Production of poly-hydroxy butyrate (PHB) by Bacilius Cereus using banana stem as a substrate

The research was focused on delignification process and production of (Poly-β-hydroxybutyrate) PHB by Bacillus cereus using banana stem wastes as substrate. This study is divided into three parts which are determination growth curve of Bacillus cereus, lignin analysis using Klason method and determi...

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Main Author:	Nurul Hanim, Mat Noor
Format:	Undergraduates Project Papers
Language:	English
Published:	2010
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/3421/ http://umpir.ump.edu.my/id/eprint/3421/1/CD5946_NURUL_HANIM_BINTI_MAT_NOOR_X.pdf

id	ump-3421
recordtype	eprints
spelling	ump-34212015-03-03T08:01:04Z http://umpir.ump.edu.my/id/eprint/3421/ Production of poly-hydroxy butyrate (PHB) by Bacilius Cereus using banana stem as a substrate Nurul Hanim, Mat Noor TP Chemical technology The research was focused on delignification process and production of (Poly-β-hydroxybutyrate) PHB by Bacillus cereus using banana stem wastes as substrate. This study is divided into three parts which are determination growth curve of Bacillus cereus, lignin analysis using Klason method and determination of PHB. Delignification process is functioning to degrade lignin from banana stem to get cellulose that can be used as carbon sources in production of PHB. PHB is a carbon storage polymer that is widely distributed among prokaryotes including Bacilius cereus. The delignification methods are divided into three parts which are preparation of raw material and microorganisms, fermentation process and lignin analysis. Fermentation was carried out aerobically at optimum temperature 37 ˚C and 250 rpm of agitation speed for 2 days. In this process, Klason method was used to identify the percentage of lignin degraded. Based on the analysis, it shows that Bacillus cereus is able to degrade lignin from banana stem wastes. The average lignins degrade by Bacillus cereus was 40.175%. Meanwhile, for production of PHB methods were divided into four parts which are fermentation, pre-treatment, extraction and analysis. In extraction process, ultrasonic treatment, H2SO4, HCl and chloroform was used to break the granule and cell wall. In this study, the best PHB production and percentage yield of this strain was determined. The PHB production was 27.897 mg/ml and the percentage yield was 55.48% at 48 hours. After 48 hours there was a decrease in PHB yield because of the bacteria used PHB as a source of carbon and nitrogen, causing an unsuitable condition due to inadequate nitrogen and carbon sources in the medium. 2010-04 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/3421/1/CD5946_NURUL_HANIM_BINTI_MAT_NOOR_X.pdf Nurul Hanim, Mat Noor (2010) Production of poly-hydroxy butyrate (PHB) by Bacilius Cereus using banana stem as a substrate. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang.
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Nurul Hanim, Mat Noor Production of poly-hydroxy butyrate (PHB) by Bacilius Cereus using banana stem as a substrate
description	The research was focused on delignification process and production of (Poly-β-hydroxybutyrate) PHB by Bacillus cereus using banana stem wastes as substrate. This study is divided into three parts which are determination growth curve of Bacillus cereus, lignin analysis using Klason method and determination of PHB. Delignification process is functioning to degrade lignin from banana stem to get cellulose that can be used as carbon sources in production of PHB. PHB is a carbon storage polymer that is widely distributed among prokaryotes including Bacilius cereus. The delignification methods are divided into three parts which are preparation of raw material and microorganisms, fermentation process and lignin analysis. Fermentation was carried out aerobically at optimum temperature 37 ˚C and 250 rpm of agitation speed for 2 days. In this process, Klason method was used to identify the percentage of lignin degraded. Based on the analysis, it shows that Bacillus cereus is able to degrade lignin from banana stem wastes. The average lignins degrade by Bacillus cereus was 40.175%. Meanwhile, for production of PHB methods were divided into four parts which are fermentation, pre-treatment, extraction and analysis. In extraction process, ultrasonic treatment, H2SO4, HCl and chloroform was used to break the granule and cell wall. In this study, the best PHB production and percentage yield of this strain was determined. The PHB production was 27.897 mg/ml and the percentage yield was 55.48% at 48 hours. After 48 hours there was a decrease in PHB yield because of the bacteria used PHB as a source of carbon and nitrogen, causing an unsuitable condition due to inadequate nitrogen and carbon sources in the medium.
format	Undergraduates Project Papers
author	Nurul Hanim, Mat Noor
author_facet	Nurul Hanim, Mat Noor
author_sort	Nurul Hanim, Mat Noor
title	Production of poly-hydroxy butyrate (PHB) by Bacilius Cereus using banana stem as a substrate
title_short	Production of poly-hydroxy butyrate (PHB) by Bacilius Cereus using banana stem as a substrate
title_full	Production of poly-hydroxy butyrate (PHB) by Bacilius Cereus using banana stem as a substrate
title_fullStr	Production of poly-hydroxy butyrate (PHB) by Bacilius Cereus using banana stem as a substrate
title_full_unstemmed	Production of poly-hydroxy butyrate (PHB) by Bacilius Cereus using banana stem as a substrate
title_sort	production of poly-hydroxy butyrate (phb) by bacilius cereus using banana stem as a substrate
publishDate	2010
url	http://umpir.ump.edu.my/id/eprint/3421/ http://umpir.ump.edu.my/id/eprint/3421/1/CD5946_NURUL_HANIM_BINTI_MAT_NOOR_X.pdf
first_indexed	2023-09-18T21:57:43Z
last_indexed	2023-09-18T21:57:43Z
_version_	1777414190421508096

Production of poly-hydroxy butyrate (PHB) by Bacilius Cereus using banana stem as a substrate

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