Effect of Temperature and Ph on Glucose Production Using Enzymatic Hydrolysis

Enzymatic hydrolysis is one of the effective methods in glucose production due to enzyme properties which are highly specific and very sensitive. Therefore, this paper was designed to study the effect of temperature and pH on glucose production using enzymatic hydrolysis. By using enzymatic hydrolys...

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Main Author: Ahmad, Siti Solehah
Format: Undergraduates Project Papers
Language:English
Published: 2010
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/3287/
http://umpir.ump.edu.my/id/eprint/3287/1/CD5873_SITI_SOLEHAH_AHMAD.pdf
id ump-3287
recordtype eprints
spelling ump-32872015-03-03T08:00:21Z http://umpir.ump.edu.my/id/eprint/3287/ Effect of Temperature and Ph on Glucose Production Using Enzymatic Hydrolysis Ahmad, Siti Solehah TP Chemical technology Enzymatic hydrolysis is one of the effective methods in glucose production due to enzyme properties which are highly specific and very sensitive. Therefore, this paper was designed to study the effect of temperature and pH on glucose production using enzymatic hydrolysis. By using enzymatic hydrolysis, cellulase from Trichoderma reesei was used and supplemented with cellobiase from Aspergillus niger for increasing the glucose production. Cellulose as a substrate was hydrolyzed by these enzymes to produce glucose and the amount of glucose production was determined by high-performance liquid chromatography (HPLC). The result showed that the highest glucose concentration was produced at the temperature of 50°C and pH 5.0 while the lowest glucose production was produced at 30°C and pH 6.5 which were 21.09 mg/ml and 3.55 mg/ml, respectively. By changes the temperature and pH above or below 50°C and pH 5.0 results in low glucose production because the enzyme was lose their three-dimensional functional shape due to disruption from unstable condition of the environment system. As a conclusion, all the requirement for complementary in the configuration of cellulose and enzyme must be considered seriously for the highest glucose production. 2010-04 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/3287/1/CD5873_SITI_SOLEHAH_AHMAD.pdf Ahmad, Siti Solehah (2010) Effect of Temperature and Ph on Glucose Production Using Enzymatic Hydrolysis. Faculty of Chemical & Natural Resources Engineering , Universiti Malaysia Pahang .
repository_type Digital Repository
institution_category Local University
institution Universiti Malaysia Pahang
building UMP Institutional Repository
collection Online Access
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Ahmad, Siti Solehah
Effect of Temperature and Ph on Glucose Production Using Enzymatic Hydrolysis
description Enzymatic hydrolysis is one of the effective methods in glucose production due to enzyme properties which are highly specific and very sensitive. Therefore, this paper was designed to study the effect of temperature and pH on glucose production using enzymatic hydrolysis. By using enzymatic hydrolysis, cellulase from Trichoderma reesei was used and supplemented with cellobiase from Aspergillus niger for increasing the glucose production. Cellulose as a substrate was hydrolyzed by these enzymes to produce glucose and the amount of glucose production was determined by high-performance liquid chromatography (HPLC). The result showed that the highest glucose concentration was produced at the temperature of 50°C and pH 5.0 while the lowest glucose production was produced at 30°C and pH 6.5 which were 21.09 mg/ml and 3.55 mg/ml, respectively. By changes the temperature and pH above or below 50°C and pH 5.0 results in low glucose production because the enzyme was lose their three-dimensional functional shape due to disruption from unstable condition of the environment system. As a conclusion, all the requirement for complementary in the configuration of cellulose and enzyme must be considered seriously for the highest glucose production.
format Undergraduates Project Papers
author Ahmad, Siti Solehah
author_facet Ahmad, Siti Solehah
author_sort Ahmad, Siti Solehah
title Effect of Temperature and Ph on Glucose Production Using Enzymatic Hydrolysis
title_short Effect of Temperature and Ph on Glucose Production Using Enzymatic Hydrolysis
title_full Effect of Temperature and Ph on Glucose Production Using Enzymatic Hydrolysis
title_fullStr Effect of Temperature and Ph on Glucose Production Using Enzymatic Hydrolysis
title_full_unstemmed Effect of Temperature and Ph on Glucose Production Using Enzymatic Hydrolysis
title_sort effect of temperature and ph on glucose production using enzymatic hydrolysis
publishDate 2010
url http://umpir.ump.edu.my/id/eprint/3287/
http://umpir.ump.edu.my/id/eprint/3287/1/CD5873_SITI_SOLEHAH_AHMAD.pdf
first_indexed 2023-09-18T21:57:28Z
last_indexed 2023-09-18T21:57:28Z
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