Characterisation and stability of pigments extracted from sargassum binderi obtained from Semporna, Sabah

This study was carried out to identify the pigment extracted from Malaysian brown seaweed, Sargassum binderi and its stability in various conditions. Pigments were extracted using methanol:chloroform:water (4:2:1, v/v/v), which is part of fucoidan extraction process, where the pigments were waste. C...

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Main Authors:	Wu, Hon Yip, Lim, Seng Joe, Wan Aida Wan Mustapha, Mohamad Yusof Maskat, Mamot Said
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2014
Online Access:	http://journalarticle.ukm.my/7669/ http://journalarticle.ukm.my/7669/ http://journalarticle.ukm.my/7669/1/08_Wu_Hon_Yip.pdf

id	ukm-7669
recordtype	eprints
spelling	ukm-76692016-12-14T06:44:49Z http://journalarticle.ukm.my/7669/ Characterisation and stability of pigments extracted from sargassum binderi obtained from Semporna, Sabah Wu, Hon Yip Lim, Seng Joe Wan Aida Wan Mustapha, Mohamad Yusof Maskat, Mamot Said, This study was carried out to identify the pigment extracted from Malaysian brown seaweed, Sargassum binderi and its stability in various conditions. Pigments were extracted using methanol:chloroform:water (4:2:1, v/v/v), which is part of fucoidan extraction process, where the pigments were waste. Carotenoid and chlorophyll were found in the extract using UV-vis spectrophotometer (420 and 672 nm, respectively). Fucoxanthin was identified as the carotenoid present using HPLC, while its functional groups and structure were determined using FTIR and 1H NMR, respectively. The fucoxanthin-rich extract stability was tested on different pH (pH1-13), light exposure (dark and light) and storage temperature (4ºC, 25ºC and 50ºC). The stability tests showed that it was most stable at pH5-7, stored in dark condition and at the storage temperature of 4ºC and 25ºC. The fucoxanthin-rich extract from Sargassum binderi has potential to be applied as bioingredient and functional food as it is stable in normal storage conditions. Universiti Kebangsaan Malaysia 2014-09 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/7669/1/08_Wu_Hon_Yip.pdf Wu, Hon Yip and Lim, Seng Joe and Wan Aida Wan Mustapha, and Mohamad Yusof Maskat, and Mamot Said, (2014) Characterisation and stability of pigments extracted from sargassum binderi obtained from Semporna, Sabah. Sains Malaysiana, 43 (9). pp. 1345-1354. ISSN 0126-6039 http://www.ukm.my/jsm/
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	This study was carried out to identify the pigment extracted from Malaysian brown seaweed, Sargassum binderi and its stability in various conditions. Pigments were extracted using methanol:chloroform:water (4:2:1, v/v/v), which is part of fucoidan extraction process, where the pigments were waste. Carotenoid and chlorophyll were found in the extract using UV-vis spectrophotometer (420 and 672 nm, respectively). Fucoxanthin was identified as the carotenoid present using HPLC, while its functional groups and structure were determined using FTIR and 1H NMR, respectively. The fucoxanthin-rich extract stability was tested on different pH (pH1-13), light exposure (dark and light) and storage temperature (4ºC, 25ºC and 50ºC). The stability tests showed that it was most stable at pH5-7, stored in dark condition and at the storage temperature of 4ºC and 25ºC. The fucoxanthin-rich extract from Sargassum binderi has potential to be applied as bioingredient and functional food as it is stable in normal storage conditions.
format	Article
author	Wu, Hon Yip Lim, Seng Joe Wan Aida Wan Mustapha, Mohamad Yusof Maskat, Mamot Said,
spellingShingle	Wu, Hon Yip Lim, Seng Joe Wan Aida Wan Mustapha, Mohamad Yusof Maskat, Mamot Said, Characterisation and stability of pigments extracted from sargassum binderi obtained from Semporna, Sabah
author_facet	Wu, Hon Yip Lim, Seng Joe Wan Aida Wan Mustapha, Mohamad Yusof Maskat, Mamot Said,
author_sort	Wu, Hon Yip
title	Characterisation and stability of pigments extracted from sargassum binderi obtained from Semporna, Sabah
title_short	Characterisation and stability of pigments extracted from sargassum binderi obtained from Semporna, Sabah
title_full	Characterisation and stability of pigments extracted from sargassum binderi obtained from Semporna, Sabah
title_fullStr	Characterisation and stability of pigments extracted from sargassum binderi obtained from Semporna, Sabah
title_full_unstemmed	Characterisation and stability of pigments extracted from sargassum binderi obtained from Semporna, Sabah
title_sort	characterisation and stability of pigments extracted from sargassum binderi obtained from semporna, sabah
publisher	Universiti Kebangsaan Malaysia
publishDate	2014
url	http://journalarticle.ukm.my/7669/ http://journalarticle.ukm.my/7669/ http://journalarticle.ukm.my/7669/1/08_Wu_Hon_Yip.pdf
first_indexed	2023-09-18T19:50:18Z
last_indexed	2023-09-18T19:50:18Z
_version_	1777406174165991424

Characterisation and stability of pigments extracted from sargassum binderi obtained from Semporna, Sabah

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