Effect of imidazole concentration and type of metal ion on the purification of recombinant green fluorescent protein using an affinity chromatography
The purification of protein using IMAC can be influenced by imidazole concentration and type of metal ion. Different type of metal ion and different imidazole concentration will give different result of purity and yield for target protein. The strength of binding between histidine- tagged protein an...
| Main Author: | |
|---|---|
| Format: | Undergraduates Project Papers |
| Language: | English |
| Published: |
2014
|
| Subjects: | |
| Online Access: | http://umpir.ump.edu.my/id/eprint/8853/ http://umpir.ump.edu.my/id/eprint/8853/ http://umpir.ump.edu.my/id/eprint/8853/1/CD8547.pdf |