The extraction of amino acids from papaya leaves using supercritical carbon dioxide with water

In this study, a new extraction method for the isolation papain form papaya leaves (carica papaya sp.) is being developed using supercritical carbon dioxide (SC-CO2) with water. The parameters of temperature, extraction time and comparison yield with hot water extraction were studied. Results indica...

Full description

Bibliographic Details
Main Author:	Nur Hazila, Mohamad Azmi
Format:	Undergraduates Project Papers
Language:	English
Published:	2010
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/3392/ http://umpir.ump.edu.my/id/eprint/3392/ http://umpir.ump.edu.my/id/eprint/3392/1/CD5896_NUR_HAZILA.pdf

id	ump-3392
recordtype	eprints
spelling	ump-33922015-03-03T08:00:54Z http://umpir.ump.edu.my/id/eprint/3392/ The extraction of amino acids from papaya leaves using supercritical carbon dioxide with water Nur Hazila, Mohamad Azmi TP Chemical technology In this study, a new extraction method for the isolation papain form papaya leaves (carica papaya sp.) is being developed using supercritical carbon dioxide (SC-CO2) with water. The parameters of temperature, extraction time and comparison yield with hot water extraction were studied. Results indicated that temperature and extraction time had significant effect on the extraction yield. The highest extraction yield (0.56mol/liter) amino acid was obtained at 300 bar, 50 °C and 2hours. Extracted yields were then analysed by UV-vis spectrophotometer at 660nm and casein as a substrate. The composition of the extracted yields was greatly impacted by the operating conditions. At the best extraction condition (300 bar, 50 °C and 2 hours), amino acid had the highest concentration (0.56mol/liter) among all the other detected samples. The present findings showed that papaya leaves is a potential source of papain enzymes compounds. In addition, supercritical carbon dioxide extraction is a promising a high yield and alternative process for construct the papain compounds from papaya (carica papaya sp.) leaves which is by-product raw material. For further study, High Performance Liquid Chromatography (HPLC) should be using to analyses the enzyme to more accurate result. 2010-04 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/3392/1/CD5896_NUR_HAZILA.pdf Nur Hazila, Mohamad Azmi (2010) The extraction of amino acids from papaya leaves using supercritical carbon dioxide with water. Faculty of Chemical and Natural Resources Engineering , Universiti Malaysia Pahang . http://iportal.ump.edu.my/lib/item?id=chamo:58548&theme=UMP2
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Nur Hazila, Mohamad Azmi The extraction of amino acids from papaya leaves using supercritical carbon dioxide with water
description	In this study, a new extraction method for the isolation papain form papaya leaves (carica papaya sp.) is being developed using supercritical carbon dioxide (SC-CO2) with water. The parameters of temperature, extraction time and comparison yield with hot water extraction were studied. Results indicated that temperature and extraction time had significant effect on the extraction yield. The highest extraction yield (0.56mol/liter) amino acid was obtained at 300 bar, 50 °C and 2hours. Extracted yields were then analysed by UV-vis spectrophotometer at 660nm and casein as a substrate. The composition of the extracted yields was greatly impacted by the operating conditions. At the best extraction condition (300 bar, 50 °C and 2 hours), amino acid had the highest concentration (0.56mol/liter) among all the other detected samples. The present findings showed that papaya leaves is a potential source of papain enzymes compounds. In addition, supercritical carbon dioxide extraction is a promising a high yield and alternative process for construct the papain compounds from papaya (carica papaya sp.) leaves which is by-product raw material. For further study, High Performance Liquid Chromatography (HPLC) should be using to analyses the enzyme to more accurate result.
format	Undergraduates Project Papers
author	Nur Hazila, Mohamad Azmi
author_facet	Nur Hazila, Mohamad Azmi
author_sort	Nur Hazila, Mohamad Azmi
title	The extraction of amino acids from papaya leaves using supercritical carbon dioxide with water
title_short	The extraction of amino acids from papaya leaves using supercritical carbon dioxide with water
title_full	The extraction of amino acids from papaya leaves using supercritical carbon dioxide with water
title_fullStr	The extraction of amino acids from papaya leaves using supercritical carbon dioxide with water
title_full_unstemmed	The extraction of amino acids from papaya leaves using supercritical carbon dioxide with water
title_sort	extraction of amino acids from papaya leaves using supercritical carbon dioxide with water
publishDate	2010
url	http://umpir.ump.edu.my/id/eprint/3392/ http://umpir.ump.edu.my/id/eprint/3392/ http://umpir.ump.edu.my/id/eprint/3392/1/CD5896_NUR_HAZILA.pdf
first_indexed	2023-09-18T21:57:39Z
last_indexed	2023-09-18T21:57:39Z
_version_	1777414186783997952

The extraction of amino acids from papaya leaves using supercritical carbon dioxide with water

Similar Items