α-Glucosidase and α-amylase Inhibitory Constituents of Tinospora crispa: Isolation and Chemical Profile Confirmation by Ultra-high Performance Liquid Chromatography-quadrupole Time-of-flight/mass spectrometry

Tinospora crispa has been used in Malaysia to treat diabetes mellitus. We evaluated the effects of compounds isolated from T. crispa vines on the starch digestive enzymes, α-glucosidase and α-amylase. Borapetoside C was the most potent α-glucosidase inhibitor (IC50 = 0.0527 ± 0.008 mg/ml), followed...

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Main Authors:	Hazrulrizawati, Hamid, M. M., Yusoff, M., Liu, M. R., Karim
Format:	Article
Language:	English English
Published:	Elsevier Ltd 2015
Subjects:	Q Science (General)
Online Access:	http://umpir.ump.edu.my/id/eprint/9262/ http://umpir.ump.edu.my/id/eprint/9262/ http://umpir.ump.edu.my/id/eprint/9262/ http://umpir.ump.edu.my/id/eprint/9262/1/fist-2015-mashitah- http://umpir.ump.edu.my/id/eprint/9262/2/a-Glucosidase%20and%20a-amylase%20Inhibitory%20constituents%20of%20Tinospora%20crispa-%20Isolation%20and%20Chemical%20Profile%20Confirmation%20by%20Ultra-High%20Performance%20Liquid%20Chromatography-Quadrupole%20Time-Of-Flight-Mass%20Spectrometry.pdf

