a good scavenging activity against DPPH with IC50 value of 81.645µg/mL [8].
format	Conference or Workshop Item
author	Batool, T. Makky, Essam A. M., Khan M. M., Yusoff
author_facet	Batool, T. Makky, Essam A. M., Khan M. M., Yusoff
author_sort	Batool, T.
title	Production optimization and characterization of anticancer enzyme L-Asparaginase from Bacillus Sp. using Moringa Oleifera Seeds
title_short	Production optimization and characterization of anticancer enzyme L-Asparaginase from Bacillus Sp. using Moringa Oleifera Seeds
title_full	Production optimization and characterization of anticancer enzyme L-Asparaginase from Bacillus Sp. using Moringa Oleifera Seeds
title_fullStr	Production optimization and characterization of anticancer enzyme L-Asparaginase from Bacillus Sp. using Moringa Oleifera Seeds
title_full_unstemmed	Production optimization and characterization of anticancer enzyme L-Asparaginase from Bacillus Sp. using Moringa Oleifera Seeds
title_sort	production optimization and characterization of anticancer enzyme l-asparaginase from bacillus sp. using moringa oleifera seeds
publishDate	2018
url	http://umpir.ump.edu.my/id/eprint/23639/ http://umpir.ump.edu.my/id/eprint/23639/1/Production%20optimization%20and%20characterization%20of%20anticancer%20enzyme%20L-Asparaginase%20from%20Bacillus%20Sp.%20using%20Moringa%20Oleifera%20Seeds.pdf
first_indexed	2023-09-18T22:35:30Z
last_indexed	2023-09-18T22:35:30Z
_version_	1777416567776083968

Production optimization and characterization of anticancer enzyme L-Asparaginase from Bacillus Sp. using Moringa Oleifera Seeds

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