id	ump-9262
recordtype	eprints
spelling	ump-92622018-07-26T07:53:06Z http://umpir.ump.edu.my/id/eprint/9262/ α-Glucosidase and α-amylase Inhibitory Constituents of Tinospora crispa: Isolation and Chemical Profile Confirmation by Ultra-high Performance Liquid Chromatography-quadrupole Time-of-flight/mass spectrometry Hazrulrizawati, Hamid M. M., Yusoff M., Liu M. R., Karim Q Science (General) Tinospora crispa has been used in Malaysia to treat diabetes mellitus. We evaluated the effects of compounds isolated from T. crispa vines on the starch digestive enzymes, α-glucosidase and α-amylase. Borapetoside C was the most potent α-glucosidase inhibitor (IC50 = 0.0527 ± 0.008 mg/ml), followed by 4-hydroxy benzaldehyde (IC50 = 0.557 ± 0.004 mg/ml), and the alkaloids lysicamine and liriodenine (IC50 = 0.562 ± 0.003 mg/ml). Borapetoside C also potently inhibited α-amylase inhibitory activity (IC50 = 0.775 ± 0.005 mg/ml). The relationship between compound activity and content in the extracts was determined by ultra-high performance liquid chromatography-quadrupole time-of-flight/mass spectrometry (UPLC-QToF/MS). These results suggest that borapetoside C is the main primary active component of T. crispa extracts, mediating α-glucosidase and α-amylase inhibition. Elsevier Ltd 2015 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/9262/1/fist-2015-mashitah- application/pdf en http://umpir.ump.edu.my/id/eprint/9262/2/a-Glucosidase%20and%20a-amylase%20Inhibitory%20constituents%20of%20Tinospora%20crispa-%20Isolation%20and%20Chemical%20Profile%20Confirmation%20by%20Ultra-High%20Performance%20Liquid%20Chromatography-Quadrupole%20Time-Of-Flight-Mass%20Spectrometry.pdf Hazrulrizawati, Hamid and M. M., Yusoff and M., Liu and M. R., Karim (2015) α-Glucosidase and α-amylase Inhibitory Constituents of Tinospora crispa: Isolation and Chemical Profile Confirmation by Ultra-high Performance Liquid Chromatography-quadrupole Time-of-flight/mass spectrometry. Journal of Functional Foods, 16. pp. 74-80. ISSN 1756-4646 http://dx.doi.org/10.1016/j.jff.2015.04.011 doi: 10.1016/j.jff.2015.04.011
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English English
topic	Q Science (General)
spellingShingle	Q Science (General) Hazrulrizawati, Hamid M. M., Yusoff M., Liu M. R., Karim α-Glucosidase and α-amylase Inhibitory Constituents of Tinospora crispa: Isolation and Chemical Profile Confirmation by Ultra-high Performance Liquid Chromatography-quadrupole Time-of-flight/mass spectrometry
description	Tinospora crispa has been used in Malaysia to treat diabetes mellitus. We evaluated the effects of compounds isolated from T. crispa vines on the starch digestive enzymes, α-glucosidase and α-amylase. Borapetoside C was the most potent α-glucosidase inhibitor (IC50 = 0.0527 ± 0.008 mg/ml), followed by 4-hydroxy benzaldehyde (IC50 = 0.557 ± 0.004 mg/ml), and the alkaloids lysicamine and liriodenine (IC50 = 0.562 ± 0.003 mg/ml). Borapetoside C also potently inhibited α-amylase inhibitory activity (IC50 = 0.775 ± 0.005 mg/ml). The relationship between compound activity and content in the extracts was determined by ultra-high performance liquid chromatography-quadrupole time-of-flight/mass spectrometry (UPLC-QToF/MS). These results suggest that borapetoside C is the main primary active component of T. crispa extracts, mediating α-glucosidase and α-amylase inhibition.
format	Article
author	Hazrulrizawati, Hamid M. M., Yusoff M., Liu M. R., Karim
author_facet	Hazrulrizawati, Hamid M. M., Yusoff M., Liu M. R., Karim
author_sort	Hazrulrizawati, Hamid
title	α-Glucosidase and α-amylase Inhibitory Constituents of Tinospora crispa: Isolation and Chemical Profile Confirmation by Ultra-high Performance Liquid Chromatography-quadrupole Time-of-flight/mass spectrometry
title_short	α-Glucosidase and α-amylase Inhibitory Constituents of Tinospora crispa: Isolation and Chemical Profile Confirmation by Ultra-high Performance Liquid Chromatography-quadrupole Time-of-flight/mass spectrometry
title_full	α-Glucosidase and α-amylase Inhibitory Constituents of Tinospora crispa: Isolation and Chemical Profile Confirmation by Ultra-high Performance Liquid Chromatography-quadrupole Time-of-flight/mass spectrometry
title_fullStr	α-Glucosidase and α-amylase Inhibitory Constituents of Tinospora crispa: Isolation and Chemical Profile Confirmation by Ultra-high Performance Liquid Chromatography-quadrupole Time-of-flight/mass spectrometry
title_full_unstemmed	α-Glucosidase and α-amylase Inhibitory Constituents of Tinospora crispa: Isolation and Chemical Profile Confirmation by Ultra-high Performance Liquid Chromatography-quadrupole Time-of-flight/mass spectrometry
title_sort	α-glucosidase and α-amylase inhibitory constituents of tinospora crispa: isolation and chemical profile confirmation by ultra-high performance liquid chromatography-quadrupole time-of-flight/mass spectrometry
publisher	Elsevier Ltd
publishDate	2015
url	http://umpir.ump.edu.my/id/eprint/9262/ http://umpir.ump.edu.my/id/eprint/9262/ http://umpir.ump.edu.my/id/eprint/9262/ http://umpir.ump.edu.my/id/eprint/9262/1/fist-2015-mashitah- http://umpir.ump.edu.my/id/eprint/9262/2/a-Glucosidase%20and%20a-amylase%20Inhibitory%20constituents%20of%20Tinospora%20crispa-%20Isolation%20and%20Chemical%20Profile%20Confirmation%20by%20Ultra-High%20Performance%20Liquid%20Chromatography-Quadrupole%20Time-Of-Flight-Mass%20Spectrometry.pdf
first_indexed	2023-09-18T22:07:39Z
last_indexed	2023-09-18T22:07:39Z
_version_	1777414815847809024

α-Glucosidase and α-amylase Inhibitory Constituents of Tinospora crispa: Isolation and Chemical Profile Confirmation by Ultra-high Performance Liquid Chromatography-quadrupole Time-of-flight/mass spectrometry

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